Bubble size and its distribution are the important parameters which have a direct impact on mass transfer in bubble column reactors. For this, a new robust image processing technique was presented for investigating hydrodynamic aspects and bubble behavior in real chemical or biochemical processes. The experiments were performed in a small-scale bubble column. The study was conducted for the wide range of clear liquid heights and superficial gas velocities. However, a major challenge in image analysis techniques is identification of overlapping or cluster bubbles. This problem can be overcome with the help of the proposed algorithm. In this respect, large numbers of videos were recorded using a high-speed camera. Based on detailed experiments, the gas–liquid dispersion area was divided into different zones. A foam region width was found as inversely proportional to the clear liquid height. An entry region width was found as directly proportional to the clear liquid height. Hydrodynamic parameters, including gas holdup, bubble size distribution, and Sauter mean bubble diameter were evaluated and compared for different operating conditions. The gas holdup was calculated from both height measurement and pixel intensity methods, and it was found to be indirectly proportional to clear liquid height. Bubble sizes affect the bubble column performance; therefore, bubbles are tracked to calculate the bubble size distribution. Experimental results proved that the proposed scheme is robust.