On-site enrichment and detection of live Salmonella typhimurium using a bioluminescence sensor coupled with a hyperbranched aptamer probe-labelled stir-bars array

Wang, Ming; Cao, Cong; Bi, Wenchao; Lin, Jianyuan; Tan, Lei; Gan, Ning

doi:10.1016/j.snb.2022.131862

Cited by 14 publications

(3 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The combination of click chemistry and bioluminescence can greatly increase the density and detection sensitivity of aptamer labeling, and further improve the enrichment efficiency. Among them, Wang et al 35 developed a hand-held agitator array modified with hyperbranched aptamer probes (HAPs) for the specific adsorption extraction of Salmonella typhimurium ( S. T. ) as a model (Fig. 7).…”

Section: Modern Bioluminescence Methods Combined With Other Methods T...mentioning

confidence: 99%

Application of ATP-based bioluminescence technology in bacterial detection: a review

Zhao

Guo

et al. 2023

Analyst

View full text Add to dashboard Cite

show abstract

Section: Modern Bioluminescence Methods Combined With Other Methods T...mentioning

confidence: 99%

Application of ATP-based bioluminescence technology in bacterial detection: a review

Zhao

Guo

et al. 2023

Analyst

View full text Add to dashboard Cite

show abstract

“…Several studies have demonstrated the effectiveness of ATP sensors in detecting dangerous bacteria. For instance, researchers have developed ATP-based assays for the detection of foodborne pathogens, including Salmonella species , Escherichia coli , and Listeria monocytogenes . These assays showed high sensitivity and specificity, with detection limits as low as 10–100 colony-forming units per milliliter (CFU/mL).…”

Section: Commercially Available Biosensor Technologies For the Detect...mentioning

confidence: 99%

Emerging Biomimetic Sensor Technologies for the Detection of Pathogenic Bacteria: A Commercial Viability Study

Frigoli,

Lowdon,

Caldara

et al. 2024

ACS Omega

View full text Add to dashboard Cite

Ensuring a rapid and accurate identification of harmful bacteria is crucial in various fields including environmental monitoring, food safety, and clinical diagnostics. Conventional detection methods often suffer from limitations such as long analysis time, complexity, and the need for qualified personnel. Therefore, a lot of research effort is devoted to developing technologies with the potential to revolutionize the detection of pathogenic bacteria by offering rapid, sensitive, and user-friendly platforms for point-ofcare analysis. In this light, biosensors have gained significant commercial attention in recent years due to their simplicity, portability, and rapid analysis capabilities. The purpose of this review is to identify a trend by analyzing which biosensor technologies have become commercially successful in the field of bacteria detection. Moreover, we highlight the characteristics that a biosensor must possess to finally arrive in the market and therefore in the hands of the end-user, and we present critical examples of the market applications of various technologies. The aim is to investigate the reason why certain technologies have achieved commercial success and extrapolate these trends to the future economic viability of a new subfield in the world of biosensing: the development of biomimetic sensor platforms. Therefore, an overview of recent advances in the field of biomimetic bacteria detection will be presented, after which the challenges that need to be addressed in the coming years to improve market penetration will be critically evaluated. We will zoom into the current shortcomings of biomimetic sensors based on imprinting technology and aptamers and try to come up with a recommendation for further development based on the trends observed from previous commercial success stories in biosensing.

show abstract

“…Additionally shown is the subsequent analytical stage during which the binding of E. coli UspA2 to the aptasensor occurs (Figure 5C), followed by electrochemical detection of the binding event (Figure 5D). Recent studies on the development of PoC platforms towards the detection of bacterial pathogens using aptamers have been reported, including Shigella flexneri (K d = 144 to 329 nM) [46], Staphylococcus aureus (K d = 16.5 nM and 14.47 nM) [47], E. coli (K d = 15.9 nM) [48], Helicobacter pylori (K d = 19.3 nM) [49], Yersinia enterocolitica (K d = 11 nM) [16] and Salmonella typhimurium for which a multi-aptamer probe was reported (K d = 3.09 nM) [50]. The binding affinity of Apt1_RC compares favorably with the binding affinity of other contemporary aptamer candidates selected for other bacterial pathogens.…”

Section: Assembly Of the Aptasensormentioning

confidence: 99%

Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2

et al. 2023

View full text Add to dashboard Cite

Innovative point-of-care (PoC) diagnostic platforms are desirable to surpass the deficiencies of conventional laboratory diagnostic methods for bacterial infections and to tackle the growing antimicrobial resistance crisis. In this study, a workflow was implemented, comprising the identification of new aptamers with high affinity for the ubiquitous surface protein A2 (UspA2) of the bacterial pathogen Moraxella catarrhalis and the development of an electrochemical biosensor functionalized with the best-performing aptamer as a bioreceptor to detect UspA2. After cell-systematic evolution of ligands by exponential enrichment (cell-SELEX) was performed, next-generation sequencing was used to sequence the final aptamer pool. The most frequent aptamer sequences were further evaluated using bioinformatic tools. The two most promising aptamer candidates, Apt1 and Apt1_RC (Apt1 reverse complement), had Kd values of 214.4 and 3.4 nM, respectively. Finally, a simple and label-free electrochemical biosensor was functionalized with Apt1_RC. The aptasensor surface modifications were confirmed by impedance spectroscopy and cyclic voltammetry. The ability to detect UspA2 was evaluated by square wave voltammetry, exhibiting a linear detection range of 4.0 × 104–7.0 × 107 CFU mL−1, a square correlation coefficient superior to 0.99 and a limit of detection of 4.0 × 104 CFU mL−1 at pH 5.0. The workflow described has the potential to be part of a sensitive PoC diagnostic platform to detect and quantify M. catarrhalis from biological samples.

show abstract

On-site enrichment and detection of live Salmonella typhimurium using a bioluminescence sensor coupled with a hyperbranched aptamer probe-labelled stir-bars array

Cited by 14 publications

References 28 publications

Application of ATP-based bioluminescence technology in bacterial detection: a review

Application of ATP-based bioluminescence technology in bacterial detection: a review

Emerging Biomimetic Sensor Technologies for the Detection of Pathogenic Bacteria: A Commercial Viability Study

Aptasensor for the Detection of Moraxella catarrhalis Adhesin UspA2

Contact Info

Product

Resources

About