On the Effect of a Polypeptide Isolated from “Kalata‐Kalata” (<i>Oldenlandia affinis DC</i>) on the Oestrogen Dominated Uterus

Gran, L

doi:10.1111/j.1600-0773.1973.tb01541.x

Cited by 226 publications

(176 citation statements)

References 5 publications

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“…[4] It is believed that the compact CCK motif of cyclotides is responsible for their exceptional resistance against chemical, thermal, and proteolytic degradation. [5] Cyclotides display a diverse range of biological activities, including uterotonic, [6] haemolytic, [7][8][9] neurotensin antagonistic, [10] HIV inhibitory, [11] antimicrobial, [12] cytotoxic, [13] antifouling, [14] and trypsin inhibitory activities. [15] In addition, kalata B1 and B2 have been shown to inhibit the growth and development of Helicoverpa punctigera and H. armigera larvae, suggesting that cyclotides function as insecticidal agents as part of plant defence systems.…”

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confidence: 99%

Structure and Activity of the Leaf-Specific Cyclotide vhl-2

et al. 2010

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Cyclotides are plant-derived macrocyclic peptides with potential applications in the pharmaceutical and agricultural industries. In addition to their presumed natural function as host-defence peptides arising from their insecticidal activity, their other biological activities include antimicrobial, haemolytic, and cytotoxic activities, but at present, only limited information is available on the structural and chemical features that are important for these various activities. In the current study, we determined the three-dimensional structure of vhl-2, a leaf-specific cyclotide. Although the characteristic cyclic cystine knot fold of other cyclotides is maintained in vhl-2, it has more potent haemolytic activity than well-characterized cyclotides such as kalata B1 and kalata B8. Analysis of surface hydrophobicity and haemolytic activity for a range of cyclotides indicates a correlation between them, with increasing hydrophobicity resulting in increased haemolytic activity. This correlation is consistent with membrane binding being a vital step in mediating the various cytotoxic activities of cyclotides. The gene sequence for vhl-2 was determined and indicates that vhl-2 is processed from a multidomain precursor protein that also encodes the cyclotide cycloviolacin H3.

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confidence: 99%

Structure and Activity of the Leaf-Specific Cyclotide vhl-2

et al. 2010

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“…This is particularly important for the development of peptidebased therapeutics with oral bioavailability. In fact, the use of cyclotide-containing plants in indigenous medicine first highlighted the fact that the peptides are resistant to boiling and are apparently orally bioavailable [14,32,33]. Some cyclotides have also been shown to cross the cell membrane [21] thus allowing to target intracellular protein interactions, such as that mediated by viral FLIPs to prevent cell-death in virally infected cells (unpublished results).…”

Section: Summary and Concluding Remarksmentioning

confidence: 99%

“…The first cyclotide discovered, kalata B1, was identified in the plant Oldenlandia affinis in central Africa in the 1960's [32]. This plant was used by the natives to make a tea extract that was used to accelerate childbirth during labor [14,33].…”

Section: Biological Activities Of Naturally Occurring Cyclotidesmentioning

confidence: 99%

Biological Activities of Natural and Engineered Cyclotides, a Novel Molecular Scaffold for Peptide-Based Therapeutics

Garcia¹,

Camarero²

2010

CMP

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Cyclotides are a growing family of large plant-derived backbone-cyclized polypeptides (≈30 amino acids long) that share a disulfide-stabilized core characterized by an unusual knotted structure. Their unique circular backbone topology and knotted arrangement of three disulfide bonds makes them exceptionally stable to thermal, chemical, and enzymatic degradation compared to other peptides of similar size. Currently more than 100 sequences of different cyclotides have been characterized and the number is expected to increase dramatically in the coming years. Considering their stability, biological activities and ability to cross the cell membrane, cyclotides can be exploited to develop new peptide-based drugs with high potential for success. The cyclotide scaffold can be engineered or evolved using molecular evolution to inhibit proteinprotein interactions implicated in cancer and other human diseases, or design new antimicrobial. The present review reports the biological diversity and therapeutic potential of natural and engineered cyclotides.

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“…Kalata B2 (net charge -1) is generally taken as the prototypic member of AHDPs in cylotides [23,27] and was first reported in the early 1970s when it was identified in Oldenlandia affinis of the Rubiaceae (Table 1), which is widely dispersed over tropical Africa and is also found in tropical Asia [56][57][58]. The peptide derives its name from the fact that it is a component of the uterotonic agent in a medicinal preparation, kalata-kalata [58,59], which is derived from O. affinis and is used in parts of Africa to accelerate child birth [60,61].…”

Section: Ahdps From Cyclotidesmentioning

confidence: 99%

Anionic Host Defence Peptides from the Plant Kingdom: Their Anticancer Activity and Mechanisms of Action

Harris¹,

Prabhu²,

Dennison³

et al. 2016

PPL

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Abstract:It is becoming increasingly clear that plants ranging across the plant kingdom produce anionic host defence peptides (AHDPs) with potent activity against a wide variety of human cancers cells. In general, this activity involves membrane partitioning by AHDPs, which leads to membranolysis and / or internalization to attack intracellular targets such as DNA. Several models have been proposed to describe these events including: the toroidal pore and Shai-Matsuzaki-Huang mechanisms but, in general, the mechanisms underpinning the membrane interactions and anticancer activity of these peptides are poorly understood. Plant AHDPs with anticancer activity can be conveniently discussed with reference to two groups: cyclotides, which possess cyclic molecules stabilized by cysteine knot motifs, and other ADHPs that adopt extended andhelical conformations. Here, we review research into the anticancer action of these two groups of peptides along with current understanding of the mechanisms underpinning this action.

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On the Effect of a Polypeptide Isolated from “Kalata‐Kalata” (Oldenlandia affinis DC) on the Oestrogen Dominated Uterus

Cited by 226 publications

References 5 publications

Structure and Activity of the Leaf-Specific Cyclotide vhl-2

Structure and Activity of the Leaf-Specific Cyclotide vhl-2

Biological Activities of Natural and Engineered Cyclotides, a Novel Molecular Scaffold for Peptide-Based Therapeutics

Anionic Host Defence Peptides from the Plant Kingdom: Their Anticancer Activity and Mechanisms of Action

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