On the interactions of histone H4 and H4 peptides with DNA. Electrooptical, hydrodynamic and electron microscopy studies

Palau, Jaume; Mozo, Angel; Querol, Enrique

doi:10.1016/s0300-9084(80)80398-1

Cited by 5 publications

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Etude des propriétés électro‐optiques de i' adn par biréfringence électrique a faible champ, 2. Mécanismes d'orientation et de relaxation

et al. 1984

View full text Add to dashboard Cite

The electric birefringence of calf thymus DNA is measured at different low electric field strengths with rectangular and bipolar pulses as a function of concentration, ionic strength and molecular weight, and these results are interpreted in terms of orientation and relaxation mechanisms. The orientation of DNA is mainly due to an induced dipole, but the results reveal a slow induced moment, which is saturated, when low field strengths are applied. A well-defined slow relaxation mechanism is demonstrated, which is attributed to the orientation of DNA as a whole. The long relaxation time decreases when high electric fields or high DNA concentrations are used, and the fact that the decay of the birefringence also contains short relaxation times does not reflect the polydispersity of the samples. The comparison between the molecular lengths determined from long relaxation times and the theoretical lengths of rigid cylinders shows an important coiling of DNA, when the fragments are longer than 110-120 nm.

show abstract

Etude des propriétés électro‐optiques de i' adn par biréfringence électrique a faible champ, 2. Mécanismes d'orientation et de relaxation

et al. 1984

View full text Add to dashboard Cite

show abstract

Etude des propriétés électro‐optiques de l'ADN par biréfringence électrique à faible champ, 4. Influence des interactions ADN‐histones

Marion¹,

Roux²

1985

Makromol. Chem.

View full text Add to dashboard Cite

The interactions of DNA with histone complexes (H2A, H2B, H3, H4) were studied by lowfield electric birefringence measurements. Electro-optical measurements revealed that histones induce a folding of the DNA molecules: with increasing ratio histones/DNA, the steady-state birefringence and the orientational relaxation time decrease gradually. A contribution of a permanent dipole moment to the orientation mechanism appears, while the electrical polarizability decreases quickly without significant differences in the optical anisotropy factors.

show abstract

Evolution of Chromosomal Proteins

Hunt

Dayhoff

1982

Macromolecular Sequences in Systematic and Evolutionary Biology

View full text Add to dashboard Cite

On the interactions of histone H4 and H4 peptides with DNA. Electrooptical, hydrodynamic and electron microscopy studies

Cited by 5 publications

References 20 publications

Etude des propriétés électro‐optiques de i' adn par biréfringence électrique a faible champ, 2. Mécanismes d'orientation et de relaxation

Etude des propriétés électro‐optiques de i' adn par biréfringence électrique a faible champ, 2. Mécanismes d'orientation et de relaxation

Etude des propriétés électro‐optiques de l'ADN par biréfringence électrique à faible champ, 4. Influence des interactions ADN‐histones

Evolution of Chromosomal Proteins

Contact Info

Product

Resources

About