On the mycelial growth of the lorel or false morel, Gyromitra esculenta (Pers.) Fr

Abstract

On the mycelial growth of the lorel or false morel, Gyromitra esculenta (Pers.) Fr.ILMA ROPONEN and MATTI KREULA ROPONEN, I . A KREULA, M. 1978: On the mycelial growth of the torel or false morel, Gyromitra escultenta (Pers.) Fr. -Karstenia 18 (suppl.).The growth factors of lore I mycelia were investigated in solid and I iquid media. The mycelial strains used in the experiments were isolated by germinating spores of lore Is in Petri dishes on nutr i ant agar. The strains i so I a ted were stored in the co I d in test tubes as pure cultures, and propagated vegetatively by Inoculation. The results showed that the production of spawn is pass i b I e in axen 1 c substrates. This method of cuI t i vat ion needs a ·high degree of sanitation . Spawn growth is at a maxi mum after 3-4 weeks, when the substrate i s fully covered with the mycelium.The pH range 4-7 was observed to be fabourable for the growth of the myce l ia tested . The mycelia grew vigorously when formic acid was added to a concentration of 0.01-0.1 per cent in the substrate, but growth was inhibited when the concentratIon was 0. 2 per cen. or more. The effect of temperature on mycelial growth was tested at +5°, 10°, 15°, 20° and 30° C; optimum growth was obtained at 10-25° C. The mycelia were very sensitive to elev at=~b;~fa~~~centratlons, and effective aeration and shaking were needed for growth in 1 iquid Waste materials from the prepared pulp industry containing hemicel tutose and t ignin appear to be su itab le for the cultivation of lore I mycelia after the addition of a small amount of urea.