1996
DOI: 10.1016/0014-5793(95)01533-7
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On the mechanism by which 6‐ketocholestanol protects mitochondria against uncoupling‐induced Ca2+ efflux

Abstract: This work shows that 6-ketocholestanol (kCh) inhibits the effect of carbonyl cyanide-m-chlorophenyl hydrazone (CCP) on mitochondrial Ca 2÷ efflux. Such an effect proved to be caused by diminution of membrane fluidity, therefore, it is affected by the incubation temperature. Furthermore, kCh reversed CCPinduced Ca 2+ efflux depending on the accumulation of phosphate. It is also shown that kCh enhances the effect of carboxyatractyloside on membrane permeability transition.

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Cited by 16 publications
(10 citation statements)
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“…3f, no effect on the OCR induced by FR58P1a was observed. Then, to determine whether the uncoupling of mitochondria was mediated by protonophoric action of FR58P1a, we treated the isolated mitochondria with 6-ketocholestanol (6-Keto), a keto-derivative of cholesterol that decreases the membrane fluidity preventing uncoupling mediated by protonophores 27,28 . As shown in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…3f, no effect on the OCR induced by FR58P1a was observed. Then, to determine whether the uncoupling of mitochondria was mediated by protonophoric action of FR58P1a, we treated the isolated mitochondria with 6-ketocholestanol (6-Keto), a keto-derivative of cholesterol that decreases the membrane fluidity preventing uncoupling mediated by protonophores 27,28 . As shown in Fig.…”
Section: Resultsmentioning
confidence: 99%
“…It is conceivable that relocation of PC-TP to mitochondria increases the amount of 16:0/PUFA-PC species in the mitochondrial membranes. The ensuing increase of membrane fluidity is reported to generate a number of responses, such as the enhancement of membrane-associated phospholipase A 2 activity [43], proton leakage [44,45], and Ca 2ϩ efflux [46]. Furthermore, changes in phospholipid fatty acid composition can alter the activity of carnitine palmitoyltransferase I [47,48] and of the pyruvate carrier [49,50].…”
Section: Discussionmentioning
confidence: 99%
“…Submitochondrial particles (SMP) were obtained by sonication of a suspension of thawed out rat liver mitochondria as described [13]. Fluorescence polarization was measured in SMP (1 mg protein/ml of IM) as reported in [10]. Oxygen consumption was recorded with a Clark‐type oxygen electrode.…”
Section: Methodsmentioning
confidence: 99%
“…After the control reading, 6‐kch was added. Calculations were carried out as indicated in [10]. All other experimental conditions are described in the corresponding figure legends.…”
Section: Methodsmentioning
confidence: 99%
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