On measurements and their quality: Paper 3: Post hoc pooling and errors of discreteness

Beckstead, Jason W.

doi:10.1016/j.ijnurstu.2013.06.003

Cited by 4 publications

(1 citation statement)

References 32 publications

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“…They likely transformed their continuous agromorphological traits into new discrete variables. Such a transformation leads to loss of information and unpredictable bias (Beckstead, 2014;MacCallum et al, 2002;Irwin & McClelland, 2003;Royston et al, 2006;Young, 2016).…”

Section: Introductionmentioning

confidence: 99%

Appropriate statistical methods for analysis of safflower genetic diversity using agglomerative hierarchical cluster analysis through combination of phenotypic traits and molecular markers

Houmanat

Douaik²,

Charafi

et al. 2021

Crop Science

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Combining phenotypic and genotypic germplasm characterization is a key to efficient and successful safflower (Carthamus tinctorius L.) breeding program by identifying valuable and confirmed parents. This study aimed to investigate and use appropriate statistical methods for such a characterization, and to identify potential genetic pools in safflower germplasm that may be useful for breeding program implementation. The genetic diversity of 45 accessions from different countries, provided by the USDA-ARS, was assessed during two cropping seasons, using agromorphological traits and inter simple sequence repeat (ISSR) molecular markers. Agglomerative hierarchical cluster analysis (AHCA) was used with appropriate similarity distances, and Ward and unweighted pair group method with arithmetic mean (UPGMA) linkages. Agreement between distance-linkage combinations was evaluated using cophenetic correlation, Mantel test, Fisher exact test, Cramer's V, overall accuracy, and Cohen's κ. Both agromorphological phenotyping and molecular genotyping revealed significant genetic diversity. Ward linkage was better than UPGMA, using simple matching distance for molecular markers and Gower distance for phenotypic traits as well as for combined phenotypic traits and molecular markers. It delineated the studied accessions into four main clusters. Some accessions showed desirable profiles that can be used in future breeding programs. This is the first report of a series of appropriate statistical methods that can be used for assessing genetic diversity in safflower, combining phenotypic traits and molecular markers, and thus identifying relevant genetic pools for breeding program.

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