On the control of molecular weight distribution of polyhydroxybutyrate in Azohydromonas lata cultures

Penloglou, Giannis; Kretza, Eirini; Chatzidoukas, Christos; Parouti, Sophia; Kiparissides, Costas

doi:10.1016/j.bej.2011.12.013

Cited by 32 publications

(30 citation statements)

References 42 publications

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“…The molar mass distribution was unimodal for all procedures except PHB-R4*, for which it was bimodal. This difference is similar to that reported by Penloglou et al [22] when long sonication times were applied to recover PHB from Azohydromonas lata cells. Severe conditions lead to mechanical breakage of polymer chains as a consequence of the high energy input [22].…”

Section: Product Propertiessupporting

confidence: 87%

Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures

López-Abelairas

García‐Torreiro

Lú-Chau

et al. 2015

Biochemical Engineering Journal

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Section: Product Propertiessupporting

confidence: 87%

Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures

López-Abelairas

García‐Torreiro

Lú-Chau

et al. 2015

Biochemical Engineering Journal

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“…In this case, the highest values of M w -2.5 9 10 3 kDa was recorded at C/N ratio of 20 which declined 20 times at C/N ratio of 6. Similarly, M w -2.0 9 10 3 kDa observed at C/P ratio of 8 also declined three times at C/P ratio of 0.8 [76]. In contrast to most studies, where N limitation seems to favour PHA production, recent work has revealed that Bacillus spp.…”

Section: Culture Conditionsmentioning

confidence: 83%

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

et al. 2015

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Polyhydroxyalkanoates (PHAs) as an alternative to synthetic plastics have been gaining increasing attention. Being natural in their origin, PHAs are completely biodegradable and eco-friendly. However, consistent efforts to exploit this biopolymer over the last few decades have not been able to pull PHAs out of their nascent stage, inspite of being the favorite of the commercial world. The major limitations are: (1) the high production cost, which is due to the high cost of the feed and (2) poor thermal and mechanical properties of polyhydroxybutyrate (PHB), the most commonly produced PHAs. PHAs have the physicochemical properties which are quite comparable to petroleum based plastics, but PHB being homopolymers are quite brittle, less elastic and have thermal properties which are not suitable for processing them into sturdy products. These properties, including melting point (T m ), glass transition temperature (T g ), elastic modulus, tensile strength, elongation etc. can be improved by varying the monomeric composition and molecular weight. These enhanced characteristics can be achieved by modifications in the types of substrates, feeding strategies, culture conditions and/or genetic manipulations.

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“…The thermo‐mechanical properties (elasticity, tensile strength and crystallinity) of PHB depend on its weight average molecular weight ( M w ) . The PHB of low M w (<1000 kDa) is more brittle and less elastic, compared with PHB of high M w (≥1000 kDa) or ultra‐high M w (≥4000 kDa) .…”

Section: Introductionmentioning

confidence: 99%

Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight byAzotobacter vinelandiiin batch and fed-batch cultures

Castillo

Flores

Segura

et al. 2017

J. Chem. Technol. Biotechnol

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BACKGROUND Polyhydroxybutyrate (PHB) is a biopolymer with thermo‐mechanical properties like those of plastics produced by the petrochemical industry. These properties are improved in PHBs of high or ultra‐high weight average molecular weight. Azotobacter vinelandii OPNA is a potential producer of large quantities of PHB, having ultra‐high molecular weight by fed‐batch production strategies. RESULTS In batch and fed‐batch cultures at different carbon–nitrogen molar ratios (10, 14 and 18), the OPNA strain produced PHB of high and ultra‐high weight average molecular weight, with values between 2.3 and 6.6 MDa. In this strain, the weight average molecular weight was highly dependent on the initial carbon–nitrogen ratio, reaching the highest value (6.6 MDa) in batch cultures conducted with a carbon–nitrogen ratio of 18 and the lowest (2.3) with a carbon–nitrogen ratio of 10. On the other hand, using a two feeding‐pulses fed‐batch strategy, the highest global PHB volumetric productivity (0.56 g L−1 h−1) and polymer concentration (27.6 g L−1) were obtained. CONCLUSION The OPNA strain accumulated up to 80% of PHB of ultra‐high molecular weight as a function of the initial carbon–nitrogen molar ratio. Using a simple fed‐batch strategy of two feeding‐pulses was possible to produce up to 27 g L−1 of PHB. © 2016 Society of Chemical Industry

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On the control of molecular weight distribution of polyhydroxybutyrate in Azohydromonas lata cultures

Cited by 32 publications

References 42 publications

Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures

Comparison of several methods for the separation of poly(3-hydroxybutyrate) from Cupriavidus necator H16 cultures

Challenges and Opportunities for Customizing Polyhydroxyalkanoates

Production of polyhydroxybutyrate (PHB) of high and ultra-high molecular weight byAzotobacter vinelandiiin batch and fed-batch cultures

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