On the occurrence in vitro of cells resembling osteoclasts

Hancox, N. M.

doi:10.1113/jphysiol.1946.sp004153

Cited by 39 publications

(22 citation statements)

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“…The shape of the cells shows no orientation in relation to the growth pattern of the bone, such as might be expected if outward migration by active cell movement were occurring. Rumjantzew & Berezkina (1945) report that the osteoclasts are non-motile in tissue culture, though they also describe large osteoclasts migrating out of the original explant; more recently Hancox (1946) has described similar migration from chick frontal bone cultures. A rate of movement of the order of 10 an hour would suffice to keep pace with the growth of the bone.…”

Section: H I G H -P O W E R O B S E R V a T I O N S O N T H E O S T Ementioning

confidence: 99%

The supravital staining of osteoclasts with neutral-red: their distribution on the parietal bone of normal growing mice, and a comparison with the mutants grey-lethal and hydrocephalus-3

Barnicot

1947

Proc. R. Soc. Lond. B

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It is shown that the osteoclasts can be supravitally stained with neutral-red so that they are clearly visible under the dissecting microscope. Their distribution on the parietal bone of normal mice at several ages from birth to 28 days and the detailed appearance of neutral-red stained osteoclasts is described. It is shown that the staining reaction varies between individual cells, and some evidence is presented as to the relation of the neutral-red granules to cytoplasmic inclusions and to the Golgi apparatus. The bearing of the data on the origin and fate of osteoclasts is discussed. It is suggested that some of the small osteoclasts arise by separation from larger ones. Preliminary experiments on the application of the method to other parts of the skeleton by injection of the dye are described. The distribution of osteoclasts on the parietal bone of the grey-lethal and hydrocephalus-3 mutants is compared with the normal and the occurrence of giant osteoclasts in the grey-lethal is noted. A comparison of the normal and the grey-lethal by osteoclast counts is reported, and the accuracy of the method assessed. It is shown that the grey-lethal is deficient in number of osteoclasts, and possibly in the amount of osteoclast material. The distribution of osteoclast size in the normal and grey-lethal is compared. Some critical suggestions are put forward concerning the influence of mechanical factors in cranial growth, and the bearing of the present data on the problem is considered. Emphasis is laid on the tendency for a normal pattern of osteoclasts to be maintained even when the mechanical conditions are probably abnormal.

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Section: H I G H -P O W E R O B S E R V a T I O N S O N T H E O S T Ementioning

confidence: 99%

The supravital staining of osteoclasts with neutral-red: their distribution on the parietal bone of normal growing mice, and a comparison with the mutants grey-lethal and hydrocephalus-3

Barnicot

1947

Proc. R. Soc. Lond. B

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“…The giant multinucleate syncytium shown in Fig. 9 in the present paper resembles the giant cells in chick blood figured by Weiss and Fawcett (1952) and the giant cells around the periphery of outgrowth from small fragments of chick membrane bone (Hancox 1946). Such cells are probably all formed in the same manner, i.e.…”

Section: Discussionmentioning

confidence: 49%

A Karyological Study of Cultured Cells of <i>Limnodynastes peroni</i> (Anura: Leptodactylidae)

Robinson

Stephenson

1967

CYTOLOGIA

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“…An extremely active undulating membrane appeared at the periphery of the multi nucleated giant cells (figs. 4,5). Their movements suggested amoebocytes.…”

Section: Resultsmentioning

confidence: 99%

Growth and Maintenance of Human Giant-cell Bone Tumors (Osteoclastomas) in Continuous Culture

Gallardo¹,

Lustig²,

Schajowicz³

1970

Oncology

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Tissue culture of six human giant cell tumors of bone was undertaken (one on two occasions). All of them were cultivated successfully. Five cultures have shown outgrowth in about 25 days. In the remaining two cases continuous culture (nine generations) was successfully carried out. From this study the following results were obtained: the giant cells usually persist for about 48 to 72 h in primary tissue cultures. New giant cells were born during the following days in vitro. In trypsinized material the two cultures obtained lost their primitive growth features after the second generation. However, the ability to form giant cells is not definitely lost as is demonstrated by organ culture technique. The multinucleated giant cells exhibit, in primary tissue cultures, the same cytological and cytochemical features as normal osteoclasts and giant cell tumors. The mechanism of giant cell formation seems to be direct amitotic division, fusion of two or more mononuclear cells, or a combination of both. Furthermore, it was possible to detect a resting nucleus and another nucleus in mitotic division, side-by-side in the same cell, an unusual phenomenon in cell biology.

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On the occurrence in vitro of cells resembling osteoclasts

Cited by 39 publications

References 0 publications

The supravital staining of osteoclasts with neutral-red: their distribution on the parietal bone of normal growing mice, and a comparison with the mutants grey-lethal and hydrocephalus-3

The supravital staining of osteoclasts with neutral-red: their distribution on the parietal bone of normal growing mice, and a comparison with the mutants grey-lethal and hydrocephalus-3

A Karyological Study of Cultured Cells of <i>Limnodynastes peroni</i> (Anura: Leptodactylidae)

Growth and Maintenance of Human Giant-cell Bone Tumors (Osteoclastomas) in Continuous Culture

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