On Some Properties of the Jelly Coat in Oocytes and Mature Eggs of Sea Urchins. A Study of Phase-Dependent Changes of Metaplasmic Layers in the Cell Surface

Runnström, John

doi:10.2307/1539350

Cited by 9 publications

(1 citation statement)

References 40 publications

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“…The presence of jelly coat splitting enzymes has been established in sperm (Hartmann & Sohartau, 1939;Hultin & Lundblad, 1952;Runnstrom, 1964;Vasseur, 1948;, but the significance of the depolymerization of the jelly coat has been obscured by the fact that it has been looked upon primarily as the means for the sperm to reach the egg surface and the bearer of sperm agglutinating factors. Another possibility exists, namely, that the depolymerization of the jelly coat is actually a part of the activation process releasing a high local concentration of monovalent cations and thereby effecting an ion exchange response in the closely adhering surface mps.…”

Section: Fertilizationmentioning

confidence: 99%

A Proposed Role for Mucopolysaccharides in the Initiation and Control of Cell Division*,†

Lippman

1965

Trans NY Acad Sci

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Section: Fertilizationmentioning

confidence: 99%

A Proposed Role for Mucopolysaccharides in the Initiation and Control of Cell Division*,†

Lippman

1965

Trans NY Acad Sci

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A species‐specific sperm factor dispersing the jelly coat of the egg of the sea urchin, Anthocidaris crassispina

Yamada

Aketa

1983

Gamete Research

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A species-specific factor capable of dispersing the jelly coat surrounding eggs has been purified from sperm of the sea urchin, Anthocidaris crassispina. It does not exert its effect on the vitelline layer. The purification has been accomplished by a four-step procedure involving ammonium sulfate fractionation, gel filtration on Sepharose CL-4B, ion-exchange column chromatography on DEAE-cellulose, and affinity column chromatography on heparin-Sepharose CL-6B. The isolated factor is homogenous in sodium dodecyl sulfate polyacrylamide gel electrophoresis in the presence or absence of 0-rnercaptoethanol, estimated molecular weight being about 140,000.The jelly dispersion by the present factor is activated by CaCI2, and inhibited by KCI, MnCI2, EDTA, and EGTA, and by sulfated saccharides such as chondroitin sulfate A and C, heparin, and glucose-6-sulfate. Inorganic sulfates such as (NH&S04 and Na2S04 have no effect on jelly dispersion. This factor is heat-labile, losing its activity in 30 min at 50°C.The present factor is found also in the seminal plasma, and released from sperm themselves by treatment with Triton X-100. These results suggest that this factor is loosely bound to the sperm surface. Although glycosidase and arylsulfatase activities are detectable in the seminal plasma, these enzyme activities are not detectable in the purified jelly dispersing factor.Only trypsin and or-chymotrypsin among commercial enzymes tested disperse the jelly coat, but neither activity is detectable in our preparation. The jelly-dispersing activity is inhibited neither by trypsin inhibitors such as N-a-p-tosyl-L-lysine-chloromethyl ketone, soybean trypsin inhibitor, ovomucoid trypsin inhibitor, nor by chymotrypsin inhibitors such as L-I-tosylamide-2-phenyl-ethylchloromethyl ketone and chymostatin. Participation of trypsin-like and chymotrypsin-like enzymes in jelly dispersion seems unlikely.

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Gametogenesis

Piatigorsky¹

1975

The Sea Urchin Embryo

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On Some Properties of the Jelly Coat in Oocytes and Mature Eggs of Sea Urchins. A Study of Phase-Dependent Changes of Metaplasmic Layers in the Cell Surface

Cited by 9 publications

References 40 publications

A Proposed Role for Mucopolysaccharides in the Initiation and Control of Cell Division*,†

A Proposed Role for Mucopolysaccharides in the Initiation and Control of Cell Division*,†

A species‐specific sperm factor dispersing the jelly coat of the egg of the sea urchin, Anthocidaris crassispina

Gametogenesis

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