Numerical simulations as well as recent experiments in turbulent three-dimensional flow show a trend of the gradient of a passive scalar to align with the compressional axis of the strain tensor. In two-dimensional flow, however, it has been proved that the most probable orientation of the scalar gradient can be different from the compressional direction. An idealized situation is used to address this question in the three-dimensional case and to suggest a possible way to reexamine the scalar gradient alignment in three-dimensional flow. This kind of analysis can be applied to the material line alignment as well.Enhancement of mixing in turbulent flow is closely linked to gradient amplification through small scale production. The mean dissipation rate ͗⑀ ͘ of the energy of fluctuations of a scalar is proportional to the variance of the fluctuating gradient through ͗⑀ ͘ = D͗g ␣ g ␣ ͘, where D is the molecular diffusivity of and g i = ץ / ץx i . The gradient norm and thus dissipation are constantly promoted by strain s. This process is expressed by the positivity of the mean production term −͗g ␣ s ␣ g  ͘ and is, in fact, a kinematic property of the scalar field. 1 Writing the instantaneous production term in the strain basis reveals that the positivity of its mean value is promoted by alignment of the scalar gradient with the compressional direction 1 − g ␣ s ␣ g  = − ͉g͉ 2 ͓ 1 cos 2 ͑e 1 ,g͒ + 2 cos 2 ͑e 2 ,g͒ + 3 cos 2 ͑e 3 ,g͔͒, ͑1͒where the strain eigenvalues are such that 1 ജ 2 ജ 3 , 1 ജ 0, 3 ഛ 0, and, if incompressibility is assumed, 1 + 2 + 3 = 0. The strain eigenvectors e 1 , e 2 , and e 3 define, respectively, the dilatation, "intermediate," and compression axes of strain. From Eq. ͑1͒ and the signs of the i 's, it is clear that the better the alignment of g with e 3 , the more positive the production term. Alignment properties of the scalar gradient, then, are essential to the mixing process. Now, in three-dimensional turbulence, numerical simulations 1-5 as well as recent experimental data 6 appear to show a trend of the scalar gradient to statistically align with the compressional direction. More precisely, the data only prove that the scalar gradient better aligns with the compression axis than with the other strain directions. This also is a kinematic property. 1 Note that mean shear, however, seems to weaken the alignment of the gradient with the compressional direction. 2,3 Even so, the statistical alignment property of the scalar gradient is actually not easy to explain, for the compressional direction is in general not a fixed point of the gradient orientation equations. The compressional direction is the stable fixed point in the special case of a pure, stationary strain, but when vorticity and/or rotation of the strain basis also are present, the existence of an equilibrium orientation for the gradient is generally not proved. 7 Anyway, the equilibrium orientation, if any, is certainly determined by the combined actions of strain, effective rotation ͑i.e., vorticity plus strain basis ro...