On-Line Tendency Control of Dissolved Oxygen Concentration during Aerobic Fed-Batch Fermentations

Zheng, Rongjian; Pan, Feng

doi:10.3390/app9235232

Cited by 4 publications

(3 citation statements)

References 26 publications

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“…This observation was explained due to limiting solubility of oxygen in water (and fermentation media), medium composition changes and increases in the microorganism concentration [ 16 , 25 ]. However, in many microbial processes, the values of dissolved pO 2 played a decisive role in bacterial metabolism [ 47 ]; an oxygen deficiency will lead to either cessation of aerobic growth or a reduction in substrate uptake efficiency and production of undesirable by-products [ 16 , 48 ]. Subsequently, a constant pO 2 level can be maintained during the fed-batch mode operation to reach a balance between oxygen consumption and supply.…”

Section: Discussionmentioning

confidence: 99%

Scale-Up of Capsular Polysaccharide Production Process by Haemophilus influenzae Type b Using kLa Criterion

2022

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Polyribosyl-ribitol-phosphate (PRP) from Haemophilus influenzae type b (Hib) is an active immunizing molecule used in the production of the vaccine against H. influenzae, and industrial production could contribute to satisfying a world demand especially in developing countries. In this sense, the aim of this study was to establish a scale-up process using the constant oxygen mass transfer coefficient (kLa) such as the criterion for production of PRP in three different sizes of bioreactor systems. Three different kLa values (24, 52 and 80 h−1) were evaluated in which the biological influence in a 1.5 L bioreactor and 52 h−1 was selected to scale-up the production process until a 75 L pilot-scale bioreactor was achieved. Finally, the fed-batch phase was started under a dissolved oxygen concentration (pO2) at 30% of the saturation in the 75 L bioreactor to avoid oxygen limitation; the performance of production presented high efficiency (9.0 g/L DCW-dry cell weight and 1.4 g/L PRP) in comparison with previous scale-up studies. The yields, productivity and kinetic behavior were similar in the three-size bioreactor systems in the batch mode indicating that kLa is possible to use for PRP production at large scales. This process operated under two stages and successfully produced DCW and PRP in the pilot scale and could be beneficial for future bioprocess operations that may lead to higher production and less operative cost.

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Section: Discussionmentioning

confidence: 99%

Scale-Up of Capsular Polysaccharide Production Process by Haemophilus influenzae Type b Using kLa Criterion

2022

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“…One of the most frequently employed methods is the manipulation of the agitation speed to promote the mass transfer of the gas bubbles through the gas-liquid (cell culture medium) interface. The second method involves adjustment of the aeration rate, and the third method is based on altering the partial pressure of oxygen in the gas phase [40]. In this research, dissolved oxygen was controlled by adaptation of agitation speed without pure oxygen feed or changing the rate of aeration because the agitation alone can supply enough dissolved oxygen through this controlled cascade.…”

Section: Effects Of Different Ph-controlled Culture On Vip3a Producti...mentioning

confidence: 99%

Physical Factors Affecting the Scale-Up of Vegetative Insecticidal Protein (Vip3A) Production by Bacillus thuringiensis Bt294

Malairuang,

Nutaratat,

Werapan

et al. 2023

Fermentation

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Vip3A (vegetative insecticidal protein) is a representative member of the Vip3 family, which is widely used for lepidopteran pest control. This Vip3A protein, a non-growth-associated protein, is an effective bioinsecticide against insect pests, but there is relatively little information about its production processes at large scales. Hence, the effects of environmental factors on Vip3A production by Bacillus thuringiensis Bt294 (antifoam agents, shaking speeds, agitation and aeration rates), as well as controlling physical conditions such as the lowest point of dissolved oxygen and controlling of culture pH, were observed in shaking flasks and bioreactors. The results showed that antifoam agents, flask types and shaking speeds had significant effects on Vip3A and biomass production. Cultivation without pH control and DO control in 5 L bioreactors at lower agitation and aeration rates, which was not favorable for biomass production, resulted in a high Vip3A protein production of 5645.67 mg/L. The scale-up studies of the Vip3A protein production in a pilot-scale 750 L bioreactor gave 3750.0 mg/L. Therefore, this study demonstrated the significant effects of agitation, aeration rates and culture pH on Vip3A production by B. thuringiensis Bt294. Balancing of physical conditions was necessary for obtaining the highest yield of Vip3A by slowing down the production rate of biomass. Moreover, this Vip3A protein has high potential as a bioinsecticide for lepidopteran pest control in organic crops. This information will be important for significantly increasing the Vip3A protein concentration by the bacterium and will be useful for field application at a lower cost.

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“…For the bioreactor, a number of control strategies have been applied. For example, DO-stat control strategy [96][97][98][99][100][101] and Extremum seeking control strategy [102][103][104]. DO stat or pH stat control strategies are based on the concept of indirect feedback control.…”

Section: Model-based Controlmentioning

confidence: 99%

Bioprocess Control: Current Progress and Future Perspectives

Rathore

Mishra

Nikita

et al. 2021

Life

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Typical bioprocess comprises of different unit operations wherein a near optimal environment is required for cells to grow, divide, and synthesize the desired product. However, bioprocess control caters to unique challenges that arise due to non-linearity, variability, and complexity of biotech processes. This article presents a review of modern control strategies employed in bioprocessing. Conventional control strategies (open loop, closed loop) along with modern control schemes such as fuzzy logic, model predictive control, adaptive control and neural network-based control are illustrated, and their effectiveness is highlighted. Furthermore, it is elucidated that bioprocess control is more than just automation, and includes aspects such as system architecture, software applications, hardware, and interfaces, all of which are optimized and compiled as per demand. This needs to be accomplished while keeping process requirement, production cost, market value of product, regulatory constraints, and data acquisition requirements in our purview. This article aims to offer an overview of the current best practices in bioprocess control, monitoring, and automation.

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On-Line Tendency Control of Dissolved Oxygen Concentration during Aerobic Fed-Batch Fermentations

Cited by 4 publications

References 26 publications

Scale-Up of Capsular Polysaccharide Production Process by Haemophilus influenzae Type b Using kLa Criterion

Scale-Up of Capsular Polysaccharide Production Process by Haemophilus influenzae Type b Using kLa Criterion

Physical Factors Affecting the Scale-Up of Vegetative Insecticidal Protein (Vip3A) Production by Bacillus thuringiensis Bt294

Bioprocess Control: Current Progress and Future Perspectives

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