On-line two-step stacking in capillary zone electrophoresis for the preconcentration of strychnine and brucine

Yang, Xiu‐Wei; Zhang, Shuaihua; Wang, Juntao; Wang, Chun

doi:10.1016/j.aca.2014.01.027

Cited by 24 publications

(19 citation statements)

References 30 publications

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“…Figure shows the electropherograms for the target analytes by FASS‐MCDS (a) and normal CZE (b) methods. The SEF was calculated by dividing the peak area obtained by regular injection (20 µg/mL, 50 mbar, 6 s) with that gained by double focusing method (5 µg/mL, 50 mbar, 240 s) and multiplying the dilution factor . The SEFs of matrine and oxymatrine were 169 and 218, respectively.…”

Section: Resultsmentioning

confidence: 99%

“…Figure 3 shows the electropherograms for the target analytes Matrine, oxymatrine CE 1.1, 1.0 [28] Matrine, oxymatrine FI-CE a 2.23, 3.11 [29] Matrine, oxymatrine HPLC 2.33, 0.55 [30] Matrine, oxymatrine MLC b 0.5, 1.0 [31] Matrine, oxymatrine FASS-MCDS 0.01, 0.02 This method a FI-CE: CE combination with FI analysis; b MLC: micellar liquid chromatography by FASS-MCDS (a) and normal CZE (b) methods. The SEF was calculated by dividing the peak area obtained by regular injection (20 µg/mL, 50 mbar, 6 s) with that gained by double focusing method (5 µg/mL, 50 mbar, 240 s) and multiplying the dilution factor [27]. The SEFs of matrine and oxymatrine were 169 and 218, respectively.…”

Section: Methods Validationmentioning

confidence: 99%

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On‐line synergistic stacking in capillary zone electrophoresis featuring field‐amplified sample stacking and micelle to cyclodextrin stacking in the determination of two alkaloids in complicated matrix samples

Chu

Liu

Jiang

et al. 2019

J of Separation Science

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A novel on‐line synergistic proconcentration strategy coupling field‐amplified sample stacking and micelle to cyclodextrin stacking for cationic analytes in capillary zone electrophoresis has been proposed and applied for the separation and determination of two alkaloids, matrine, and oxymatrine in complicated matrix samples. The approach was performed by the long injection of sample in a low‐conductivity sodium dodecyl benzene sulfonate solution followed by the injection of hydroxypropyl‐β‐cyclodextrin solution in higher conductivity. The stacking mechanism of this method has been expounded and parameters affecting stacking effect have been optimized in our study. Under the optimum experimental conditions, 169‐ and 218‐fold sensitivity improvements were achieved for matrine and oxymatrine when compared with normal injection. Analytical indicators including linearity, limits of detection, and reproducibility (intra‐ and inter‐day relative standard deviations) were evaluated. Moreover, sample matrix effect was studied using compound flavescent sophora and salicylic acid powder and spiked urine samples. The developed method is an attempt for the combination of micelle to cyclodextrin stacking with other stacking methods. It could be a good alternative choice for the determination of alkaloids in a complex sample matrix.

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Section: Resultsmentioning

confidence: 99%

Section: Methods Validationmentioning

confidence: 99%

On‐line synergistic stacking in capillary zone electrophoresis featuring field‐amplified sample stacking and micelle to cyclodextrin stacking in the determination of two alkaloids in complicated matrix samples

Chu

Liu

Jiang

et al. 2019

J of Separation Science

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show abstract

“…A simple and convenient two‐step stacking method combining sweeping with MSS was described by Yang et al. for simultaneous online preconcentration and CZE determination of strychnine and brucine. The separation was performed in 75 mM phosphate buffer at pH 2.5 in 30% methanol and compared with normal CZE injection, 51‐ and 38‐fold improvement in concentration sensitivity was achieved for strychnine and brucine, respectively.…”

Section: Micellar Methodsmentioning

confidence: 99%

Contemporary sample stacking in analytical electrophoresis

et al. 2014

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This contribution is a methodological review of the publications about the topic from the last 2 years. Therefore, it is primarily organized according to the methods and procedures used in surveyed papers and the origin and type of sample and specification of analytes form the secondary structure. The introductory part about navigation in the architecture of stacking brings a brief characterization of the various stacking methods, with the description of mutual links to each other and important differences among them. The main body of the article brings a survey of publications organized according to main principles of stacking and then according to the origin and type of the sample. Provided that the paper cited gave explicitly the relevant data, information about the BGE(s) used, procedure, detector employed, and reached LOD and/or concentration effect is given. The papers where the procedure used is a combination of diverse fragments and parts of various stacking techniques are mentioned in a special section on combined techniques. The concluding remarks in the final part of the review evaluate present state of art and the trends of sample stacking in CE.

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“…On-line preconcentration techniques in advance of MEKC can stack large sample ions in a narrow band by the manipulation of electrophoretic velocities, and several on-line preconcentration methods have been developed to increase sensitivity. Stacking strategies include micelle to solvent stacking [16][17][18][19], analyte focusing by micelle collapse-micellar electrokinetic chromatography (AFMC-MEKC) [20][21][22][23][24][25] and so on. Of these techniques, analyte focusing by micelle collapse-micellar electrokinetic chromatography (AFMC-MEKC) was introduced by Quirino et al [26] and enhanced the sensitivity by 160-to 200-fold compared to the MEKC mode.…”

Section: Introductionmentioning

confidence: 99%

Determining ultraviolet absorbents in sunscreen products by combining direct injection with micelle collapse on-line preconcentration capillary electrophoresis

Hsiao

Jiang

Feng

et al. 2015

Journal of Chromatography A

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On-line two-step stacking in capillary zone electrophoresis for the preconcentration of strychnine and brucine

Cited by 24 publications

References 30 publications

On‐line synergistic stacking in capillary zone electrophoresis featuring field‐amplified sample stacking and micelle to cyclodextrin stacking in the determination of two alkaloids in complicated matrix samples

On‐line synergistic stacking in capillary zone electrophoresis featuring field‐amplified sample stacking and micelle to cyclodextrin stacking in the determination of two alkaloids in complicated matrix samples

Contemporary sample stacking in analytical electrophoresis

Determining ultraviolet absorbents in sunscreen products by combining direct injection with micelle collapse on-line preconcentration capillary electrophoresis

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