On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor

Moon, Jeong Hee; Byun, Jaechul; Kim, Hongki; Lim, Eun‐Kyung; Jeong, Jinyoung; Jung, Juyeon; Kang, Taejoon

doi:10.3390/s18020598

Cited by 36 publications

(15 citation statements)

References 32 publications

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“…Therefore, their use in field conditions is not possible. Recently, a palm sized SPR device has been developed [ 69 ] for the on-site detection of aflatoxin B1 in infected grains. Many other researchers have also used these kinds of mini SPR devices for the detection of different chemical and biological species [ 70 ].…”

Section: Quantification/detection Of Aflatoxinmentioning

confidence: 99%

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Shabeer

Asad

Jamal

et al. 2022

Toxins

121

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Aflatoxin, a type of mycotoxin, is mostly produced by Aspergillus flavus and Aspergillus parasiticus. It is responsible for the loss of billions of dollars to the world economy, by contaminating different crops such as cotton, groundnut, maize, and chilies, and causing immense effects on the health of humans and animals. More than eighteen different types of aflatoxins have been reported to date, and among them, aflatoxins B1, B2, G1, and G2 are the most prevalent and lethal. Early detection of fungal infection plays a key role in the control of aflatoxin contamination. Therefore, different methods, including culture, chromatographic techniques, and molecular assays, are used to determine aflatoxin contamination in crops and food products. Many countries have set a maximum limit of aflatoxin contamination (2–20 ppb) in their food and agriculture commodities for human or animal consumption, and the use of different methods to combat this menace is essential. Fungal infection mostly takes place during the pre- and post-harvest stage of crops, and most of the methods to control aflatoxin are employed for the latter phase. Studies have shown that if correct measures are adopted during the crop development phase, aflatoxin contamination can be reduced by a significant level. Currently, the use of bio-pesticides is the intervention employed in many countries, whereby atoxigenic strains competitively reduce the burden of toxigenic strains in the field, thereby helping to mitigate this problem. This updated review on aflatoxins sheds light on the sources of contamination, and the on occurrence, impact, detection techniques, and management strategies, with a special emphasis on bio-pesticides to control aflatoxins.

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Section: Quantification/detection Of Aflatoxinmentioning

confidence: 99%

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Shabeer

Asad

Jamal

et al. 2022

Toxins

121

View full text Add to dashboard Cite

show abstract

“…Recently, Moon et al designed the SPR portable sensor for on-site detection of AFB 1 in grains (Moon et al 2018). In this work, SPR Au chip treated with cysteine-protein G to orient directionally AFB 1 antibodies on the surface and the modified chip has been conjugated with AFB 1 -BSA into the flowed system continuous to AFB 1 detection in the range of 16-200 ppb.…”

Section: -D Optical Biosensormentioning

confidence: 99%

Recent advances in nanomaterials integrated immunosensors for food toxin detection

2021

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For the management and prevention of many chronic and acute diseases, the rapid quantification of toxicity in food and feed products have become a significant concern. Technology advancements in the area of biosensors, bioelectronics, miniaturization techniques, and microfluidics have shown a significant impact than conventional methods which have given a boost to improve the sensing performance towards food analyte detection. In this article, recent literature of Aflatoxin B 1 (AFB 1 ), worldwide permissible limits, major outbreaks and severe impact on healthy life have been discussed. An improvement achieved in detection range, limit of detection, shelf-life of the biosensor by integrated dimensional nanomaterials such as zero-dimension, one-dimension and two-dimension for AFB 1 detection using electrical and optical transduction mechanism has been summarized. A critical overview of the latest trends using paper-based and micro-spotted array integrated with the anisotropic shape of nanomaterials, portable microfluidic devices have also been described together with future perspectives for further advancements.

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“…However, these analytical techniques often require expensive and bulky instruments and sophisticated laboratory environment. Thus, several biosensors have been developed, such as immunoassay [ 8 , 9 , 10 , 11 ], surface plasmon resonance sensor [ 12 ], electrochemical sensor [ 13 , 14 ] and surface-enhanced Raman spectroscopic (SERS) sensor [ 15 ], fluorescence sensor [ 16 , 17 ], chemiluminescence (CL) sensor [ 18 , 19 , 20 , 21 ], imprinted polymer membrane sensor [ 22 ], and microcantilever sensor [ 23 ]. Compared to other sensor techniques, microcantilever is an ultrasensitive and rapid MEMS device for biochemical sensing.…”

Section: Introductionmentioning

confidence: 99%

A Flexible PI/Si/SiO2 Piezoresistive Microcantilever for Trace-Level Detection of Aflatoxin B1

Tian

Liu

Wang

et al. 2021

Sensors

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In this paper, a polyimide (PI)/Si/SiO2-based piezoresistive microcantilever biosensor was developed to achieve a trace level detection for aflatoxin B1. To take advantage of both the high piezoresistance coefficient of single-crystal silicon and the small spring constant of PI, the flexible piezoresistive microcantilever was designed using the buried oxide (BOX) layer of a silicon-on-insulator (SOI) wafer as a bottom passivation layer, the topmost single-crystal silicon layer as a piezoresistor layer, and a thin PI film as a top passivation layer. To obtain higher sensitivity and output voltage stability, four identical piezoresistors, two of which were located in the substrate and two integrated in the microcantilevers, were composed of a quarter-bridge configuration wheatstone bridge. The fabricated PI/Si/SiO2 microcantilever showed good mechanical properties with a spring constant of 21.31 nN/μm and a deflection sensitivity of 3.54 × 10−7 nm−1. The microcantilever biosensor also showed a stable voltage output in the Phosphate Buffered Saline (PBS) buffer with a fluctuation less than 1 μV @ 3 V. By functionalizing anti-aflatoxin B1 on the sensing piezoresistive microcantilever with a biotin avidin system (BAS), a linear aflatoxin B1 detection concentration resulting from 1 ng/mL to 100 ng/mL was obtained, and the toxic molecule detection also showed good specificity. The experimental results indicate that the PI/Si/SiO2 flexible piezoresistive microcantilever biosensor has excellent abilities in trace-level and specific detections of aflatoxin B1 and other biomolecules.

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On-Site Detection of Aflatoxin B1 in Grains by a Palm-Sized Surface Plasmon Resonance Sensor

Cited by 36 publications

References 32 publications

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Aflatoxin Contamination, Its Impact and Management Strategies: An Updated Review

Recent advances in nanomaterials integrated immunosensors for food toxin detection

A Flexible PI/Si/SiO2 Piezoresistive Microcantilever for Trace-Level Detection of Aflatoxin B1

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