On the diagnostics of Sarcocystis in cattle: sarcocysts of a species unusual for Bos taurus in a dwarf zebu

Odening, K.; Stolte, M.; Bockhardt, I.

doi:10.1016/s0304-4017(96)00993-4

Cited by 33 publications

(24 citation statements)

References 7 publications

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“…According to these authors (with the exception of Erber et al, 1978 who did not use transmission electron microscopy), S. gracilis is characterized by a thin cyst wall with stubby or mushroom-like villar protrusions representing a new ultrastructural cyst wall type compared with the classi®cation by Dubey, Speer & Fayer (1989). Cattle are usually parasitized by S. cruzi, S. hirsuta, and S. hominis (see Dubey et al, 1989;Odening et al, 1995Odening et al, , 1996. Before our description of sarcocysts, which were morphologically indistinguishable from S. gracilis in the European badger (Odening et al, 1994) and in the dwarf zebu , this species was not known from mammals other than the roe deer, though S. tarandivulpes from the reindeer is very similar.…”

Section: Introductionmentioning

confidence: 93%

Scanning electron microscopic identification of Sarcocystis gracilis from roe deer and cattle

Stolte¹,

Bockhardt²,

Odening³

1998

Journal of Zoology

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Sarcocysts morphologically not distinguishable from Sarcocystis gracilis of roe deer (Capreolus capreolus, Cervidae) were found in the skeletal musculature of dwarf zebu and humpless cattle (Bos taurus, Bovidae). We contribute to the identi®cation of this species by means of scanning electron microscopy. Special attention is paid to the species-speci®c cyst wall structure. Our ®ndings con®rm previous descriptions by light and transmission electron microscopy and support the peculiarity and uniqueness of the cyst wall typè gracilis'.

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Section: Introductionmentioning

confidence: 93%

Scanning electron microscopic identification of Sarcocystis gracilis from roe deer and cattle

Stolte¹,

Bockhardt²,

Odening³

1998

Journal of Zoology

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“…Cysts were isolated from dissections using sterile needles. Fresh smear preparations by squash and digestive techniques were applied for the detection and verification of microscopic sarcocysts and existence of bradyzoites (Dubey et al 1989a, b;Odening et al 1996).…”

Section: Sample Collectingmentioning

confidence: 99%

Ultrastructural and molecular characterization of Sarcocystis isolated from camel (Camelus dromedarius) in Iran

et al. 2010

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Sarcocystis cameli was first described in one-humped camels (Camelus dromedarius), and it is the only species which have so far reported in camels. Although more than 150 species of Sarcocystis were described in various animals, only a few data on camel Sarcocystis ultrastructure were published, and this report is the first for molecular information (DNA sequence and RLFP digestion pattern). The main objective of the present work is to characterize Sarcocystis isolated from camels by electron microscopy and PCR-RFLP methods. Muscle samples were taken from the fresh esophagus, diaphragm, skeletal muscles, and heart of one-humped camels (C. dromedarius) slaughtered in abattoirs of Tehran and Ghazvin provinces, Iran. The dissection and trypsin digestion techniques were applied for the detection of the cysts. The infected samples were fixed in glutaraldehyde and/or frozen at -20°C until use for ultrastructural and molecular studies, respectively. The ultrastructural and molecular studies were carried out contemporaneously. The 18S rRNA gene of the parasites was amplified by PCR. The PCR products were cloned into a pTZ57R/T and sequenced. In addition, the PCR products were digested separately with each of the four restriction enzymes for RFLP. Our results indicated that only microcysts were observed in muscle samples. The microcysts were white, elongated, spindled, and a few spiral-shaped, with mean size 260 × 75 μm which are identical with S. cameli. The ultrastructure of microcyst wall had many non-branched finger-like protrusions irregularly folded. There was a 600-bp specific band amplified after PCR with specific primers. The molecular data for camel Sarcocystis is reported for the first time in Iran and the world.

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“…It has been generally assumed that a given species of Sarcocystis is specific to a single intermediate host although exceptions to this have been established through experimental infections and genetic analysis (Dubey et al, 1989;Li et al, 2002;Yang et al, 2001aYang et al, ,b, 2002. Of those Sarcocystis species infecting domestic animals, a given species usually infects only one species of intermediate host and uses either felids or canids, but not both, as its definitive host (Huong et al, 1997;Odening et al, 1995Odening et al, , 1996Zuo et al, 1995).…”

Section: Introductionmentioning

confidence: 97%

Sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings

Chen

Zuo

Rosenthal

et al. 2011

Veterinary Parasitology

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On the diagnostics of Sarcocystis in cattle: sarcocysts of a species unusual for Bos taurus in a dwarf zebu

Cited by 33 publications

References 7 publications

Scanning electron microscopic identification of Sarcocystis gracilis from roe deer and cattle

Scanning electron microscopic identification of Sarcocystis gracilis from roe deer and cattle

Ultrastructural and molecular characterization of Sarcocystis isolated from camel (Camelus dromedarius) in Iran

Sarcocystis sinensis is an ultrastructurally distinct parasite of water buffalo that can cause foodborne illness but cannot complete its life-cycle in human beings

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