On the morphology of hog cholera virus

Horzinek, Marian C.; Reczko, E; Petzoldt, K

doi:10.1007/bf01241748

Cited by 19 publications

(10 citation statements)

References 4 publications

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“…CSFV samples prepared by both methods were biologically active and capable of infecting PK-15 cells, but TFF-SEC method removed much more contaminating proteins inform the culture medium than ultracentrifugation. Electron microscopy revealed the presence of mature CSFV particles with a diameter of 40-60 nm in TFF-SEC-purified samples, and the characteristic morphology and size of purified CSFV viral particles in this study matched those described previously [24] . The antibody levels from immunization with CSFV prepared by the two methods showed significant difference (P < 0.05), and the existence of contaminants in ultracentrifugationprepared CSFV may reduce the efficiency of the animal's immune response.…”

Section: Discussionsupporting

confidence: 89%

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Wang¹,

Zhi²,

Guo³

et al. 2015

Front. Agr. Sci. Eng.

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Large-scale production of cell culture-based classical swine fever virus (CSFV) vaccine is hampered by the adverse reactions caused by contaminants from host cell and culture medium. Hence, we have developed an efficient method for purifying CSFV from cell-culture medium. Pure viral particles were obtained with two steps of tangential-flow filtration (TFF) and size-exclusion chromatography (SEC), and were compared with particles from ultracentrifugation by transmission electron microscopy (TEM), infectivity and recovery test, and real time fluorescent quantitative PCR (FQ-PCR). TFF concentrated the virus particles effectively with a retention rate of 98.5%, and 86.2% of viral particles were obtained from the ultrafiltration retentate through a Sepharose 4 F F column on a biological liquid chromatography system. CSFV purified by TFF-SEC or ultracentrifugation were both biologically active from 1.0Â10 -4.25 TCID 50 $mL -1 to 3.0Â10 -6.25 TCID 50 $mL -1 , but the combination of TFF and SEC produced more pure virus particles than by ultracentrifugation alone. In addition, pure CSFV particles with the expected diameter of 40-60 nm were roughly spherical without any visible contamination. Mice immunized with CSFV purified by TFF-SEC produced higher antibody levels compared with immunization with ultracentrifugation-purified CSFV (P < 0.05). The purification procedures in this study are reliable technically and feasible for purification of large volumes of viruses.

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Section: Discussionsupporting

confidence: 89%

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Wang¹,

Zhi²,

Guo³

et al. 2015

Front. Agr. Sci. Eng.

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“…Pestiviruses are exceedingly difficult to purify from cellular debris, and the demonstration of intact virus particles has so far been successful only for pestivirus isolates growing to high virus titers in tissue culture. Even for those, no high-resolution electron micrographs have been put forward that go beyond the raw description of enveloped round particles of 40 to 60 nm in diameter with putative icosahedral symmetry (13,14,24,34). With the complications of lower titers and genetic instabilities, we failed to obtain meaningful electron micrographs of CC or CY X C viruses.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Essential Domains and Plasticity of the Classical Swine Fever Virus Core Protein

Riedel

Lamp

Heimann

et al. 2010

J Virol

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genes between two Core genes. Even a Core fusion protein with more than 10-fold-increased molecular mass was integrated into the viral particle and supported the production of infectious progeny virus. The unexpected plasticity of Core protein brings into question the formation of a regular icosahedric particle and supports the idea of a histone-like protein-RNA interaction. All viruses with a duplicated Core gene were unstable and reverted to the wild-type sequence. Interestingly, a nonviable YFP-Core construct was rescued by a mutation within the C-terminal domain of the nonstructural protein NS3.

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“…However, in our study, podocytes were affected with conspicuous swelling in which vesicles contained numerous electron dense spheres corresponding to viral particles. Previous studies undertaken specifically to document the CSF viral particles under transmission electron microscopy, reveal the viruses as spherical structures with an electron dense core and a less electron dense outer coat, with an average diameter of 50 nm [2,9,13,14].…”

Section: Discussionmentioning

confidence: 99%

Ultrastructural glomerular changes in experimental infection with the classical swine fever virus

2000

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-Ultrastructural studies of glomerular changes were performed on 16 pigs experimentally infected with a highly virulent strain of the classical swine fever virus. Our observations revealed the thickening of glomerular basement membranes, swelling of endothelial cells and cytoplasmic vacuolization within podocytes containing abundant viral particles. An early viral infection of podocytes was suggested as the cause of selective swelling of the foot processes of these cells with the consequent obliteration of the glomerular urinary spaces. To our knowledge this is the first report of ultrastructural evidence of classical swine fever virions infecting glomerular podocytes.classical swine fever / glomerulus / ultrastructure / pathology Résumé -Évolution de l'ultrastructure des glomérules rénaux lors d'une infection expéri-mentale par le virus de la peste porcine classique. Une étude en microscopie électronique des glomérules rénaux a été réalisée afin de mieux appréhender l'évolution de leur ultrastructure lors de l'atteinte rénale associée à la peste porcine classique. Les prélèvements ont été réalisés sur 16 porcelets infectés expérimentalement par une souche très virulente du virus de la fièvre porcine classique. Nos observations ont montré un épaississement de la membrane basale, une tuméfaction des cellules endothéliales associée à la présence de vacuoles dans le cytoplasme des podocytes. De nombreuses particules virales ont été observées dans le cytoplasme des podocytes. Une infection virale précoce des podocytes semble probable, et pourrait expliquer la tuméfaction spécifique de la protubérance podale de ces cellules, et par voie de conséquence, l'obstruction de l'espace urinaire glomérulaire. Actuellement, cette étude est la première à mettre en évidence par ultrastructure des particules virales de la peste porcine classique dans les podocytes glomérulaires. fièvre porcine classique / glomérule rénal / pathologie / ultrastructure Vet. Res. 31 (2000)

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On the morphology of hog cholera virus

Cited by 19 publications

References 4 publications

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Efficient purification of cell culture-derived classical swine fever virus by ultrafiltration and size-exclusion chromatography

Characterization of Essential Domains and Plasticity of the Classical Swine Fever Virus Core Protein

Ultrastructural glomerular changes in experimental infection with the classical swine fever virus

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