On the Heterogeneity of Three-Times-crystallized α-Chymotrypsin^*

Mechanism of chymotrypsin hydrolysis of an ester and an anilide

“…Aliquots were withdrawn at various times and titrated directly with CI. A plot of log (% CI activity remaining) versus time of reaction was obviously biphasic, with a component representing 29% of the initial enzyme losing activity with a rate constant k = 0.086 min-' , and the rest of the enzyme losing activity with a rate constant k = 0.0045 min-' , Similar plots were reported by Erlanger et al [8] for the thermal denaturation of chymotrypsin.…”

Consumption of diets containing raw soya beans (Glycine max), kidney beans (Phaseolus vulgaris), cowpeas (Vigna unguiculata) or lupin seeds (Lupinus angustifolius) by rats for up to 700 days: effect on body composition and organ weights

“…The level of uninhibited trypsin activity in the reaction mixture was assessed using N a-benzoyl-DLarginine-p-nitroanilide as substrate (Kakade et al 1969) and the level of uninhibited chymotrypsin was evaluated using glutaryl-1-phenylalanine-p-nitroanilide as substrate (Erlanger et al 1964). Trypsin and chymotrypsin inhibitory activity was expressed as g enzyme inhibited/kg original raw meal and the protease-inhibitor contents of the test diets calculated on the basis of the level of raw seed meal inclusion in the diet.…”

Purification and Some Physical Properties of a Chymotrypsin‐Like Protease of the Larva of the Hornet, Vespa orientalis

“…Chymotrypsin activity was determined photometrically a t 405 nm with gluratyl-L-phenylalaninep-nitroanilide as substrate [2]. The reaction mixture contained 400 p1 4 mM substrate in 0.2 M Tris-HC1 pH 8.…”