On-Site Toluidine Blue Staining and Screening Improves Efficiency of Fine-Needle Aspiration Cytology Reporting

Ammanagi, A S; Dombale, V D; Patil, Sharada S

doi:10.1159/000338725

Cited by 18 publications

(32 citation statements)

References 13 publications

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“…Ammanagi et al [1] recently investigated the role of toluidine blue for rapid staining in assessment of aspiration adequacy and reported an improvement in the efficiency of FNA. Borget et al [4] concluded that on-site staining should dramatically reduce the rate of failures and thus enable same-day reporting and repeat sampling.…”

Section: Discussionmentioning

confidence: 99%

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On-Spot Brilliant Cresyl Blue Staining in Fine Needle Aspiration, a Novel Technique for Cytological Diagnosis

Sofi¹,

Sherwani²,

Hasan³

et al. 2013

Acta Cytologica

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Objective: Application of on-spot staining with brilliant cresyl blue, a supravital stain, was investigated in fine needle aspirates from breast, lymph node and thyroid. Study Design: Patients with palpable breast lumps, lymph nodes and thyroid nodules were included in the study. Brilliant cresyl blue was used to stain the smears, which were evaluated for smear adequacy as well as cytological diagnosis. Smears were decolorized and subjected to Pap and/or HE staining for comparison. Results: Smears which were deemed hemorrhagic on the first attempt with brilliant cresyl blue correlated with Pap staining; then sites were reaspirated within minutes to obtain satisfactory cellular smears. Morphologically, cells which were designated malignant on supravital staining were mostly malignant on Pap/HE staining. Conclusion: On-spot brilliant cresyl blue staining increases the diagnostic potential on preliminary examination by decreasing the staining time and is as good as conventional stains in exhibiting cellular morphology.

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Section: Discussionmentioning

confidence: 99%

“…Rapid staining and urgent reporting are cornerstones of clinical setups, particularly in the surgical setting, which can be equivalent to frozen section examination [1]. On spot (on-site) interpretation for sample adequacy in FNA smears improves diagnostic yield even in ultrasound-guided FNA [2,3].…”

Section: Introductionmentioning

confidence: 99%

On-Spot Brilliant Cresyl Blue Staining in Fine Needle Aspiration, a Novel Technique for Cytological Diagnosis

Sofi¹,

Sherwani²,

Hasan³

et al. 2013

Acta Cytologica

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“…In the study of Ammanagi et al, there was 14% of unsatisfactory smears (28/200 cases) at the first biopsy attempt, which was assessed by ROSE, mainly in thyroid, followed by breast and lymph nodes. After repeat FNAC in the same visit, adequate material was found in 24/28 cases.…”

Section: Resultsmentioning

confidence: 98%

“…The advent of “one‐stop” diagnostic services (with rapid on site evaluation (ROSE) and image‐guided procedures, as well as liquid‐based cytology will also improve the diagnostic yield. In a one‐stop FNAC clinic, the cellularity of smears can be assessed at the time of the biopsy giving immediate feedback about the quality of the biopsy technique and the smears.…”

Section: Resultsmentioning

confidence: 99%

“…Pathologists can have an important role, not only in reading the smears, but also in performing the FNAC technique, since they can see the patient and perform a physical examination, which is important to improve the integration of clinical and pathological findings. In addition, it is possible to perform quick staining and rapid on site evaluation of the sample to assess its adequacy in real time and repeat the procedure immediately if necessary …”

Section: Introductionmentioning

confidence: 99%

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Fine needle aspiration cytology in Mozambique: Report of a 15‐year experience

Carrilho

Ismail

Lorenzoni

et al. 2018

Diagnostic Cytopathology

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Background Fine needle aspiration cytology (FNAC) is an important diagnostic tool in a range of medical settings. It is fast, quick and a highly accurate diagnostic method and can be used, in settings with minimal laboratory infrastructures. Methods In this report, we describe the experience in the use of FNAC since it is introduction in 1996 in the Anatomical Pathology Service of the Maputo Central Hospital (MCH), along with more detailed data referring to 2009‐2010. Results The number of FNAC analyses increased gradually from 269 (4.1% of all pathologic tests of the Service) in 1996, when it was introduced in Mozambique, to 3234 (17% of all tests) in 2010. Lymph nodes were the organs most frequently biopsied, followed by breast and soft tissues. Inflammatory conditions, especially tuberculosis, were the most frequent diagnoses (22.2% of the cases), followed by hyperplastic conditions (20.6%), benign tumors (13.4%) and malignant tumors (12.3%). Conclusion Our results clearly demonstrate that even in an environment with poor laboratory resources, it is possible to establish a FNAC clinic that can provide a quick and precise diagnosis for clinicians to aid in early treatment interventions, especially in inflammatory diseases which were the majority of our cases.

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Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness

Agarwal

Toi

Subramaniam

et al. 2018

Diagnostic Cytopathology

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Objective Rapid on‐site evaluation (ROSE) is a technique beneficial in determining the adequacy of the samples, thereby increasing the diagnostic yield, useful in triage of specimens for ancillary studies and can also help determine a preliminary diagnosis in emergency cases. The different rapid stains for on‐site evaluation described in the literature are diff quik, toluidine blue (TB), brilliant cresyl blue (BCB), ultra‐fast Pap stains, and rapid hematoxylin and eosin (H&E). This study was undertaken as there is sparse literature regarding the best and the most cost‐effective rapid stain. Method Fine needle aspiration samples from 200 patients with palpable swellings in easily accessible regions were taken. Smears stained by rapid and routine stains were assessed based on four parameters, with provisional diagnosis on the rapid stained smears. A comparative analysis of the advantages and disadvantages of the rapid stains was carried out with appropriate statistical tests with the routinely stained smears as gold standard. Results There was adequate material in 100% of ROSE smears. rapid pap stained smears showed well preserved cytoplasmic details, nuclear details, and background details. The time taken was least with TB and BCB being 5 s each. The most cost‐effective was found to be TB. Conclusions We conclude that TB is the most cost‐effective, quick, least labor‐intensive, and reliable rapid stain for ROSE especially in resource‐poor settings.

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On-Site Toluidine Blue Staining and Screening Improves Efficiency of Fine-Needle Aspiration Cytology Reporting

Cited by 18 publications

References 13 publications

On-Spot Brilliant Cresyl Blue Staining in Fine Needle Aspiration, a Novel Technique for Cytological Diagnosis

On-Spot Brilliant Cresyl Blue Staining in Fine Needle Aspiration, a Novel Technique for Cytological Diagnosis

Fine needle aspiration cytology in Mozambique: Report of a 15‐year experience

Prospective comparison of cytological specimen adequacy assessment by different rapid staining techniques for rapid on‐site evaluation in fine needle aspiration cytology and their cost‐effectiveness

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