On the formation of the TPN requiring glyceraldehyde-3-phosphate dehydrogenase during the production of chloroplasts in Euglena gracilis

Brawerman, George; Konigsberg, Nancy

doi:10.1016/0006-3002(60)90462-5

Cited by 64 publications

(17 citation statements)

References 13 publications

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“…Do, a measure of sensitivity of the UV targets (17), was 4.5 sec for the wild type organism and 3.5 sec for Y9 cells. Exposure of (4,5,11,24,26) we observed the gradual appearance of these enzyme activities in dark-grown wild type cells exposed to light. The time course for the appearance of these activities over a 48-to 72-hr period and the finding that cycloheximide (15 ,g/ml) inhibited 80 to 95% of the light-induced formation of each of the four proteins strongly support the notion that each enzyme is synthesized de novo during chloroplast development in Euglena.…”

Section: Resultsmentioning

confidence: 88%

“…Brawerman and Konigsberg (5) advanced the hypothesis that photosynthetically generated NADPH might serve as an inducer of this enzyme in Euglena, and more recently Ben-Amotz and Avron (2) demonstrated that disalicylidenepropanediamine (DSPD), a potent antagonist of ferredoxin, prevented the light-induced formation of NADPH glyceraldehyde-3-P dehydrogenase in Euglena. The results reported in this paper render the Brawerman hypothesis extremely unlikely.…”

Section: Resultsmentioning

confidence: 99%

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Light-induced Enzyme Formation in a Chlorophyll-less Mutant of Euglena gracilis

Russell¹,

Draffan²,

Schmidt³

et al. 1978

Plant Physiol.

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A mutant strain, Y,, of Euglena gracilis strain Z that is unable to produce protochlorophyUl or chlorophyl has been isolated folowing treatment The light-induced formation of chloroplasts in Euglena gracilis represents de novo synthesis ofthe several enzymes of the reductive pentose-P cycle, various components of the photosynthetic electron transport chain, chloroplast pigments (Chl a and b, carotenoids), several classes of RNA and other macromolecules, DNA (29,30), and the complex molecular architecture of the mature plastid (22,26). The control mechanisms regulating light-induced plastid differentiation are poorly understood, although several lines of investigation indicate a highly organized sequence of events (13, 19,23,25,26). In an effort to understand better the regulatory mechanisms underlying chloroplast development in Euglena, we have isolated a series of mutant strains blocked at various stages of the developmental process. We report here the characteristics of Y9ZNalL, a mutant unable to synthesize Chl. MATERIALS AND METHODSOrganism and Growth Conditions. E. gracilis var. bacillaris, strain Z, was cultured in acidic organotrophic medium (12) at 26 C. Illumination was provided by 30-w Sylvania cool-white fluorescent bulbs. The light intensity at the surface of the growth flasks was approximately 400 ft-c.

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Section: Resultsmentioning

confidence: 88%

Section: Resultsmentioning

confidence: 99%

Light-induced Enzyme Formation in a Chlorophyll-less Mutant of Euglena gracilis

Russell¹,

Draffan²,

Schmidt³

et al. 1978

Plant Physiol.

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“…When the extract was examined after being frozen 24 hours, the activity was now inhibited by cysteine, EDTA and p-CMB but stimulated by K+. In contrast to spinach and Chondrus the activity of the (4), mutants of Euglena (1,3) or where the cultuiring conditions were of such a nature that respiration either wholly or partially supplants energy derived from photosynthesis for growth including dark grown Euglena and Chlorella (3), the DPN-linked dehydrogenase occurs solely or has a higher specific activity with respect to protein or chlorophyll over the TPN-enzyme. In general, these materials possess Type I and Type II aldolases but the latter appear to be prevalent.…”

Section: Resultsmentioning

confidence: 99%

Role of Aldolase in Photosynthesis. II Demonstration of Aldolase Types in Photosynthetic Organisms

Willard¹,

Gibbs²

1968

Plant Physiol.

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“…10). The formation of some proteins associated with photosynthesis parallels the formation of chlorophyll during greening of etiolated cells (4,16). Thus, the relationship between chlorophyll formation and amino acid-incorporating activitys suggests that the ability of plastids to synthesize protein increases during the period of most active plastid growth and differentiation and again decreases when growth and differentiation are completed.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris

Drumm

Margulies

1970

Plant Physiol.

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Etioplasts are converted to chloroplasts when etiolated leaves of higher plants are placed in the light. Chlorophyll is formed during this conversion, the ability to photosynthesize develops (2,8,19,26,31), and the plastids increase in size and form their characteristic lamellar structures (9,24). Development of the ability to photosynthesize depends both on the formation of chlorophyll and on the formation of protein (11,20,21 tine were used throughout. They were grown in darkness for 6 to 8 days, or they were grown in darkness for 6 days and then illuminated for up to 48 hr. The temperature in darkness was 25 C and in light 28 C. Plants were illuminated with 1000 ft-c of white fluorescent light. Otherwise conditions of growth were the same as described previously (25).Preparation of Plastids. Chloroplasts and etioplasts were prepared from plants that were 6.5 days old, except where mentioned otherwise. Preparation of etioplasts was carried out in dim green light (5). Otherwise, both etioplasts and chloroplasts were prepared in the same way. Leaves were picked and stored and plastids were isolated as described previously (25)

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On the formation of the TPN requiring glyceraldehyde-3-phosphate dehydrogenase during the production of chloroplasts in Euglena gracilis

Cited by 64 publications

References 13 publications

Light-induced Enzyme Formation in a Chlorophyll-less Mutant of Euglena gracilis

Light-induced Enzyme Formation in a Chlorophyll-less Mutant of Euglena gracilis

Role of Aldolase in Photosynthesis. II Demonstration of Aldolase Types in Photosynthetic Organisms

In Vitro Protein Synthesis by Plastids of Phaseolus vulgaris

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