On the 50th Anniversary of the discovery of autoinduction and the ensuing birth of quorum sensing

Nealson, Kenneth H.

doi:10.1111/1462-2920.14928

Cited by 2 publications

(4 citation statements)

References 19 publications

(35 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PptAB might also be able to export the complementary sequence of the mature form of SHP* 1358 (i.e., the N-terminal peptide that results from the maturation of SHP* 1358 [SHP* 1358 (1-15) ]) and the related products of degradation. Therefore, using the TIL1213 strain (∆amiCDE pBV5030::P 32 -shp 1358 ), we looked for traces of SHP* 1358 (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) as well as for fragments that result from shortening at the N-terminus (from SHP* 1358 (1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15) to SHP* 1358 (7)(8)(9)(10)(11)(12)(13)(14)(15) ) and at the C-terminus (from SHP* 1358 (1)(2)(3)(4)(5)(6)(7)(8)…”

Section: Pptab Only Exports the Mature Form Of Shp 1358mentioning

confidence: 99%

“…Quorum sensing (QS) is a cell-cell communication mechanism that is used by bacteria to trigger the expression of an entire set of genes when population levels exceed a certain threshold of cell density [ 1 ]. Functions that are controlled by QS are very diverse, ranging from luminescence in Vibrio fischeri [ 2 ], natural transformation in streptococci [ 3 ], bacteriocin production in lactococci and bacilli [ 4 ], and virulence in Staphylococcus aureus [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

“…Most, however, remain unknown. Peptides associated with PrgX-like regulators fall into one of two categories: (1) they are inhibitory peptides encoded by short genes or (2) they are activating peptides that are embedded in lipoprotein secretion signal peptides that are released outside the cell during lipoprotein export. Activating peptides are matured by serial peptidases, which include a lipoprotein signal peptidase, a transmembrane protease, named Eep, and additional undescribed exoproteases.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Export of Rgg Quorum Sensing Peptides is Mediated by the PptAB ABC Transporter in Streptococcus Thermophilus Strain LMD-9

Lingeswaran

Metton

Henry

et al. 2020

Genes

View full text Add to dashboard Cite

In streptococci, intracellular quorum sensing pathways are based on quorum-sensing systems that are responsible for peptide secretion, maturation, and reimport. These peptides then interact with Rgg or ComR transcriptional regulators in the Rap, Rgg, NprR, PlcR, and PrgX (RRNPP) family, whose members are found in Gram-positive bacteria. Short hydrophobic peptides (SHP) interact with Rgg whereas ComS peptides interact with ComR regulators. To date, in Streptococcus thermophilus, peptide secretion, maturation, and extracellular fate have received little attention, even though this species has several (at least five) genes encoding Rgg regulators and one encoding a ComR regulator. We studied pheromone export in this species, focusing our attention on PptAB, which is an exporter of signaling peptides previously identified in Enterococcus faecalis, pathogenic streptococci and Staphylococcus aureus. In the S. thermophilus strain LMD-9, we showed that PptAB controlled three regulation systems, two SHP/Rgg systems (SHP/Rgg1358 and SHP/Rgg1299), and the ComS/ComR system, while using transcriptional fusions and that PptAB helped to produce and export at least three different mature SHPs (SHP1358, SHP1299, and SHP279) peptides while using liquid chromatography-tandem mass spectrometry (LC-MS/MS). Using a deep sequencing approach (RNAseq), we showed that the exporter PptAB, the membrane protease Eep, and the oligopeptide importer Ami controlled the transcription of the genes that were located downstream from the five non-truncated rgg genes as well as few distal genes. This led us to propose that the five non-truncated shp/rgg loci were functional. Only three shp genes were expressed in our experimental condition. Thus, this transcriptome analysis also highlighted the complex interconnected network that exists between SHP/Rgg systems, where a few homologous signaling peptides likely interact with different regulators.

show abstract

Section: Pptab Only Exports the Mature Form Of Shp 1358mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Export of Rgg Quorum Sensing Peptides is Mediated by the PptAB ABC Transporter in Streptococcus Thermophilus Strain LMD-9

Lingeswaran

Metton

Henry

et al. 2020

Genes

View full text Add to dashboard Cite

show abstract

“…In this communication we investigate an alternative to the simplistic promoter‐reporter fusion design: the introduction of quorum sensing (QS) elements as means of signal amplification. This natural microbial mechanism, originally described in bioluminescent bacteria as “autoinduction” [ 15 ], is involved in diverse processes such as symbiosis, virulence, competence, conjugation, antibiotic production, motility, sporulation, and biofilm formation. The accumulation of a small membrane‐permeable molecule promotes the activation of specific genes in response to changes in the density of the microbial community.…”

Section: Introductionmentioning

confidence: 99%

Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives’ detection capabilities

Shpigel

Nathansohn

Glozman

et al. 2022

Engineering in Life Sciences

View full text Add to dashboard Cite

A possible solution for the standoff detection of buried landmines is based on the use of microbial bioreporters, genetically engineered to emit a remotely detectable optical signal in response to trace amounts of explosives’ signature chemicals, mostly 2,4‐dinitrotoluene (DNT). Previously developed DNT sensor strains were based on the fusion of a DNT‐inducible gene promoter to a reporting element, either a fluorescent protein gene or a bacterial bioluminescence gene cassette. In the present study, a different approach was used: the DNT‐inducible promoter activates, in Escherichia coli, the quorum‐sensing luxI and luxR genes of Aliivibrio fischeri. N‐Acyl homoserine lactone (AHL), synthesized by LuxI, combines with LuxR and activates the bioluminescence reporter genes. The resulting bioreporter displayed a dose‐dependent luminescent signal in the presence of DNT. Performance of the sensor strain was further enhanced by manipulation of the sensing element (combining the E. coli DNT‐inducible azoR and yqjF gene promoters), by replacing the luminescence gene cassette of Photorhabdus luminescens luxCDABE with A. fischeri luxCDABEG, and by introducing two mutations, eutE and ygdD, into the host strain. DNT detection sensitivity of the final bioreporter was over 340‐fold higher than the original construct.

show abstract

On the 50th Anniversary of the discovery of autoinduction and the ensuing birth of quorum sensing

Cited by 2 publications

References 19 publications

Export of Rgg Quorum Sensing Peptides is Mediated by the PptAB ABC Transporter in Streptococcus Thermophilus Strain LMD-9

Export of Rgg Quorum Sensing Peptides is Mediated by the PptAB ABC Transporter in Streptococcus Thermophilus Strain LMD-9

Introduction of quorum sensing elements into bacterial bioreporter circuits enhances explosives’ detection capabilities

Contact Info

Product

Resources

About