On the mechanism of arginine requirement for adenovirus synthesis

Abstract

The effects of arginine deprivation on the synthesis and processing of viral proteins and the assembly of incomplete and complete virions were studied during infection with human adenovirus type 2. Arginine deprivation greatly reduced the synthesis of all viral proteins, particularly the precursor to core protein VII. The inhibition was completely reversible by the addition of arginine to the medium. Arginine deprivation between 7 and 20 hours post-infection inhibited the processing of PVII to VII, suggesting that PVII is not cleaved autocatalytically. The assembly of incomplete virions was sensitive to arginine deprivation only prior to 20 hours, while the assembly of complete virions was dependent on the continuous presence of arginine. This observation supports the hypothesis that incomplete virions are precursors of complete virions. The experiments on the PVII-specific endoprotease activity showed that arginine deprivation caused only slight reduction in the in vitro activity, although no activity was observed in vivo. The present results lead to the hypothesis that arginine deficiency inhibits the synthesis of a functional protein essential for virion maturation, other than the synthesis or processing of PVII.

L‐lysine therapy to control the clinical evolution of pityriasis rosea: Clinical case report and literature review

“…One of these amino acids is L ‐arginine, essential for the replication of some viruses and the progression of infections, 45 raising the hypothesis that it would be necessary for the formation of a functional protein essential for the virion maturation 46 …”

“…44 One of these amino acids is L-arginine, essential for the replication of some viruses and the progression of infections, 45 raising the hypothesis that it would be necessary for the formation of a functional protein essential for the virion maturation. 46 Certain viruses remain in their latent form (inactive) and this characteristic is explained by the unavailability of arginine during stages of the infectious process. In the absence of arginine, the viral DNA synthesis is impaired, and the formation of virions is inhibited.…”

L‐lysine: Its antagonism with L‐arginine in controlling viral infection. Narrative literature review

“…26 For successful viral multiplication control, treatment with L-lysine must be instituted at the beginning of the manifestations, in the prodromal phase, with loading doses in order to compete with arginine, an important amino acid for initial viral replication. 23,25,26,97,99 The same is recommended with the use of allopathic antivirals. 61 This protocol prevents many lesions from progressing to the clinical phase, disappearing even in the prodromal phase or at the first clinical symptoms.…”

The Nature of the DNA Associated with Incomplete Particles of Adenovirus Type 2

“…Adenovirus IP have been under scrutiny as possible assembly intermediates. Several types of evidence support IP as precursors to mature virions: (i) pulse-chase experiments show that IP are synthesized before mature virions (Rosenwirth et al, 1974;Khittoo & Weber, 1977;Sundquist et al, 1973); (ii) IP contain precursor proteins (Ishibashi & Maizel, 1974;Khittoo & Weber, 1977); (iii) at least two types of particles exist, completely empty or containing DNA fragments, which may be possible intermediates during insertion of G. KHITTOO AND J. M. WEBER DNA (Edvardsson et al, 1976;Everitt et al, 1977;Tibbetts, 1977); (iv) H2ts4, H2tsll2, H2tsl01 and H5ts58 accumulate IP similar to those of wild-type (wt)-IP and block virus maturation (Khittoo & Weber, 1977;D'Halluin et al, 1978;Edvardsson et al, 1978;Boudin et al, 1980); (v) the production of IP is sensitive to arginine deprivation only prior to 20 h post-infection, while the assembly of complete virions is dependent on the continuous presence of arginine (Plaat & Weber, 1979).…”