On the action of ozone on gelatin

Cataldo, Franco

doi:10.1016/j.ijbiomac.2007.02.008

Cited by 15 publications

(10 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Besides, the long reaction time is necessary for the formation of hexagonal structure of FA nanocrystals. Table 3 The frequencies of components in infrared spectra of reference gelatin [41][42][43][44].…”

Section: Discussionmentioning

confidence: 99%

Size-controlled synthesis and characterization of fluorapatite nanocrystals in the presence of gelatin

Wang

Sun

et al. 2011

Powder Technology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Size-controlled synthesis and characterization of fluorapatite nanocrystals in the presence of gelatin

Wang

Sun

et al. 2011

Powder Technology

View full text Add to dashboard Cite

“…The information in the literature about the effect of ozone on hydrogels is very scarce. However good results were obtained with gelatin of animal origin [41]. …”

Section: Introductionmentioning

confidence: 99%

About the Sterilization of Chitosan Hydrogel Nanoparticles

et al. 2016

View full text Add to dashboard Cite

In the last years, nanostructured biomaterials have raised a great interest as platforms for delivery of drugs, genes, imaging agents and for tissue engineering applications. In particular, hydrogel nanoparticles (HNP) associate the distinctive features of hydrogels (high water uptake capacity, biocompatibility) with the advantages of being possible to tailor its physicochemical properties at nano-scale to increase solubility, immunocompatibility and cellular uptake. In order to be safe, HNP for biomedical applications, such as injectable or ophthalmic formulations, must be sterile. Literature is very scarce with respect to sterilization effects on nanostructured systems, and even more in what concerns HNP. This work aims to evaluate the effect and effectiveness of different sterilization methods on chitosan (CS) hydrogel nanoparticles. In addition to conventional methods (steam autoclave and gamma irradiation), a recent ozone-based method of sterilization was also tested. A model chitosan-tripolyphosphate (TPP) hydrogel nanoparticles (CS-HNP), with a broad spectrum of possible applications was produced and sterilized in the absence and in the presence of protective sugars (glucose and mannitol). Properties like size, zeta potential, absorbance, morphology, chemical structure and cytotoxicity were evaluated. It was found that the CS-HNP degrade by autoclaving and that sugars have no protective effect. Concerning gamma irradiation, the formation of agglomerates was observed, compromising the suspension stability. However, the nanoparticles resistance increases considerably in the presence of the sugars. Ozone sterilization did not lead to significant physical adverse effects, however, slight toxicity signs were observed, contrarily to gamma irradiation where no detectable changes on cells were found. Ozonation in the presence of sugars avoided cytotoxicity. Nevertheless, some chemical alterations were observed in the nanoparticles.

show abstract

“…The interest of the action of ozone on biological macromolecules (Cataldo 2003(Cataldo , 2004(Cataldo , 2005Cataldo 2006aCataldo , 2006bCataldo , 2007Cataldo and Gentilini 2005; Von Sonntag and Von Gunten 2012) was re-awakened because of the current coronavirus pandemic situation. While we know quite well as ozone reacts with proteins (Cataldo 2003(Cataldo , 2004(Cataldo , 2007Cataldo and Gentilini 2005) and with RNA and DNA (Cataldo 2005(Cataldo , 2006aTheruvathu et al 2001;Von Sonntag and Von Gunten 2012;Young et al 2020), our knowledge is somewhat more limited regarding the reaction of ozone with PPL, the key constituent of cell membranes and pericapsidic viruses like coronavirus. Some reference work on PPL ozonation were performed by Pryor, Squadrito, and Friedman (1995) but framed in another context of ozone toxicity on lungs and lung cells.…”

Section: Introductionmentioning

confidence: 99%

On the Action of Ozone on Phospholipids, a Model Compound of the External Envelope of Pericapsidic Viruses like Coronavirus. Part 1

Cataldo¹,

Ori²

2020

Ozone: Science & Engineering

Self Cite

View full text Add to dashboard Cite

Coronavirus is a virus with an external protective membrane known as pericapsid and composed essentially by a double layer of phospholipids (PPL) and other components. The destabilization or the decomposition of this pericapsid means the certain inactivation of the coronavirus. The purpose of this work is to show that ozone is able to oxidize easily the phospholipids destabilizing the micellar state of phospholipidic model compounds derived from soy lecithin. PPL were ozonized in reversal micelle state in n-hexane and in normal micelle state in aqueous emulsion. The progress of PPL ozonation was followed spectrophotometrically and iodometrically. It was found that in n-hexane PPL ozonides (PPL-O) start to precipitate at O 3 /PPL = 1.5 molar ratio and the precipitation is complete at a molar ratio = 3, confirming the average presence of three double bonds per PPL molecule. The pseudofirst order kinetics of the reverse micelle destabilization oxidation to PPL-O was determined in the range of 1.98-6.75 × 10 −3 s −1. On the other hand, PPL in aqueous medium forms a milky emulsion with the PPL molecules arranged in the micelle. The action of ozone on this emulsion causes the complete destabilization of the micelles due to the oxidation of the unsaturated fatty chains of PPL and at O 3 /PPL = 3 molar ratio the solution appears completely transparent, i.e. the original micellar state is disappeared at a rate of 3.0 × 10 −3 s −1. PPL-O was also studied by FT-IR, DSC and polarimetry. The results derived from the ozonation of PPL model compound may be applied to the PPL double layer of coronavirus pericapsid, confirming the effectiveness of ozone in coronavirus inactivation.

show abstract

On the action of ozone on gelatin

Cited by 15 publications

References 11 publications

Size-controlled synthesis and characterization of fluorapatite nanocrystals in the presence of gelatin

Size-controlled synthesis and characterization of fluorapatite nanocrystals in the presence of gelatin

About the Sterilization of Chitosan Hydrogel Nanoparticles

On the Action of Ozone on Phospholipids, a Model Compound of the External Envelope of Pericapsidic Viruses like Coronavirus. Part 1

Contact Info

Product

Resources

About