The study aimed to evaluate the tensile strength of additively manufactured (AMed) IN 625 using sub-sized test pieces and compare them to standard specimens. Cylindrical round coupons of varying diameters were manufactured along the Z-axis using the laser powder bed fusion technique and subjected to heat treatment. The simulation of the alloy solidification predicted the formation of several intermetallics and carbides under equilibrium conditions (slow cooling), apart from the γ phase (FCC). Sub-sized tensile specimens with different gauge diameters were machined from the coupons and tensile tested at ambient temperature. The results showed that sub-sized specimens exhibited lower tensile and yield strengths compared to standard specimens, but still higher than the minimum requirements of the relevant ASTM standard for AMed IN 625. The lower strength was attributed to the “size effect” of the test specimens. Fracture surfaces of the sub-sized test specimens exhibit a mixed character, combining cleavage and microvoid coalescence, with improved ductility compared to standard test pieces. The study highlights the importance of adapting characterization methods to the particularities of manufactured parts, including reduced thicknesses that make sampling standard-size specimens impractical. It concludes that sub-sized specimens are valuable for quality control and verifying compliance with requirements of AMed IN 625 tensile properties.