This study assessed the knowledge, attitude, and practices (KAP) of the population in three districts in Tanzania on issues related to mycotoxin contamination and exposure. The study employed a cross-sectional design. Data were collected using a questionnaire survey, which was administered to gather quantitative data in 180 randomly selected households in three districts of Tanzania. In addition, qualitative data were collected using key informant interviews (KIIs) of 12 purposively selected respondents and 6 focus group discussions (FGDs) of six to twelve participants. Quantitative data were analysed in SPSS version 20 for Windows 180 using descriptive statistics and a chi-square test. While the qualitative data were analysed in ATLAS.ti 8 for Windows. A majority of respondents (25.1%) had never heard of mycotoxins, 20.1% were not aware of how mycotoxins are acquired, and none (0%) of the respondents claimed to never become sick after eating moulded/contaminated crops. The majority, 14.5% and 2.8% of the respondents, were not aware of prevention measures for animals and humans from mycotoxins, respectively. Only 8.9% agree that they are at risk of getting mycotoxins, 14% agree that it is safe to eat contaminated food, and 52.5% do not discard the mycotoxins contaminated food. The majority, 81.6%, dry their crops on top of the floor or bare grounds, almost 42.5% do not sort their crops before storage, and 28.5% agree to consume the defective/sorted crops. Therefore, it is recommended that health education interventions to create awareness among the public should be a priority and should be integrated into the existing control strategies