On-site blood culture incubation shortens the time to knowledge of positivity and microbiological results in septic patients

Schwarzenbacher, Julika; Kuhn, Sven–Olaf; Vollmer, Marcus; Scheer, Christian; Fuchs, Christian; Rehberg, Sebastian; Balau, Veronika; Hahnenkamp, Klaus; Bohnert, Jürgen A.; Gründling, Matthias

doi:10.1371/journal.pone.0225999

Cited by 18 publications

(26 citation statements)

References 41 publications

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“…Our research suggested that satellite blood culture signi cantly reduces turnaround time including TI and TK(P < 0.001), which is similar with the previous studies [8,9]. In our study, compared with medical microbiology laboratory blood culture group, TI was reduced by 3.02 h and TK was reduced by 9.76 h in satellite blood culture group.…”

Section: Discussionsupporting

confidence: 89%

“…However, when it comes to TA, the study didn't demonstrate that satellite blood culture is better than medical microbiology laboratory blood culture (p = 0.056),although the median time from specimen collection to adjust antibiotic was reduced by 23.08 h in satellite blood culture group. It is contrary to Schwarzenbacher's ndings [9]. Small sample size maybe the main reason for it.…”

Section: Discussioncontrasting

confidence: 70%

“…In our study, compared with medical microbiology laboratory blood culture group, TI was reduced by 3.02 h and TK was reduced by 9.76 h in satellite blood culture group. It indicated that when TI was only 3 hours earlier, TK was roughly 10 hours earlier, which is related to work ow in the lab [10].Schwarzenbacher et al controled the incubation time within 1 h in satellite blood culture group, even if in the medical microbiology laboratory blood culture group, the incubation time were not more than 8h [9].which is better than our data. Maybe it because we didn't train the staffs to get blood culture incubated as soon as possible.…”

Section: Discussioncontrasting

confidence: 57%

“…Satellite blood culture is able to signi cantly reduces time to obtain test results and speeds up antibiotic adjustment [8,9]. It is useful for the early diagnosis and treatment of sepsis.…”

Section: Introductionmentioning

confidence: 99%

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The Clinical Value of Satellite Blood Culture in the Early Diagnosis of Sepsis

Guo

Wang

et al. 2020

Preprint

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Background: Early antibiotic treatment is a key component of therapy for sepsis. However, current blood culture can’t fulfill it. The aim of this study was to assess whether satellite blood cultures (immediate incubation of blood cultures in the ICU) can improve turnaround times, antibiotic switching and patient prognosis. Methods: This a observational single-center study, satellite blood culture and medical microbiology laboratory blood culture were performed separately in two groups. septic patients are collected from department of critical care medicine, Henan Provincial People`s Hospital from February 5,2018, to January 19, 2019.All septic patients are older than 18 and diagnosed sepsis according sepsis-3 criteria, who have been stayed hospital stay longer than 48 hour admitted to ICU. Results: In total, we analyzed 299 blood culture sets from 73 ICU patients, including:149 from the medical microbiology laboratory blood culture group and 150 from the satellite blood culture group. There is no significant difference between the two group in positive rate. Comparing to laboratory blood culture group, the median time from specimen collection until incubation as reduced by 3.02h in satellite blood culture group (P < 0.001); the median time from specimen collection to notify blood culture positivity to the clinicians was reduced by 9.76h in satellite blood culture group(P <0.001).The median time was 54.05h in medical microbiology laboratory blood culture group and 30.97h in satellite blood culture group until the first change of antibiotic therapy regimen (P = 0.056).There was no difference in duration of ICU stay、duration of hospital stay、medical cost between the two group. Conclusion: We have found that satellite blood culture may reduced turnaround times and it may speed up antibiotic switching and benefit on patients outcomes.

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Section: Discussionsupporting

confidence: 89%

Section: Discussioncontrasting

confidence: 70%

Section: Discussioncontrasting

confidence: 57%

Section: Introductionmentioning

confidence: 99%

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The Clinical Value of Satellite Blood Culture in the Early Diagnosis of Sepsis

Guo

Wang

et al. 2020

Preprint

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“…Between 30 and 50% of patients with suspected sepsis or shock have positive blood cultures [ 14 ] and blood stream infections are associated with 14–37% mortality [ 15 , 16 ]. Schwarzenbacher et al recently commented that only 20% of laboratories in Europe offer 24 h microbiology services, thereby adding additional challenges for the timely reporting of results (COVID-19 or other pathogens) [ 17 ]. Idelevich et al reported only 13% of laboratories from 25 European countries provide 24 h microbiology service [ 18 ].…”

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confidence: 99%

Clinical Microbiology Laboratories and COVID-19: The Calm Before the Storm

Blondeau

2020

Future Microbiol.

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Analysis of Blood Culture Collection and Laboratory Processing Practices in Israel

et al. 2022

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ImportanceBlood culturing is a critical diagnostic procedure affecting patient outcomes and antibiotic stewardship. Although there are standards for blood culturing, the process is not often measured.ObjectivesTo evaluate processes related to the diagnosis of bloodstream infection and compare them with best practices.Design, Setting, and ParticipantsA quality improvement study using laboratory data from January 1 to June 30, 2019, was conducted in 28 (96.6%) Israeli acute care hospitals. All blood cultures (BCs) performed on samples from adults and children in a period of 147 hospital-months were analyzed. Data analysis was performed from April 12, 2021, to September 9, 2022.Main Outcomes and MeasuresTrue pathogen detection rate, contamination rate, proportion of adults with blood cultures performed, proportion of adult culturing episodes with only 1 set or bottle used, and median time of steps from sample collection to pathogen identification.ResultsThe data set consisted of 348 987 BC bottles. Bloodstream infection was detected in a median of 6.7% (IQR, 5.8%-8.2%) of adult culturing episodes and 1.1% (IQR, 0.7%-1.9%) of pediatric episodes. Eleven of 27 hospitals (40.7%) with adult patients met the standard of a contamination rate of less than 3% and only 2 hospitals (7.4%) met the more stringent standard of less than or equal to 1% contamination rate. The percentage of adults with blood cultures ranged from 2.7% to 29.0% (mean [SD], 15.7% [6.0%]). There was an association between sampling rate and pathogen detection until BCs were performed in 17% of adult admissions. The percentage of solitary BCs ranged from 47.8% to 94.4%. An estimated 1745 of 7436 (23.5%) adult bloodstream infections went undetected because solitary BCs were performed, anaerobic bottles were not used, or BCs were not performed. Median processing time was 51.2 (IQR, 33.9-78.0) hours, 3 times the optimal time: 4.4 (IQR, 1.7-12.5) hours for the preanalytical stage, 15.9 (IQR, 10.2-23.6) hours from incubation to growth detection, 4.5 (IQR, 1.5-10.7) hours from detection to Gram stain, and 30.9 (IQR, 22.0-41.9) hours from detection to isolate identification. An 8.6-hour delay was related to off-hours operating of laboratories.Conclusions and RelevanceThe findings of this study suggest that the multistep process of blood culturing is not managed comprehensively in Israel, leading to poor clinical practices and delayed results.

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On-site blood culture incubation shortens the time to knowledge of positivity and microbiological results in septic patients

Abstract: Onsite blood culture incubation shortens the time to knowledge of positivity and microbiological results in septic patients. PLoS ONE 14(12): e0225999.

Cited by 18 publications

References 41 publications

The Clinical Value of Satellite Blood Culture in the Early Diagnosis of Sepsis

The Clinical Value of Satellite Blood Culture in the Early Diagnosis of Sepsis

Clinical Microbiology Laboratories and COVID-19: The Calm Before the Storm

Analysis of Blood Culture Collection and Laboratory Processing Practices in Israel

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