On-line water removal during enzymatic triacylglycerol synthesis by means of pervaporation

Padt, A. van der; Sewalt, J.J.W.; Riet, K. van ’t

doi:10.1016/0376-7388(93)85144-l

Cited by 54 publications

(12 citation statements)

References 21 publications

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“…in such reaction systems is a critical parameter since the synthetic activity of lipases is often strongly dependent on water activity (Valivety et ul., 1992a and b;Oladepo ~t al., 1994). A number of techniques have been described in the literature for water removal (Goderis et al, 1987;Miller et a/., 1988;Ergan et a/., 1990;Van der Padt et a]., 1993;Gubicza and Szakacsschmidt, 1995) and water activity control (Kvittingen e f al., 1992, Wehtje et d . , 1993, Yang and Robb, 1993Wehtje et nl., 1997).…”

Section: Introductionmentioning

confidence: 97%

Dependence of the Synthetic Activity of Nine Immobilized Lipases on Water Activity

Köller

Aris

Ujang

et al. 2001

Biocatalysis and Biotransformation

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The advantages of carrying out enzymatic syntheses in organic media with water activity, a,, control are now well recognized and a number of techniques for accomplishing this have been described in the literature. Recently, many of these ideas have been extended to allow the continuous control of water activity in reactor designs suitable for scaleup. For the proper design and operation of such devices, it is essential to have some knowledge regarding the water activity behavior of enzyme preparations suitable for use with them. This article describes the a," profiles of nine different lipases, adsorbed on a microporous polypropylene support, used for the synthesis of dodecyl decanoate from a solution of the corresponding acid and alcohol in hexane.

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Section: Introductionmentioning

confidence: 97%

Dependence of the Synthetic Activity of Nine Immobilized Lipases on Water Activity

Köller

Aris

Ujang

et al. 2001

Biocatalysis and Biotransformation

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“…Pervaporation is defined as the transport of liquid through a nonporous membrane with simultaneous evaporation of the permeates. Since pervaporation was first used for enzymatic triacylglycerol synthesis by Van der Padt et al [9], it has been applied to lipase-catalyzed esterification reactions to remove water formed during the reaction. Esterifications of fatty acid with ethanol [8], n-butanol [ iso-amyl alcohol [11], and fusel oil [12] in the presence of lipases were successfully carried out with in situ water removal by pervaporation.…”

Section: Introductionmentioning

confidence: 99%

Lipase-catalyzed enantioselective esterification of racemic ibuprofen coupled with pervaporation

Won

Hong

Kim

et al. 2006

Process Biochemistry

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“…Different means of controling water in enzymatic esterification have been reported, including the use of a pair of solid salt hydrates (Kvittingen et al, 1992), evaporation (Ergan et al, 1990), and pervaporation (Kwon et al, 1995;van der Padt et al, 1993). Adsorption has also been considered.…”

Section: Introductionmentioning

confidence: 99%

Adsorptive control of water in esterification with immobilized enzymes: I. Batch reactor behavior

Mensah

Gainer

Carta

1998

Biotechnol. Bioeng.

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Reducing the influence of an undesired product in an enzymatic reaction could have a significant impact on the productivity of such systems. Here, we focus on the removal of water formed during an enzymatic esterification in a batch reactor. A commercial immobilized lipase preparation, known as Lipozyme, is used as the biocatalyst and propionic acid and isoamyl alcohol dissolved in hexane are the substrates. In this system, the water formed will partition between the catalyst and the medium. As the more polar reactants are converted into the less polar ester product, the water is partitioned more towards the biocatalyst and the accumulation of water eventually causes lower reaction rates. Addition of a strong‐acid cation exchange resin in sodium form is found to control the water accumulation on the biocatalyst without stripping the essential water needed for the enzyme to function and substantial improvements in conversion are achieved. A mathematical model is developed to describe the batch reaction behavior with and without added absorbent, which successfully predicts the behavior of water and its effects. © 1998 John Wiley & Sons, Inc. Biotechnol Bioeng 60: 434–444, 1998.

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On-line water removal during enzymatic triacylglycerol synthesis by means of pervaporation

Cited by 54 publications

References 21 publications

Dependence of the Synthetic Activity of Nine Immobilized Lipases on Water Activity

Dependence of the Synthetic Activity of Nine Immobilized Lipases on Water Activity

Lipase-catalyzed enantioselective esterification of racemic ibuprofen coupled with pervaporation

Adsorptive control of water in esterification with immobilized enzymes: I. Batch reactor behavior

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