In this work, we establish a connection between the extended Prelle-Singer procedure with five other analytical methods which are widely used to identify integrable systems in the contemporary literature, especially for second-order nonlinear ordinary differential equations (ODEs). By synthesizing these methods, we bring out the interplay between Lie point symmetries, λ-symmetries, adjoint symmetries, null-forms, Darboux polynomials, integrating factors and Jacobi last multiplier in identifying the integrable systems described by second-order ODEs. We also give new perspectives to the extended Prelle-Singer procedure developed by us. We illustrate these subtle connections with the modified Emden equation as a suitable example.