Abstract-In this paper, we consider the application of noncoherent Grassmannian signalling in practical multi-channelfrequency-flat multiple-input multiple-output (MIMO) wireless communication systems. In these systems, Grassmannian signalling, originally developed for single-channel block-fading systems, is not readily applicable. In particular, in such systems, the channel coefficients are constant across time and frequency, which implies that spectrally-efficient signalling ought to be jointly structured over these domains. To approach this goal, we develop a concatenation technique that yields a spectrally-efficient time-frequency Grassmannian signalling scheme, which enables the channel coherence bandwidth to be regarded as an additional coherence time. This scheme is shown to achieve the high signalto-noise ratio non-coherent capacity of MIMO channels when the fading coefficients are constant over a time-frequency block. This scheme is also applicable in fast fading systems with coherence bandwidth exceeding that of one subchannel. The proposed scheme is independent of the symbol duration, i.e., the channel use duration, and is thus compatible with the transmit filter designs in current systems.