On the Mechanism of the Lymphocytosis Following Pertussis Vaccination

Fichtelius, Karl-Erik; Gisslén, H; Hassler, Ove

doi:10.1159/000205224

Cited by 22 publications

(8 citation statements)

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“…It is not greater after than before birth [58], in conventional animals than in germ-free ones [80,88], and it seems to remain unchanged after antigenic stimulation [57,58]. The exception to these findings is a lipopolysaccharide antigen, the per-tussis vaccine, which can cause a change in thymus cell proliferation [20,21,66]. The results of the present study and the earlier quoted study of thymus lymphoid cell export in normal guinea pigs [48] show that stimulation of cell-mediated immunity leads to a change in thymus cell migration pattern in favor of the sensitized lymph node.…”

Section: Discussionmentioning

confidence: 99%

Influence of Contact Allergy on Thymus Lymphoid Cell Migration

Linna¹

1970

Int Arch Allergy Immunol

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Section: Discussionmentioning

confidence: 99%

Influence of Contact Allergy on Thymus Lymphoid Cell Migration

Linna¹

1970

Int Arch Allergy Immunol

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“…The rapid cell turnover of the thymus has been known for a long time. It has been established from studies of mitotic activity (Kindred 1940, Andreasen & Christensen 1949, Osogoe et al 1957, St. Marie & Leblond 1958a and from studies of the uptake of radioactive precursors into nucleic acids (Andreasen & Ottesen 1945, Fichtelius et al 1957, Schooley et al 1959, Mitchell et al 1963, Craddock et al 1964.…”

Section: Groupmentioning

confidence: 99%

Pattern of Cell Production and Cell Migration in Mouse Thymus Studied by Autoradiography

Borum

1968

Scandinavian Journal of Haematology

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Autoradiographs prepared from histological sections of thymus were studied at different intervals after a single intraperitoneal injection of tritiated thymidine into male CBA mice. The initial uptake was located mainly in the cortex, especially in the periphery, to a lesser extent in the medulla. Immediate incorporation was seen in reticular cells and in large and medium‐sized lymphocytes, not in small lymphocytes. As time elapsed more cells became labelled, and a migration of labelled cells from the cortex to the medulla took place. Simultaneously an increasing number of small thymocytes acquired the label. The pattern of initial uptake and spread of label with time was similar for the thymus of mice aged 3 weeks, 7 weeks and one year. Autoradiographs of smears of whole thymus revealed that 13 per cent of the cells were labelled one hour after the administration of 3H‐thymidine. The labelling percentage increased with time and reached a maximum of 37 per cent, 3 days after the injection. After that time a fall in labelling percentage was observed with a final disappearance of labelled cells from the thymus about the 14th day. Calculations on the basis of mitotic counts with and without colchicine pre‐treatment gave an average mitotic rate in thymic cortex of 3.7 per cent per hour, which renders an average renewal time of 27 hours by cell division for thymic cortex ‐ indepent of age. It is concluded that a large cell production takes place in the thymus, especially in the outer cortex, the end result being the production of thymocytes, mainly small ones, which migrate to the medulla from where they disappear.

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“…Although chicken bursa cells synthesize immunoglobulins [21, 221, it has so far not been shown that they have the capacity t o form specific antibodies [23, 241. According to the current view, cell proliferation in the central lymphoid organs is not changed by antigenic stimulation [25]. The exceptions t o these findings are thymus cell proliferation after stimulation with pertussis vaccine [26,27], and with pig serum in hypophysectomized rats [ 281.…”

Section: Introductionmentioning

confidence: 99%

Influence of antigenic stimulation on lymphoid cell traffic in the chicken

Bäck

Linna

1973

Eur J Immunol

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These individual probabilities are given by the hypergeometric distribution and the result reduces to:where C is independent of N, and bl+bol+bool+bml = total number (D) of different antibody molecules detected in the whole sample.From this result, relative probabilities for different values of N can be calculated using Stirling's approximation for factorial For hand calculation, the value of N giving the greatest probability (i.e. the maximum likelihood estimate of N) can most easily be found by considering the ratio of these probabilities for successive values of N. The estimate is then the largest valur of N for which Probabilities corresponding t o the data of Table 4 (i.e. n l =138, n,=6l, n3=67, n4=71, D=332) are shown in Fig. 4. If all assumptions are correct, a value of approximately 8000 can be given as an estimate of N, although clearly there is even then a wide range of likely values.Within a particular donor/recipients system some antibody types may have a greater chance of being sampled than others. However assumption (ii) is invalid only if this difference depends on some property of the antibody types (i.e. if some types have a relatively high probability of selection in all donor/recipients systems). If assumption (ii) is invalid, or if some of the spectra that were considered identical were in fact produced by different antibodies, the above method might be expected t o underestimate N. We might then consider 2000 to represent the smallest likely value, but no upper limit could then be given.However, let us now consider the case in which each mouse is genetically capable of producing only A (less than N) different antibody types (i.e. assumption (i) is invalid), and the A different types are not necessarily the same for all four mice. If we accept assumption (ii), the method given above should in this case be regarded as providing an overestimate of A rather than an estimate of N. If A is only slightly less than N, the degree by which A is overestimated should be small and 2000 as a minimum estimate of both A and N is unlikely t o be misleading. If A is much less than N, then, in general, no meaning can be attached t o the estimate. However, in this particular case, estimates of the number of different antibodies actually produced by a donor mouse (based on an analysis of the frequency of occurrence of identical antibodies in different mice receiving cell transfer from the same donor mouse) suggest that 2000, as a minimum estimate of N, is still unlikely t o be misleading.

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On the Mechanism of the Lymphocytosis Following Pertussis Vaccination

Cited by 22 publications

References 0 publications

Influence of Contact Allergy on Thymus Lymphoid Cell Migration

Influence of Contact Allergy on Thymus Lymphoid Cell Migration

Pattern of Cell Production and Cell Migration in Mouse Thymus Studied by Autoradiography

Influence of antigenic stimulation on lymphoid cell traffic in the chicken

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