On the Adsorption of Proteins on Solid Surfaces, a Common but Very Complicated Phenomenon.

Abstract

Adsorption of proteins on solid surfaces and their interaction are major concerns in a number of fields such as biology, medicine, biotechnology and food processing, and play an important role from various points of view. Based on practical viewpoints, information on the conformation of the adsorbed protein as well as adsorption characteristics is essential for a system's performance. Although there are still many problems to be solved, extensive studies in recent years, owing to the development in instrumentation and instrumental techniques, reveal the adsorption behavior of proteins in detail. Here, we stress the importance and interesting aspect of protein adsorption on solid surfaces by reviewing findings that have been obtained in recent years.

Calcium phosphate-alginate microspheres as enzyme delivery matrices

“…Some aspects remain, however, to be investigated, namely the activity of the enzyme when released from the matrices used in this work (ongoing studies). Although protein immobilisation, either by entrapment or adsorption, often results in conformational alterations that may render the protein inactive, several authors have reported improved enzyme activity and/or stability in immobilised preparations when compared to their free forms [40].…”

Silica Surface Features and Their Role in the Adsorption of Biomolecules: Computational Modeling and Experiments

“…Therefore it is of crucial importance for many biological and biomedical applications to know if the protein structure will be conserved upon adsorption. 260,261 The conventional answer is negative: adsorption, in particular on silica, changes the folding pattern of the protein chain, in other words it causes denaturation.…”

Adsorption of human serum albumin on the chrysotile surface: a molecular dynamics and spectroscopic investigation

“…In addition, HSA-coated chrysotile nanocrystals have been investigated by FTIR spectroscopy as a function of the protein concentration which induces a different degree of surface coverage. Surface coating extent (G) represents the amount of protein adsorbed (mg m K2 ) at constant temperature on a given substrate and strongly depends on the surface features: a hydrophilic substrate, like chrysotile, may adsorb a much greater protein amount than a hydrophobic one (Nakanishi et al 2001). It is important to take into account that at pH 7.4, HSA (isoelectric point 4.6) and chrysotile (isoelectric point 8-12, according to the different amount of magnesia) are predominantly negatively and positively charged, respectively; thus electrostatic forces play here an important role.…”