On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of <i>Polygonum viviparum</i>

Qian, Zhengming; Cheng, Xin-jie; Wang, Qiao; Hu, Qi; Jin, Li-ling; Ma, Yun; Jiasheng, Xie; Li, Deqiang

doi:10.1039/d2ra08247k

Cited by 3 publications

(3 citation statements)

References 18 publications

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“…Referencing previous methodologies [40], we weighed approximately 0.5 g of the sample powder three times, and placed each portion into a separate 15 mL centrifuge tube. To each tube, we added 5 mL of ultrapure water, 50% methanol, and anhydrous methanol, respectively.…”

Section: Offline Extraction Of Samplementioning

confidence: 99%

Screening Antioxidant Components in Different Parts of Dandelion Using Online Gradient Pressure Liquid Extraction Coupled with High-Performance Liquid Chromatography Antioxidant Analysis System and Molecular Simulations

Cao,

Li,

Xie

et al. 2024

Molecules

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Utilizing online gradient pressure liquid extraction (OGPLE) coupled with a high-performance liquid chromatography antioxidant analysis system, we examined the antioxidative active components present in both the aerial parts and roots of dandelion. By optimizing the chromatographic conditions, we identified the ferric reducing–antioxidant power system as the most suitable for online antioxidant reactions in dandelion. Compared to offline ultrasonic extraction, the OGPLE method demonstrated superior efficiency in extracting chemical components with varying polarities from the samples. Liquid chromatography–mass spectrometry revealed twelve compounds within the dandelion samples, with nine demonstrating considerable antioxidant efficacy. Of these, the aerial parts and roots of dandelion contained nine and four antioxidant constituents, respectively. Additionally, molecular docking studies were carried out to investigate the interaction between these nine antioxidants and four proteins associated with oxidative stress (glutathione peroxidase, inducible nitric oxide synthase, superoxide dismutase, and xanthine oxidase). The nine antioxidant compounds displayed notable binding affinities below −5.0 kcal/mol with the selected proteins, suggesting potential receptor–ligand interactions. These findings contribute to enhancing our understanding of dandelion and provide a comprehensive methodology for screening the natural antioxidant components from herbs.

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Section: Offline Extraction Of Samplementioning

confidence: 99%

Screening Antioxidant Components in Different Parts of Dandelion Using Online Gradient Pressure Liquid Extraction Coupled with High-Performance Liquid Chromatography Antioxidant Analysis System and Molecular Simulations

Cao,

Li,

Xie

et al. 2024

Molecules

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“…Its rhizome is considered to be primarily responsible for its pharmacological properties (e.g., antipyretic, antidiarrheal, menstruation-regulating, and hemostasis effects), and it is also an important food source for the host larvae of Cordyceps sinensis [ 5 ]. It has high medicinal, feeding, and ecological value [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

“…Some previous studies have identified volatile oils, organic acids, and sterols from B. vivipara via traditional methods. However, there are abundant primary and secondary metabolites in plants, which comprise only some of them [ 6 ]. Metabolomics technology is mainly used to identify functional secondary metabolites and metabolic pathways [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Revealing Medicinal Constituents of Bistorta vivipara Based on Non-Targeted Metabolomics and 16S rDNA Gene Sequencing Technology

He,

Tang,

Cao

et al. 2024

Molecules

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Bistorta vivipara is a medicinal plant with a long history, but there are few studies on the effects of its medicinal components and endophytic bacteria on the accumulation of secondary metabolites. Therefore, in this study, non-targeted metabolomics techniques and 16s rDNA techniques were used to study B. vivipara from different regions. A total of 1290 metabolites and 437 differential metabolites were identified from all samples. Among them, flavonoids, isoflavonoids, and benzopyrans are the main medicinal components of B. vivipara; these have potential anticancer, antiviral, and antioxidant properties, as well as potential applications for the treatment of atrial fibrillation. In addition, irigenin, an important medicinal component, was identified for the first time. The endophytic bacterial communities in the root tissues of B. vivipara from different regions were also different in composition and richness. Hierarchical clustering heat map analysis showed that Proteobacteria and Actinobacteriota bacteria significantly affected the accumulation of many medicinal components in the roots of B. vivipara.

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Analysis of Antioxidants from Three Parts of Polygonum Chinense based on Online Extraction High Performance Liquid Chromatography Antioxidant Analysis System

Qian,

Huang,

et al. 2024

Chemistry & Biodiversity

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In the current study, a rapid online extraction combined with high‐performance liquid chromatography (HPLC) antioxidant analysis approach was developed to explore the antioxidant ingredients in three different parts of Polygonum Chinense. A total of 22 chromatographic peaks were found, among which 8 components were found for the first time. And among these 22 peaks, 19 of them were demonstrated as antioxidants. Furthermo, all three parts from Polygonum Chinense exhibited antioxidant activity (13.19, 3.89, and 19.85 mg CE/g extract, for the leaf, stem, and flower, respectively) and the antioxidant activity values of the leaf and flower were more than 3 times that of the stem. The leaf, flower, and stem of Polygonum Chinense had 17, 13, and 15 antioxidant components, respectively. Among them, neochlorogenic acid (2) and quercetin‐3‐O‐malonylglucoside (18) were considered as the main antioxidants in the leaf of Polygonum Chinense. In terms of flower, geraniin (10) and quercetin‐3‐O‐malonylglucoside (18) were proved as the major antioxidants. This is the first report on the antioxidant components from different parts of Polygonum Chinense. It provides foundational scientific data for the continued exploration of chemical and pharmacological study of Polygonum Chinense, beneficial for the future product development and quality evaluation improvement of Polygonum Chinense.

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On-line pre-column FRAP-based antioxidant reaction coupled with HPLC-DAD-TOF/MS for rapid screening of natural antioxidants from different parts of Polygonum viviparum

Abstract: Polygonum viviparum L. (PV) is a widely used resource plant with high medicinal, feeding and ecological values.

Cited by 3 publications

References 18 publications

Screening Antioxidant Components in Different Parts of Dandelion Using Online Gradient Pressure Liquid Extraction Coupled with High-Performance Liquid Chromatography Antioxidant Analysis System and Molecular Simulations

Screening Antioxidant Components in Different Parts of Dandelion Using Online Gradient Pressure Liquid Extraction Coupled with High-Performance Liquid Chromatography Antioxidant Analysis System and Molecular Simulations

Revealing Medicinal Constituents of Bistorta vivipara Based on Non-Targeted Metabolomics and 16S rDNA Gene Sequencing Technology

Analysis of Antioxidants from Three Parts of Polygonum Chinense based on Online Extraction High Performance Liquid Chromatography Antioxidant Analysis System

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