On-line sequential detection by inductively coupled plasma atomic emission spectrometry of trace elements after liquid chromatography of biological fluids

Gardiner, Philip H. E.; Braetter, P.; Gercken, Bertold; Tomiak, A.

doi:10.1039/ja9870200375

Cited by 18 publications

(12 citation statements)

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“…Moving average smoothing was applied next (n~5) and finally the mass flow was computed from each point in the chromatogram using the measured ratio as R m in eqn. (9). Fig.…”

Section: On-line Isotope Dilution Analysis For Fe Cu and Zn In Human ...mentioning

confidence: 99%

Speciation of essential elements in human serum using anion-exchange chromatography coupled to post-column isotope dilution analysis with double focusing ICP-MS

Muñiz

Marchante-Gayón

Alonso

et al. 2001

J. Anal. At. Spectrom.

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The potential of anion-exchange chromatography coupled to double focusing ICP-MS via a post-column isotope dilution analysis mode for the quantitative speciation of Fe, Cu and Zn in human serum is described. Protein separation was achieved with an anion-exchange column (Mono-Q HR 5/5) while the mobile phase consisted of a gradient of initial buffer solution (0.05 M Tris-HCl, pH~7.4) and the final buffer solution (0.5 M ammonium acetate in 0.05 M Tris-HCl, pH~7.4). After protein separation, a mixed solution containing the enriched isotopes, 57 Fe, 65 Cu and 67 Zn, was continuously pumped and mixed with the biological species eluting from the column. The analytical signals for Fe ( 56 Fe/ 57 Fe), Cu ( 63 Cu/ 65 Cu) and Zn ( 64 Zn/ 67 Zn) were monitored on a double-focusing inductively coupled plasma mass spectrometry (ICP-MS) instrument operated at medium resolving power (m/Dm~3000). This resolution allowed adequate separation of polyatomic interferences affecting the main isotopes of Fe, Cu and Zn in serum. The conventional abundance vs. time chromatogram obtained was converted to a ''mass flow chromatogram'' and the amount of metal in the different fractions (biomolecules) was obtained by integration of such chromatographic peaks. Total metal contents of Fe, Cu and Zn were also determined by direct isotope dilution analysis (IDA) of the serum samples, diluted 1 : 20, using the same ICP-MS. The method proposed was finally applied to the speciation of serum samples from healthy volunteers and patients on hemodialysis. Some speciation differences were observed between both populations, particularly for Fe speciation, indicating that quantitative speciation studies could be useful for disease diagnostic purposes.

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“…Moving average smoothing was applied next (n~5) and finally the mass flow was computed from each point in the chromatogram using the measured ratio as R m in eqn. (9). Fig.…”

Section: On-line Isotope Dilution Analysis For Fe Cu and Zn In Human ...mentioning

confidence: 99%

Speciation of essential elements in human serum using anion-exchange chromatography coupled to post-column isotope dilution analysis with double focusing ICP-MS

Muñiz

Marchante-Gayón

Alonso

et al. 2001

J. Anal. At. Spectrom.

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“…159 This is offset by a higher background noise, reducing the gain in detection limit to a factor of 2-10. 171,172 4.2.1 Atomic absorption spectrometry (AAS). Post-column spectrophotometric reaction with Pyrocatechol Violet was used for the detection of Al in SE HPLC of an extract of a tea sample.…”

Section: Element-specific Detection In Hplcmentioning

confidence: 99%

“…68,158,162,[171][172][173][174][175][176][177][178][179][180] They can be decreased by the use of an ultrasonic nebulizer 68 or a direct injection nebulizer (in absolute terms). 68,158,162,[171][172][173][174][175][176][177][178][179][180] They can be decreased by the use of an ultrasonic nebulizer 68 or a direct injection nebulizer (in absolute terms).…”

Section: Inductively Coupled Plasma Atomic Emission Spectrometry (Icpmentioning

confidence: 99%

Bio-inorganic speciation analysis by hyphenated techniques

Szpunar

2000

Analyst

279

197

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International audienceThe state-of-the-art of species-selective analysis for trace metals and metalloids in biological materials by chromatographic and electrophoretic separation techniques with element selective detection is critically reviewed. The species of interest include organoarsenic, organoselenium and metal complexes with bioglands such as phytochelatins, metallothioneins, proteins and polysaccharides. The separation mechanisms discussed include size-exclusion, anion- and cation-exchange and reversed-phase HPLC and flatbed and capillary zone electrophoresis. Advantages and limitations of various element selective (e.g. AAS, ICP-AES and ICP-MS) and molecule specific (electrospray MS/MS) detection techniques used on- and off-line are discussed. The applications of coupled techniques to the analysis of biological materials are comprehensively reviewed in tabular form. Attention is paid to the sample preparation and sources of error in bioinorganic speciation analysis

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Section: Introductionmentioning

confidence: 99%

“…Here, we employed native separation using size exclusion chromatography (SEC) hyphenated to ICP-MS for detection of metal cofactors according to molecular weight of the eluting biomolecules. Coupling SEC to elemental detection itself is not a new analytical technique; the first featured examples, reported in the 1980s, used atomic absorption 13 and atomic emission spectrometry 14 to detect trace metals in various biological fluids. Since then, a number of examples of SEC hyphenated to mass specific detection have been used to study trace metals in a variety of physiological and disease states, including rat serum, 15 cancerous human thyroid tissue, 16 murine neurological tissue 17 and platinum-treated human blood products 18 to name just a few.…”

Section: Introductionmentioning

confidence: 99%

Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry

et al. 2013

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Metals often determine the chemical reactivity of the proteins to which they are bound. Each cell in the body tightly maintains a unique metalloproteomic profile, mostly dependent on function. This paper describes an analytical online flow injection quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry (SEC-ICP-MS) method, which was applied to profiling the metal-binding proteins found in primary cultures of neurons and astrocytes. This method can be conducted using similar amounts of sample to those used for Western blotting (20-150 μg protein), and has a turnaround time of <15 minutes. Metalloprotein standards for Fe (as ferritin), Cu and Zn (as superoxide dismutase-1) were used to construct multi-point calibration curves for online quantification of metalloproteins by SEC-ICP-MS. Homogenates of primary neuron and astrocyte cultures were analysed by SEC-ICP-MS. Online quantification by external calibration with metalloprotein standards determined the mass of metal eluting from the column relative to time (as pg s(-1)). Total on-column Fe, Cu and Zn detection limits ranged from 0.825 ± 0.005 ng to 13.6 ± 0.7 pg. Neurons and astrocytes exhibited distinct metalloprotein profiles, featuring both ubiquitous and unique metalloprotein species. Separation and detection by SEC-ICP-MS allows appraisal of these metalloproteins in their native state, and online quantification was achieved using this relatively simple external calibration process.

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On-line sequential detection by inductively coupled plasma atomic emission spectrometry of trace elements after liquid chromatography of biological fluids

Cited by 18 publications

References 0 publications

Speciation of essential elements in human serum using anion-exchange chromatography coupled to post-column isotope dilution analysis with double focusing ICP-MS

Speciation of essential elements in human serum using anion-exchange chromatography coupled to post-column isotope dilution analysis with double focusing ICP-MS

Bio-inorganic speciation analysis by hyphenated techniques

Profiling the iron, copper and zinc content in primary neuron and astrocyte cultures by rapid online quantitative size exclusion chromatography-inductively coupled plasma-mass spectrometry

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