Steel plate shear walls (SPSWs) as a resistant system against lateral loads have a high potential for earthquake energy dissipation. Due to the uncertainties of loading, construction, and installation of SPSWs, it is vital to investigate the importance of each component and achieve higher accuracy in design and the implementation of these members. In this paper, a sensitivity analysis is carried out to determine the significance of important uncertainties. The results denoted that the most important parameters affecting the loading capacity of the SPSWs are height, thickness, length, Young’s modulus of the wall material, flange, and web thickness of the column, respectively.