On-site analysis of acetylcholinesterase and butyrylcholinesterase activity with the ChE check mobile test kit—Determination of reference values and their relevance for diagnosis of exposure to organophosphorus compounds

Worek, Franz; Schilha, Martina; Neumaier, Katharina; Aurbek, Nadine; Wille, Timo; Thiermann, Horst; Kehe, Kai

doi:10.1016/j.toxlet.2016.03.007

Cited by 29 publications

(20 citation statements)

References 27 publications

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“…After the re-start of the obidoxime infusion on day 9, reactivatability was at a level of ~ 200 mU/µmol Hb. This level is clearly below the normal range of 531-796 mU/µmol Hb and indicates aging (Worek et al 2016b). The aging halflife of diethyl-phosphorylated AChE is about 30 h in the absence of poison and oximes (Worek et al 1997).…”

Section: Discussionmentioning

confidence: 86%

“…The current AChE and BChE activity, the AChE reactivatability, and the presence of inhibitory material in the patient plasma were assessed. Among the aforementioned parameters, four independent analyses are: (1) the in vivo activity of patient´s erythrocyte-AChE; (2) the reactivatability (incubation of diluted patient blood at 37 °C for 30 min with a supratherapeutic oxime concentration of 100 µM obidoxime for a defined interval to achieve a maximum possible AChE reactivation by treatment, followed by determination of AChE activity); (3) the in vivo activity of patient plasma BChE activity and IV) the inhibitory activity of patient plasma (incubation of patient plasma at 37 °C for 60 min with AChE control, followed by determination of AChE activity) (Thiermann et al 1997;Worek et al 2016b).…”

Section: Methodsmentioning

confidence: 99%

“…There are high inter-and intra-individual variations of BChE activity which can be affected by a variety of parameters, e.g. drugs or nutrition (Worek et al 2016b). In contrast, the single gene-encoded AChE is expressed on red blood cell (RBC) membranes showing comparable properties with AChE in other tissues within an individual.…”

Section: Introductionmentioning

confidence: 99%

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A case report of cholinesterase inhibitor poisoning: cholinesterase activities and analytical methods for diagnosis and clinical decision making

et al. 2020

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Suicidal ingestion of organophosphorus (OP) or carbamate (CM) compounds challenges health care systems worldwide, particularly in Southeast Asia. The diagnosis and treatment of OP or CM poisoning is traditionally based on the clinical appearance of the typical cholinergic toxidrome, e.g. miosis, salivation and bradycardia. Yet, clinical signs might be inconclusive or even misleading. A current case report highlights the importance of enzymatic assays to provide rapid information and support clinicians in diagnosis and rational clinical decision making. Furthermore, the differentiation between OP and CM poisoning seems important, as an oxime therapy will most probably not provide benefit in CM poisoning, but-as every pharmaceutical product-it might result in adverse effects. The early identification of the causing agent and the amount taken up in the body are helpful in planning of the therapeutic regimen including experimental strategies, e.g. the use of human blood products to facilitate scavenging of the toxic agent. Furthermore, the analysis of biotransformation products and antidote levels provides additional insights into the pathophysiology of OP or CM poisoning. In conclusion, cholinesterase activities and modern analytical methods help to provide a more effective treatment and a thorough understanding of individual cases of OP or CM poisoning.

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Section: Discussionmentioning

confidence: 86%

Section: Methodsmentioning

confidence: 99%

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A case report of cholinesterase inhibitor poisoning: cholinesterase activities and analytical methods for diagnosis and clinical decision making

et al. 2020

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“…This reliably and rapidly measures AChE and BChE activity and is validated against standard reference methods [1]. In cooperation with medical scientists of the German Armed Forces, Securetec developed the compact "ChE check mobile," another portable system for measuring AChE and BChE [9]. The study aims to evaluate the accuracy of ChE check mobile compared to the standard laboratory reference method and Test-mate ChE.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of the accuracy of “ChE check mobile” in measurement of acetylcholinesterase in pesticide poisoning

Shihana

Worek²,

Dassanayake

et al. 2018

Clinical Toxicology

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Background: Acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) are used in clinical management to confirm the diagnosis and indicate the severity of organophosphorus and carbamate poisoning. ChE check mobile is a new portable cholinesterase testing system developed in Germany. The study aims to evaluate the accuracy of ChE check mobile compared to the standard reference method and Test-mate ChE system. Methods: Patients with organophosphorus and carbamate poisoning were recruited from two general hospitals in Sri Lanka between September 2013 and November 2014. The AChE was measured using the three methods. Results: Blood samples were collected from 185 self-poisoned patients (170 organophosphorus and 15 carbamate) and 20 normal individuals. ChE check mobile correlated well with spectrophotometer readings (Pearson's correlation coefficient 0.87) but gave higher values (Mean bias for AChE: þ6.55 (95% CI: À11 to 24) U/g Hb). A similar positive bias from Test-mate results was also observed. Applying a correction factor derived from the volunteer samples (dividing by 1.353) greatly improved agreement in pesticide poisoned patients. Conclusions: ChE check mobile system allowed for rapid determination of AChE activity but gave somewhat higher AChE compared to other methods. Applying a correction factor of 1.353 provide a good agreement to both reference and Test-mate ChE machine in this setting.

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“… 40 , 41 X-ray structures have indicated that the although the butyrylcholinesterase (BChE) and AChE structures are similar, multiple structural discrepancies in the active-site gorges and the active sites have been observed. 42 , 43 BChE has of toxicological and pharmacological importance because it scavenges ChEIs, including potent organophosphorus nerve factors, before they bind synapses and hydrolyzes ester-containing drugs 44 . BChE is also important for drug metabolism such as cocaine.…”

Section: Introductionmentioning

confidence: 99%

Synthesis and biological evaluation of aminomethyl and alkoxymethyl derivatives as carbonic anhydrase, acetylcholinesterase and butyrylcholinesterase inhibitors

Gülçın

Abbasova

Taslimi

et al. 2017

Journal of Enzyme Inhibition and Medicinal Chemistry

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Compounds containing nitrogen and sulfur atoms can be widely used in various fields such as industry, medicine, biotechnology and chemical technology. Therefore, the reactions of aminomethylation and alkoxymethylation of mercaptobenzothiazole, mercaptobenzoxazole and 2-aminothiazole were developed. Additionally, the alkoxymethyl derivatives of mercaptobenzoxazole and 2-aminothiazole were synthesized by a reaction with hemiformals, which are prepared by the reaction of alcohols and formaldehyde. In this study, the inhibitory effects of these molecules were investigated against acetylcholinesterase (AChE), butyrylcholinesterase (BChE) enzymes and carbonic anhydrase I, and II isoenzymes (hCA I and II). Both hCA isoenzymes were significantly inhibited by the recently synthesized molecules, with K values in the range of 58-157 nM for hCA I, and 81-215 nM for hCA II. Additionally, the K parameters of these molecules for BChE and AChE were calculated in the ranges 23-88 and 18-78 nM, respectively.

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On-site analysis of acetylcholinesterase and butyrylcholinesterase activity with the ChE check mobile test kit—Determination of reference values and their relevance for diagnosis of exposure to organophosphorus compounds

Cited by 29 publications

References 27 publications

A case report of cholinesterase inhibitor poisoning: cholinesterase activities and analytical methods for diagnosis and clinical decision making

A case report of cholinesterase inhibitor poisoning: cholinesterase activities and analytical methods for diagnosis and clinical decision making

Evaluation of the accuracy of “ChE check mobile” in measurement of acetylcholinesterase in pesticide poisoning

Synthesis and biological evaluation of aminomethyl and alkoxymethyl derivatives as carbonic anhydrase, acetylcholinesterase and butyrylcholinesterase inhibitors

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