On the application of ‘seeding’ techniques in the primary separation of plasmid DNA from neutralised <i>E. coli</i> lysates

Theodosiou, Eirini; Thomas, Owen R.T.

doi:10.1002/jctb.1834

Cited by 3 publications

(2 citation statements)

References 37 publications

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“…After cell lysis, the CMNPs can be further utilized to remove cellular impurities [19,34] or directly capture target biomolecules [14,[23][24][25]. In the present study, the PCR template preparation procedure was simplified by subjecting the nanoparticle/cell complexes directly to lysis without prior removal of the magnetic nanoparticles.…”

Section: Resultsmentioning

confidence: 99%

PCR-ready human DNA extraction from urine samples using magnetic nanoparticles

Shan

Zhou

Chen

et al. 2012

Journal of Chromatography B

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Section: Resultsmentioning

confidence: 99%

PCR-ready human DNA extraction from urine samples using magnetic nanoparticles

Shan

Zhou

Chen

et al. 2012

Journal of Chromatography B

View full text Add to dashboard Cite

“…Their properties dictate that their efficient large-scale manufacture must follow a very different 'general' path to that established for therapeutic human proteins of much smaller dimensions [13,[16][17][18][19][20][21]. Current protocols for the purification of plasmid DNA show 15 heavy reliance on packed bed chromatography -centred on capture by anion exchange (AEC) adsorption, followed by polishing of the salt-eluted fraction by size exclusion chromatography [16,17,19,21].…”

mentioning

confidence: 99%

Surface modification of chromatography adsorbents by low temperature low pressure plasma

Arpanaei¹,

Winther‐Jensen²,

Theodosiou³

et al. 2010

Journal of Chromatography A

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Bioseparation: The limiting step in bioprocess development

Rito‐Palomares

2008

J of Chemical Tech & Biotech

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Editorial Bioseparation: The limiting step in bioprocess developmentBioseparation can be defined as the set of sequential unit operations which, in a bioprocess, result in the recovery and purification of biological products -including proteins, enzymes, vaccines, colorants or antibodies. These are usually commercialized according to their biological function, and it is easy to anticipate that the bioseparation processes required to obtain, for example, industrial enzymes and oral or injected delivery products will differ greatly. It has been generally recognized that the recovery and purification stages (i.e. bioseparation), because the purity needed varies, represent the limiting steps in process development -but despite recent investigations, in particular from the industry, extensive research in this area has generally been neglected. Although several explanations for this trend can be given, we would like to highlight three major factors: (i) historical effect, (ii) process complexity and (iii) regulatory and validation issues. The historical effect is attributed to the need to develop the production stage of bioprocesses first. Thus, the bioprocesses initially developed to bring biological products to the market were strongly oriented towards the optimization of the fermentation step -neglecting the bioseparation part. This tendency was further accentuated by process complexity issues as fermentation, representing only one unit operation in comparison with typically more than ten unit operations for the bioseparation part of the process, was more thoroughly investigated. Finally, it is well-known that the implementation of advances in bioseparation processes has been hindered by validation and cost constraints. Regulatory and validation considerations have promoted a reluctance to embrace an entirely new technology, in this and other fields of biochemical engineering and biotechnology.In this Special Issue, JCTB presents a particular approach to trends and challenges in bioseparation. We have collected a Mini-Review, a Perspective, three Reviews and seven original research papers to outline the evolution of the type of problems, as well as the technologies, encountered in the field of product recovery and purification.The past, present and future challenges in efficient separations of recombinant proteins are nicely addressed by Juan Asenjo and Barbara Andrews in the first Mini-Review 1 . It raises how the challenges faced by bioseparation have changed over the past few years. The three main issues addressed are the increase in product concentration, the change of paradigm regarding the use of protein A approach and the need for complex and sophisticated mathematical models -key issue that will require particular attention from both academics and industrialists. The future application and industrial implementation of in-situ product removal (ISPR) techniques are addressed in the Perspective by John Woodley and co-workers 2 . The findings reported there are the results of a round-table discussion held at the...

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On the application of ‘seeding’ techniques in the primary separation of plasmid DNA from neutralised E. coli lysates

Abstract: • This is a preprint of an article published in the Jour-

Cited by 3 publications

References 37 publications

PCR-ready human DNA extraction from urine samples using magnetic nanoparticles

PCR-ready human DNA extraction from urine samples using magnetic nanoparticles

Surface modification of chromatography adsorbents by low temperature low pressure plasma

Bioseparation: The limiting step in bioprocess development

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