On the mechanics of bacterial biofilms on non-dissolvable surgical sutures: A laser scanning confocal microscopy-based finite element study

Limbert, Georges; Bryan, Rebecca; Cotton, Ross; Young, Paul A.; Hall-Stoodley, Luanne; Kathju, Sandeep; Stoodley, Paul

doi:10.1016/j.actbio.2013.01.017

Cited by 20 publications

(12 citation statements)

References 61 publications

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“…[15] Interestingly, Limbert et al recently conducted finite element analysis predicting biofilm debonding due to substrate micromotion caused by tension, torsion, or bending but did not provide experimental results to confirm their predictions. [22] The current work represents the first experimental observation of active debonding of a mature, crystalline biofilm (>24 hrs old) by strain applied to an elastomeric substrate.…”

Section: Resultsmentioning

confidence: 99%

Soft Robotic Concepts in Catheter Design: an On‐Demand Fouling‐Release Urinary Catheter

Levering

Wang

Shivapooja

et al. 2014

Adv Healthcare Materials

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Infectious biofilms are problematic in many healthcare-related devices, and are especially challenging and ubiquitous in urinary catheters. This report presents an on-demand fouling-release methodology to mechanically disrupt and remove biofilms, and proposes this method for the active removal of infectious biofilms from the previously-inaccessible main drainage lumen of urinary catheters. Mature Proteus mirabilis crystalline biofilms detach from silicone elastomer substrates upon application of strain to the substrate, and increasing the strain rate increases biofilm detachment. The study presents a quantitative relationship between applied strain rate and biofilm debonding through an analysis of biofilm segment length and the driving force for debonding. Based on this mechanism, hydraulic and pneumatic elastomer actuation was used to achieve surface strain selectively within the lumen of prototypes of sections of a fouling-release urinary catheter. Proof-of-concept prototypes of sections of active, fouling-release catheters were constructed using techniques typical to soft robotics including 3D printing and replica molding, and those prototypes demonstrate release of mature P. mirabilis crystalline biofilms (e.g., ≈90%) from strained surfaces. These results provide a basis for the development of a new urinary catheter technology in which infectious biofilms are effectively managed through new methods that are entirely complementary to existing approaches.

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Section: Resultsmentioning

confidence: 99%

Soft Robotic Concepts in Catheter Design: an On‐Demand Fouling‐Release Urinary Catheter

Levering

Wang

Shivapooja

et al. 2014

Adv Healthcare Materials

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show abstract

“…Furthermore, CLSM images have been directly implemented in simulation tools to show the relation between microscopic biofilm structures on the one hand and shear forces on the other (Böl et al, ). Another example is a finite‐element study of Staphylococcus aureus released from a suture by tension, torsion, and bending studied by Limbert et al (). The authors used the CLSM images to feed the model with real observed biofilm structures.…”

Section: Oct In Biofilm Researchmentioning

confidence: 99%

Optical coherence tomography in biofilm research: A comprehensive review

Wagner

Horn

2017

Biotech & Bioengineering

150

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Imaging of biofilm systems is a prerequisite for a better understanding of both structure and its function. The review aims to critically discuss the use of optical coherence tomography (OCT) for the visualization of the biofilm structure as well as its dynamic behavior. A short overview on common and well-known, established imaging techniques for biofilms such as scanning electron microscopy (SEM), confocal laser scanning microscopy (CLSM), Raman microscopy (RM), and magnetic resonance imaging (MRI) paves the way to imaging biofilms at the mesoscale, which is perfectly covered by means of OCT. Principle, resolution, imaging velocity, and limitations of OCT are subsequently presented and discussed in the context of biofilm applications. Examples are provided showing the strength of this technique with respect to the visualization of the mesoscopic biofilm structure as well as the estimation of flow profiles and shear rates. Common and new structural parameters derived from OCT datasets are presented. Additionally, the review shows the importance of OCT with respect to a better description of mechanical biofilm properties. Finally, the implementation of multi-dimensional OCT datasets in biofilm modelling is shown by several examples aiming on an improved understanding of mass transfer at the bulk-biofilm interface. Biotechnol. Bioeng. 2017;114: 1386-1402. © 2017 Wiley Periodicals, Inc.

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“…Image-based modelling has become ubiquitous in many research domains where capturing complex multiphasic structures at various length scales is essential for conducting physics-based numerical analyses [69]. In the context of biological tissues and structures, any type of imaging modalities (1D, 2D, 3D, 4D) can be used including digital optical photography [20], computed tomography [70], magnetic resonance imaging [71] and laser confocal microscopy [72].…”

Section: (B) Classification Of Constitutive Modelsmentioning

confidence: 99%

Mathematical and computational modelling of skin biophysics: a review

Limbert

2017

Proc. R. Soc. A.

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The objective of this paper is to provide a review on some aspects of the mathematical and computational modelling of skin biophysics, with special focus on constitutive theories based on nonlinear continuum mechanics from elasticity, through anelasticity, including growth, to thermoelasticity. Microstructural and phenomenological approaches combining imaging techniques are also discussed. Finally, recent research applications on skin wrinkles will be presented to highlight the potential of physics-based modelling of skin in tackling global challenges such as ageing of the population and the associated skin degradation, diseases and traumas.

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On the mechanics of bacterial biofilms on non-dissolvable surgical sutures: A laser scanning confocal microscopy-based finite element study

Cited by 20 publications

References 61 publications

Soft Robotic Concepts in Catheter Design: an On‐Demand Fouling‐Release Urinary Catheter

Soft Robotic Concepts in Catheter Design: an On‐Demand Fouling‐Release Urinary Catheter

Optical coherence tomography in biofilm research: A comprehensive review

Mathematical and computational modelling of skin biophysics: a review

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