An annotated checklist of spiders of the Khibiny Mountains (Kola Peninsula, Russia) based on both original and literature-derived data is presented for the first time. The list contains 161 species belonging to 17 families. Twenty species (Araniella proxima, Cyclosa conica, Gnaphosa bicolor, G. lapponum, G. leporina, Zelotes clivicola, Agyneta affinis, A. rurestris, Ceratinella wideri, Dicymbium nigrum, Drapetisca socialis, Entelecara erythropus, Moebelia penicillata, Oedothorax apicatus, Porrhomma campbelli, Agroeca lusatica, Alopecosa pinetorum, A. taeniata, Philodromus cespitum, Titanoeca nivalis) are recorded from Kola Peninsula for the first time. A preliminary analysis of the studied fauna is presented.