On the genetic activity of benzimidazole and thiophanate fungicides on diploid aspergillus nidulans

Mitotic Arrest and Chromosome Doubling Using Thiabendazole, Cambendazole, Nocodazole and Ben Late in the Slime Mould Dictyostelium discoideum

“…While benomyl and MBC have been reported to induce point mutations in Salmonella typhimurium (Seiler, 1972 ;Kappas et al, 1976), the induction of point mutations was not observed in A. nidulans (Hastie, 1970;Kappas et al, 1974;Kappas, 1978), Saccharomyces cerevisiae (Siebert et al, 1970) or mammalian cells (Seiler, 1975). Likewise, we have not detected changes in the frequency of developmental mutants in D. discoideurn following treatment with any of the benzimidazole derivatives; their major genetic effect on D. discoideum appears to be the induction of non-disjunction.…”

“…Present evidence indicates that the major genetic effect of benomyl and thiabendazole on Aspergillus nidulans is the induction of non-disjunction (Hastie, 1970;Kappas et al, 1974;Kappas, 1978). While benomyl and MBC have been reported to induce point mutations in Salmonella typhimurium (Seiler, 1972 ;Kappas et al, 1976), the induction of point mutations was not observed in A. nidulans (Hastie, 1970;Kappas et al, 1974;Kappas, 1978), Saccharomyces cerevisiae (Siebert et al, 1970) or mammalian cells (Seiler, 1975).…”

In Vitro Cytogenetic Effects of Agrizim on Human Lymphocytes with and without Metabolic Activation.

“…The DNA alterations caused by the carbendazim have been suspected to be induced by the presence of benzimidazole moiety which is a base analogue of purine and is likely to be incorporated into DNA by misrepair of gaps between the newly synthesized DNA (Kappas 1981). While Seiler (1972) reported methyl benzimidazole carbamate (MBC) to be mutagenic in Salmonella typhimurium by acting as base substituting agent, Pandita (1988) reported its non-mutagenicity in the same system both in the absence and presence of S9 mix.…”

“…Carbendazime, methyl-2-benzimidazole carbamate (MBC) and its active metabloites, have been reported to induce gene mutations in Salmonella typhimurium (Seiler 1972), Escherichia coli (Kappas et al 1976), Fusarium oxysporum (Dassenoy and Meyer 1973); clastogenic mani festations in Allium cepa, A. sativum and Vicia faba (Sahu et al 1983), Coffea arabica (Soma shekar et al 1983), mouse bone marrow (Tsuchimoto and Matter 1977), Chinese hamster bone marrow (Seiler 1976(Seiler , 1977 and human cells in vitro (Styles andGarner 1974, De Brabander et al 1976). However, some later reports have been contradictory.…”

Analysis of Mitotic Nondisjunction with Aspergillus nidulans

“…The action of some other compounds is described elsewhere (5,6). The data from other laboratories derive from Kappas, Georgopulos and Hastie (8,9). Very recently we tested one other polyene antibiotic, pymaricin, which efficiently induces nondisjunction.…”