On the binding of complement to solid artificial surfaces in vitro

Wetterö, Jonas; Askendal, Agneta; Bengtsson, Torbjörn; Tengvall, Pentti

doi:10.1016/s0142-9612(01)00203-4

Cited by 54 publications

(36 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These changes have previously been shown to increase with an increase in hydrophobicity of the surface for a number of proteins including IgG 32,33 (see also the Supplementary data for detailed information on QCM-D measurements of IgG adsorption). An increase in IC activation by precoated IgG on hydrophobic surfaces has earlier been reported by Wettero et al 34 and has been proposed to originate from surface-induced conformational changes of the IgG molecule. 35 However, in the attempt to augment the activation of the IC by pre-adsorption of IgG, high activation was found on smooth surfaces regardless of their hydrophobicity (compare left groups in Figures 7 and 8).…”

supporting

confidence: 61%

Immune complement activation is attenuated by surface nanotopography

Hulander¹,

Lundgren²,

Berglin³

et al. 2011

IJN

View full text Add to dashboard Cite

The immune complement (IC) is a cell-free protein cascade system, and the first part of the innate immune system to recognize foreign objects that enter the body. Elevated activation of the system from, for example, biomaterials or medical devices can result in both local and systemic adverse effects and eventually loss of function or rejection of the biomaterial. Here, the researchers have studied the effect of surface nanotopography on the activation of the IC system. By a simple nonlithographic process, gold nanoparticles with an average size of 58 nm were immobilized on a smooth gold substrate, creating surfaces where a nanostructure is introduced without changing the surface chemistry. The activation of the IC on smooth and nanostructured surfaces was viewed with fluorescence microscopy and quantified with quartz crystal microbalance with dissipation monitoring in human serum. Additionally, the ability of pre-adsorbed human immunoglobulin G (IgG) (a potent activator of the IC) to activate the IC after a change in surface hydrophobicity was studied. It was found that the activation of the IC was significantly attenuated on nanostructured surfaces with nearly a 50% reduction, even after pre-adsorption with IgG. An increase in surface hydrophobicity blunted this effect. The possible role of the curvature of the nanoparticles for the orientation of adsorbed IgG molecules, and how this can affect the subsequent activation of the IC, are discussed. The present findings are important for further understanding of how surface nanotopography affects complex protein adsorption, and for the future development of biomaterials and blood-contacting devices.

show abstract

supporting

confidence: 61%

Immune complement activation is attenuated by surface nanotopography

Hulander¹,

Lundgren²,

Berglin³

et al. 2011

IJN

View full text Add to dashboard Cite

show abstract

“…18,19 To examine protein adsorption with null-ellipsometry in parallel to adhesion experiments performed on glass slides, silicon wafers (Wacker-Chemie GmbH, Munich, Germany) were used, as this method requires a reflective surface. This approach has previously been evaluated and successfully used in order to create model surfaces, which are suitable for investigating protein cell interaction.…”

Section: B Surface Methylationmentioning

confidence: 99%

Toll like receptor 2/1 mediated platelet adhesion and activation on bacterial mimetic surfaces is dependent on src/Syk-signaling and purinergic receptor P2X1 and P2Y12 activation

et al. 2014

Self Cite

View full text Add to dashboard Cite

Platelets are considered to have important functions in inflammatory processes as key players in innate immunity. Toll like receptors (TLRs), expressed on platelets, recognize pathogen associated molecular patterns and trigger immune responses. Pathogens are able to adhere to human tissues and form biofilms which cause a continuous activation of the immune system. The authors aimed to investigate how immobilized Pam3CSK4 (a synthetic TLR2/1 agonist) and IgG, respectively, resembling a bacterial focus, affects adhesion and activation of platelets including release of two cytokines, regulated on activation normal T-cell expressed and secreted (RANTES) and macrophage migration inhibitory factor (MIF). The authors also aim to clarify the signaling downstream of TLR2/1 and FcγRII (IgG receptor) and the role of adenine nucleotides in this process. Biolayers of Pam3CSK4 and IgG, respectively, were confirmed by null-ellipsometry and contact angle measurements. Platelets were preincubated with signaling inhibitors for scr and Syk and antagonists for P2X1 or P2Y1 [adenosine triphosphate (ATP), adenosine diphosphate (ADP) receptors] prior to addition to the surfaces. The authors show that platelets adhere and spread on both Pam3CSK4- and IgG-coated surfaces and that this process is antagonized by scr and Syc inhibitors as well as P2X1 and P2Y antagonists. This suggests that Pam3CSK4 activated platelets utilize the same pathway as FcγRII. Moreover, the authors show that ATP-ligation of P2X1 is of importance for further platelet activation after TLR2/1-activation, and that P2Y12 is the prominent ADP-receptor involved in adhesion and spreading. RANTES and MIF were secreted over time from platelets adhering to the coated surfaces, but no MIF was released upon stimulation with soluble Pam3CSK4. These results clarify the importance of TLR2/1 and FcγRII in platelet adhesion and activation, and strengthen the role of platelets as an active player in sensing bacterial infections.

show abstract

“…Since the realization of a complement activation capacity by artificial surfaces upon contact with blood, a common belief has evolved that nucleophilic surface groups such as hydroxyl and amine react with and eventually bind to the internal thioester in C3. 31 The drawback of the regenerated cellulose is the presence of hydroxyl groups that trigger the complement activation and transient leukopenia. As a polyhydroxyl compound, PVA membrane may provide possible analogies with those of cellulose.…”

Section: Resultsmentioning

confidence: 99%

Plasma protein adsorption to anion substituted poly(vinyl alcohol) membranes

et al. 2003

View full text Add to dashboard Cite

Anion-substituted poly(vinyl alcohol) (PVA) membranes, carboxymethylated PVA (C-PVA), and sulfonated PVA (S-PVA) were prepared and the effects of these substitutions on the plasma protein adsorption were studied by one-and two-dimensional gel electrophoresis and immunoblotting. When Cuprophane was used as a negative control, the amount of total proteins bound to samples decreased in the order Cuprophane * PVA * C-PVA * S-PVA, which we attribute to the effects of the surface characteristics of the samples, such as their surface tensions and electrostatic properties, on the adsorption of proteins to the surfaces of the materials. The results revealed that albumin was the most abundant protein in all the samples. The proportion of adsorbed fibrinogen to S-PVA exceeded those of PVA and C-PVA, whereas S-PVA exhibited the lowest IgG adsorption affinity among the samples we studied.

show abstract

On the binding of complement to solid artificial surfaces in vitro

Cited by 54 publications

References 46 publications

Immune complement activation is attenuated by surface nanotopography

Immune complement activation is attenuated by surface nanotopography

Toll like receptor 2/1 mediated platelet adhesion and activation on bacterial mimetic surfaces is dependent on src/Syk-signaling and purinergic receptor P2X1 and P2Y12 activation

Plasma protein adsorption to anion substituted poly(vinyl alcohol) membranes

Contact Info

Product

Resources

About