On the improvement of solid-phase extraction room-temperature phosphorimetry for the analysis of polycyclic aromatic hydrocarbons in water samples

Hagestuen, Erik D.; Arruda, Andréa F.; Campiglia, Andrés D.

doi:10.1016/s0039-9140(00)00427-6

Cited by 49 publications

(38 citation statements)

References 15 publications

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“…As a result, solid surface luminescence analysis is a widely accepted tool in environmental, pharmaceutical, food and agricultural science [3]. Particularly attractive is the use of solid-phase extraction (SPE) membranes for the on-site analysis of polycyclic aromatic compounds in water samples [4][5][6][7][8]. Attractive features include a simple experimental procedure and compatibility with portable instrumentation.…”

Section: Introductionmentioning

confidence: 99%

“…After drying for 5 min, the tabs were examined via front surface room-temperature fluorescence spectroscopy to reach limits of detection (LODs) at the parts-per-billion (ng·mL − 1 ) level [5,13]. Later reports extended the use of SPE membranes to the room temperature phosphorimetry (RTP) analysis of PAHs [6,[14][15][16][17], polychlorinated biphenyls [15,17] and polychlorinated dibenzofurans [17]. Bulky glassware and vacuum pumps, common to classic SPE lab procedures, were replaced with a syringe kit well-suited for manual extraction under field conditions.…”

Section: Introductionmentioning

confidence: 99%

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Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Alfarhani

Al-Tameemi

Schenone

et al. 2016

Microchemical Journal

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a b s t r a c tBenzo[a]pyrene (B[a]P) is a prototypic carcinogenic polycyclic aromatic hydrocarbon (PAH), which requires metabolic activation to produce its detrimental effects. Measurement of B[a]P metabolites in human urine could provide a direct way to assess individual differences in susceptibility to PAH-related cancer. This article focuses on the development of screening methodology for the routine analysis of B[a]P metabolites in urine samples. It explores the solid-surface room-temperature fluorescence (RTF) properties of 3-hydroxybenzo[a]pyrene, benzo[a]pyrene-trans-9,10-dihydrodiol, benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol and benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol previously extracted from urine samples with octadecyl-silica membranes. Relative standard deviations varying from 2.1% (benzo[a]pyrene-r-7,t-8,c-9-tetrahydrotriol) to 8.6% (3-hydroxy-benzo[a]pyrene) are obtained with the aid of fiber optic probe that eliminates the need for manual optimization of signal intensities. Analytical recoveries from human urine samples varied from 87.5 ± 3.1% (3-hydroxy-benzo[a]pyrene) to 99.8 ± 2.5% (benzo[a]pyrene-r-7,t-8,c-9,c-10-tetrahydrotetrol). The excellent analytical figures of merit and the simplicity of the experimental procedure demonstrate the potential of this approach for screening biomarkers of PAH exposure in numerous urine samples.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Alfarhani

Al-Tameemi

Schenone

et al. 2016

Microchemical Journal

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“…The present work represents a new development and application of analytical methods based on the selective adsorption of hydrophobic pollutants on a solid sorbent associated with spectroscopic detection directly applied on the solid substrate [19,20,21,22,23,24,25,26,27,28,29]. The evaluation of the potential of this approach for the analysis of 1-OHPy in human urine demonstrates that the method we propose is very sensitive and can be applied to the biological monitoring of people differently exposed or not to PAH.…”

Section: Discussionmentioning

confidence: 94%

“…Previous works [19,20,21,22,23,24,25,26,27,28,29] have shown that, using appropriate solid sorbent for extraction/concentration, it is possible to avoid the elution required in the solid phase and chromatographic steps by performing in situ spectroscopic detection of benzenic compounds [20], PAH [21,22,23,24,25,26,27] or polychlorobiphenyls [28,29] in aqueous solution directly on the solid phase. Recently, this approach has been proposed for the detection of 1-OHPy in human urine [24].…”

Section: Introductionmentioning

confidence: 99%

Detection of 1-hydroxypyrene in urine by direct fluorometric analysis on a solid sorbing phase. Validation and application of the method to biological monitoring of PAH-exposed persons

Lamotte

Belfutmi²,

Violet

et al. 2003

Analytical and Bioanalytical Chemistry

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A new method for the detection and quantification of 1-hydroxypyrene (1-OHPy) in the urines of persons exposed to polycyclic aromatic hydrocarbons (PAH) has been evaluated. The method is based on extraction/concentration of the analyte onto a small element cut into tabs from an extraction disk (ENVI-Disk trade mark C18 from Supelco) combined with front-face synchronous fluorescence detection and direct quantification on the solid sorbent element. The limit of detection for 1-OHPy was estimated to be about 0.03-0.04 micro g/L, a value which is significantly lower than the pyrene metabolite concentration commonly found in unexposed to weakly PAH-exposed persons ( approximately 0.1-0.3 micro g/L). A quantification based on only one standard addition has been adopted and the method was validated both by testing analyte recovery using a known amount of a commercially available pyrene metabolite and by comparing the results with those obtained by high-performance liquid chromatography (HPLC). The results were very close to the HPLC results, the largest deviations being attributed to variations or defects in the stirring system, the rate stability of which was found to be of major importance for the reproducibility and reliability of the measurements. The applicability of the method was further tested by analyzing 1-OHPy in the urine of a volunteer exposed to various automobile traffic zones. The results confirm previous findings which lead to the conclusion that urinary concentrations of PAH metabolites are influenced more by smoking habit than by exposure to urban atmospheric pollution. Thus, the method appears to be an alternative to the usual method based on HPLC. Moreover it presents some advantages, being simple to operate and requiring relatively low-cost instruments.

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“…Positive pressure was used to force all liquid solutions (5 ml) through the membrane in approximately 30 s per sample. This flow rate is in the optimum range for maximum breakthrough volume (10-30 ml min −1 ) [31]. Following the NX extraction, the sample was dried by forcing two 25 ml volumes of air through the disk using a 25 ml syringe (Hamilton, Reno, NV, USA).…”

Section: Spe-solid-surface Fluorescencementioning

confidence: 99%

Combined liquid and solid-surface room temperature fluorimetric determination of naproxen and salicylate in serum

Ibáñez

Escandar

2005

Journal of Pharmaceutical and Biomedical Analysis

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On the improvement of solid-phase extraction room-temperature phosphorimetry for the analysis of polycyclic aromatic hydrocarbons in water samples

Cited by 49 publications

References 15 publications

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Room temperature fluorescence spectroscopy of benzo[a]pyrene metabolites on octadecyl extraction membranes

Detection of 1-hydroxypyrene in urine by direct fluorometric analysis on a solid sorbing phase. Validation and application of the method to biological monitoring of PAH-exposed persons

Combined liquid and solid-surface room temperature fluorimetric determination of naproxen and salicylate in serum

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