On the genus <i>Bursaphelenchus</i> Fuchs, 1937 (Nematoda: Parasitaphelenchinae) associated with wood and insects from declining forest trees in the Czech Republic

Čermák, Václav; Vieira, Paulo; Gaar, V.; Čudejková, Mária; Foit, Jiří; Zouhar, Miloslav; Douda, Ondřej; Mota, Manuel

doi:10.1111/efp.12031

Cited by 19 publications

(3 citation statements)

References 22 publications

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“…In the same survey, potential interactions between PWN and O. erosus , O. laricis and P. bidentatus were also investigated. Even though these beetles are reported in the literature as possible vectors of several nematode species (Čermák et al., 2013; Holuša, Lukášová, Hubáčková, Knížek, & Wegensteiner, 2017; Robertson et al, 2008; Torrini et al., 2017), no associations with PWN or any other organism were observed in our study. These findings could be simply due to the small sample of size of the collected species that strongly reduced the chance of detecting potentially associated organisms.…”

Section: Discussioncontrasting

confidence: 68%

First record of Ektaphelenchoides pini associated with Ips sexdentatus on Pinus nigra laricio in Italy

et al. 2020

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In February 2015, an unexpected windstorm downed five hectares of a European black pine Pinus nigra subsp. laricio forest formation located close to Vallombrosa, Florence (Central Italy). In the following spring, an extensive survey was conducted in the area. Felled trees, stumps and all the suitable plant material were screened for the presence of the pinewood nematode (PWN), Bursaphelenchus xylophilus, by sampling wood and bark. Bark beetles were then collected from the gallery systems on the inner side of bark samples and observed in the laboratory. The following bark beetles were morphologically identified: Ips sexdentatus, Orthotomicus erosus, O. laricis and Pityogenes bidentatusa. The dissection of Ips sexdentatus allowed the extraction of numerous nematodes that were morphologically and molecularly identified as Ektaphelenchoides pini. Conversely, only few nematode specimens were isolated from either pine bark or wood. These individuals could be only molecularly identified and belonged to an undescribed nematode taxon. Even though no PWN was recorded in the investigated sites, our survey allowed the detection of a new association between E. pini and I. sexdentatus on P. nigra.

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Section: Discussioncontrasting

confidence: 68%

First record of Ektaphelenchoides pini associated with Ips sexdentatus on Pinus nigra laricio in Italy

et al. 2020

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“…Bursaphelenchus vallesianus was first detected and described in declining pine stands in Canton Valais during the survey conducted between 2002 and 2005 (Braasch et al ., ; Polomski et al ., ). This species has been successively isolated from black pines ( P. nigra ) in Greece (Lange et al ., ), and from Scots pines in Turkey (Akbulut et al ., ) and in the Czech Republic (Čermák et al ., ). Bursaphelenchus sexdentati has been detected in several Swiss cantons at elevations up to 1700 m a.s.l.…”

Section: Discussionmentioning

confidence: 97%

“…These species have occasionally been detected in coniferous forests (mainly Scots pine) in central and northern Europe (e.g. Braasch, ; Čermák et al ., ), but their ecological role is still unclear. The identity of B. leoni , however, could not be confirmed by genetic analysis because of the lack of reference sequences.…”

Section: Discussionmentioning

confidence: 99%

Occurrence and ITS diversity of wood‐associatedBursaphelenchusnematodes in Scots pine forests in Switzerland

2015

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The quarantine pathogen Bursaphelenchus xylophilus (pine wood nematode, PWN) represents a serious threat for Pinus species in Europe. To exclude its presence in Switzerland, in 2010 and 2011 a countrywide survey was conducted in 102 Pinus sylvestris stands, chosen according to whether they contained dying or dead trees or were located in areas at risk of PWN introduction. In total, 285 trees (1–5 per site) were sampled. Nematodes were extracted from wood chips using a standard procedure, and identified to species by internal transcribed spacer (ITS) sequencing. Bursaphelenchus species were present in 34% of the trees, but no B. xylophilus was identified, i.e. PWN is still not present in Switzerland. The nematodes found belonged to seven different species, with B. vallesianus the most frequent species, followed by B. sexdentati, B. mucronatus kolymensis and B. eggersi. Three other species (B. borealis, B. pinophilus, B. poligraphi) were each only present in one or two trees. Three groups of sequences could not be assigned to a species because of the lack of matching reference sequences. The species composition found in Switzerland suggests co‐existence of southern and central European Bursaphelenchus species. Intraspecific ITS variability differed considerably among the four most common species. Bursaphelenchus eggersi, B. mucronatus kolymensis and B. sexdentati had several variable sites in the ITS region, resulting in multiple ITS genotypes in each species. In contrast, all 99 B. vallesianus isolates had an identical ITS region. This could indicate a founder effect, and possibly that B. vallesianus is not native to the Alpine region.

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New insights into the life history of Monochamus galloprovincialis can enhance surveillance strategies for the pinewood nematode

et al. 2019

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On the genus Bursaphelenchus Fuchs, 1937 (Nematoda: Parasitaphelenchinae) associated with wood and insects from declining forest trees in the Czech Republic

Cited by 19 publications

References 22 publications

First record of Ektaphelenchoides pini associated with Ips sexdentatus on Pinus nigra laricio in Italy

First record of Ektaphelenchoides pini associated with Ips sexdentatus on Pinus nigra laricio in Italy

Occurrence and ITS diversity of wood‐associatedBursaphelenchusnematodes in Scots pine forests in Switzerland

New insights into the life history of Monochamus galloprovincialis can enhance surveillance strategies for the pinewood nematode

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