On the glandular origin of seminal plasma lipids in man

Vignon, F.; Clavert, A.; Köll-Back, M H; Reville, P

doi:10.1111/j.1439-0272.1992.tb02666.x

Cited by 9 publications

(3 citation statements)

References 13 publications

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“…These lipases provide a potential mechanism to break down neutral lipid transferred in microcarriers to females so the products can be used as fuel. Mammalian seminal fluid also contains lipases (47)(48)(49) and triacylglycerides (50,51), suggesting that the latter may be required, perhaps as a male-derived nutrient source, in the reproductive system of all higher organisms.…”

Section: Discussionmentioning

confidence: 99%

Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid

Wainwright

Hopkins

Mendes

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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Seminal fluid plays an essential role in promoting male reproductive success and modulating female physiology and behavior. In the fruit fly, Drosophila melanogaster, Sex Peptide (SP) is the best-characterized protein mediator of these effects. It is secreted from the paired male accessory glands (AGs), which, like the mammalian prostate and seminal vesicles, generate most of the seminal fluid contents. After mating, SP binds to spermatozoa and is retained in the female sperm storage organs. It is gradually released by proteolytic cleavage and induces several long-term postmating responses, including increased ovulation, elevated feeding, and reduced receptivity to remating, primarily signaling through the SP receptor (SPR). Here, we demonstrate a previously unsuspected SPR-independent function for SP. We show that, in the AG lumen, SP and secreted proteins with membrane-binding anchors are carried on abundant, large neutral lipid-containing microcarriers, also found in other SP-expressing Drosophila species. These microcarriers are transferred to females during mating where they rapidly disassemble. Remarkably, SP is a key microcarrier assembly and disassembly factor. Its absence leads to major changes in the seminal proteome transferred to females upon mating. Males expressing nonfunctional SP mutant proteins that affect SP’s binding to and release from sperm in females also do not produce normal microcarriers, suggesting that this male-specific defect contributes to the resulting widespread abnormalities in ejaculate function. Our data therefore reveal a role for SP in formation of seminal macromolecular assemblies, which may explain the presence of SP in Drosophila species that lack the signaling functions seen in D. melanogaster.

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Section: Discussionmentioning

confidence: 99%

Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid

Wainwright

Hopkins

Mendes

et al. 2021

Proc. Natl. Acad. Sci. U.S.A.

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“…Cholesterol is present in seminal plasma and has been suggested to originate from the prostate (Eliasson, ), the seminal vesicles (Scott et al ., ), serum, and lymph (Jones, ). Investigations of split ejaculates and whole ejaculates from vasectomized and non‐vasectomized patients supported that cholesterol in seminal plasma primarily originates from the prostate (Vignon et al ., ). In line with this finding, it has been suggested that cholesterol is released from prostasomes (Ronquist & Brody, ; Kravets et al ., ).…”

Section: Introductionmentioning

confidence: 97%

Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross‐sectional cohort study and immunohistochemical investigations

et al. 2018

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“…These lipases provide a potential mechanism to break down neutral lipid transferred in microcarriers to females, so the products can be used as fuel. Mammalian seminal fluid also contains lipases (40, 41, 42) and triacylglycerides (43, 44), suggesting that the latter may be required, perhaps as a male-derived nutrient source, in the reproductive system of all higher organisms.…”

Section: Discussionmentioning

confidence: 99%

DrosophilaSex Peptide Controls the Assembly of Lipid Microcarriers in Seminal Fluid

Wainwright

Mendes

Sekar

et al. 2020

Preprint

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20 Seminal fluid plays an essential role in promoting male reproductive success and modulating 21 female physiology and behaviour. In the fruit fly, Drosophila melanogaster, Sex Peptide (SP) 22 40 41 Significance Statement 42 Seminal fluid plays a critical role in reprogramming female physiology and behaviour to 43 promote male reproductive success. We show in the fruit fly that specific seminal proteins, 44 including the archetypal 'female-reprogramming' molecule Sex Peptide, are stored in male 45 seminal secretions in association with large neutral lipid-containing microcarriers, which 46 rapidly disperse in females. Related structures are also observed in other Sex Peptide-47 expressing Drosophila species. Males lacking Sex Peptide have structurally defective 48 microcarriers, leading to abnormal cargo loading and transfer to females. Our data reveal 49 that this key signalling molecule in Drosophila seminal fluid is also a microcarrier assembly 50 factor that controls transfer of other seminal factors, and that this may be a more 51 evolutionarily ancient role of this protein. 52 53 54

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On the glandular origin of seminal plasma lipids in man

Cited by 9 publications

References 13 publications

Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid

Drosophila Sex Peptide controls the assembly of lipid microcarriers in seminal fluid

Positive association between cholesterol in human seminal plasma and sperm counts: results from a cross‐sectional cohort study and immunohistochemical investigations

DrosophilaSex Peptide Controls the Assembly of Lipid Microcarriers in Seminal Fluid

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