On the origin of electrochemical surface-enhanced Raman spectroscopy (EC-SERS) signals for bacterial samples: the importance of filtered control studies in the development of new bacterial screening platforms

McLeod, Kaleigh; Lynk, Taylor P.; Sit, Clarissa S.; Brosseau, Christa L.

doi:10.1039/c8ay02613k

Cited by 11 publications

(6 citation statements)

References 18 publications

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“…Gram-positive B. megaterium and Gram-negative E. coli K-12 were detected and differentiated with improved SERS peak intensities and spectral richness, showing a promise for bacterial screening. Further coupled the EC-SERS method with filter system, control studies were performed to ascertain the EC-SERS signals for bacterial sample [139]. It was reported that the SERS spectra collected for bacterial cultures were due to the released small molecules metabolites in response to environmental stressors.…”

Section: Discussionmentioning

confidence: 99%

In situ food-borne pathogen sensors in a nanoconfined space by surface enhanced Raman scattering

Ying

2021

Microchim Acta

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The incidence of disease arising from food-borne pathogens is increasing continuously and has become a global public health problem. Rapid and accurate identification of food-borne pathogens is essential for adopting disease intervention strategies and controlling the spread of epidemics. Surface-enhanced Raman spectroscopy (SERS) has attracted increasing interest due to the attractive features including simplicity, rapid measurement, and high sensitivity. It can be used for rapid in situ sensing of single and multicomponent samples within the nanostructure-based confined space by providing molecular fingerprint information and has been demonstrated to be an effective detection strategy for pathogens. This article aims to review the application of SERS to the rapid sensing of food-borne pathogens in food matrices. The mechanisms and advantages of SERS, and detection strategies are briefly discussed. The latest progress on the use of SERS for rapid detection of food-borne bacteria and viruses is considered, including both the labeled and label-free detection strategies. In closing, according to the current situation regarding detection of food-borne pathogens, the review highlights the challenges faced by SERS and the prospects for new applications in food safety.

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Section: Discussionmentioning

confidence: 99%

In situ food-borne pathogen sensors in a nanoconfined space by surface enhanced Raman scattering

Ying

2021

Microchim Acta

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“…EC-SERS is a technology that combines electrochemistry and SERS. Recently, many analyses have shown that it greatly improves the sensitivity of SERS [ 16 , 17 ]. Brindersi et al [ 18 ] showed that Fabric-based electrodes provide excellent EC-SERS signals, allowing rapid detection of levofloxacin at clinically relevant concentrations.…”

Section: Introductionmentioning

confidence: 99%

Integrated EC-SERS Chip with Uniform Nanostructured EC-SERS Active Working Electrode for Rapid Detection of Uric Acid

Huang

Hsiao

2020

Sensors

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Toxemia of pregnancy is a very dangerous disease for pregnant women. The mortality rate of toxemia of pregnancy is close to 10% to 15%. Early detection of pregnancy toxemia is to monitoring uric acid concentration in urine. The current mainstream method for detecting uric acid requires an enzyme (urate oxidase), which needs to be stored in a low-temperature environment, and the method requires complex chemical steps, which takes a longer time and more samples. In this study, we propose an integrated miniature three-electrode electrochemical surface-enhanced Raman spectroscopy chip (EC-SERS chip) suitable for rapid EC-SERS detection applications. The integrated microfluidic reservoir on the chip makes it easy to use, which is very suitable for rapid detection applications. The SERS active working electrode for the proposed integrated EC-SERS chip is a nanocone array polycarbonate (PC) substrate decorated with an evenly distributed and tightly packed array of gold nanospheres. It showed good uniformity and can be easily reproduced. The integrated EC-SERS chip is very small compared to the traditional electrochemical cell, which reduces the sample volume required for the testing. In addition, the chip is for one-time use only. It eliminates the need to clean electrochemical cells for reuse, thereby reducing the possibility of contamination and inaccurate detection. Various low-concentration Rhodamine 6G (R6G) solutions were tested to verify the performance of the developed EC-SERS chip. Experimental results showed that the proposed EC-SERS chip has a strong enhancement factor of up to 8.5 × 106 and a very good EC-SERS uniformity (the relative standard deviation of EC-SERS intensity is as low as 1.41%). The EC-SERS chip developed has been further tested for the detection of uric acid in synthetic urine. The results showed that the EC-SERS signal intensity has a highly linear relationship with the logarithm of the uric acid concentration in synthetic urine, which indicates that the developed EC-SERS chip is suitable for the quantitative detection of uric acid in synthetic urine. Therefore, the developed EC-SERS chip is very promising to be used in routine and early diagnosis of pregnancy toxemia and may be used in many other medical tests, food safety, and biotechnology applications.

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“…The benefits of the specific adsorption of target species via EC-SERS for improved signal generation have been shown in previous research. Nonetheless, aside from two reports , focusing on using a classical EC-SERS approach for the detection of Gr + and/or Gr – , involving a 16 h and a 6 h preincubation, respectively; essentially, in the case of mycobacteria, EC-SERS is an underexplored area and has not been reported yet. Moreover, with classical EC-SERS on preformed silver nanometallic surfaces, positive voltages are usually strictly avoided due to the potential for oxidation and its associated negative impact on the SERS activity of the nanoparticles. , However, in this study, the application of a positive voltage forms an integral part of the EC-SERS detection technique.…”

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confidence: 99%

Dual-Approach Electrochemical Surface-Enhanced Raman Scattering Detection of Mycobacterium tuberculosis in Patient-Derived Biological Specimens: Proof of Concept for a Generalizable Method to Detect and Identify Bacterial Pathogens

et al. 2022

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The recent surge in infectious disease-causing pathogens, resulting in global catastrophe, has merited a pivotal quest toward point-of-care (POC) diagnostics. Mycobacterium tuberculosis (MTB) is still the top bacterium-based infectious disease-causing pathogen worldwide. In a concerted effort toward simplifying and decentralizing the discriminatory screening of MTB causing pathogens, electrochemical surface-enhanced Raman scattering (EC-SERS) was adopted to create a customized screening tool. The development strategy combined five key factors, including (i) a simplified Tollens'-based chemical synthesis method for bulk supply of silver nanoparticles, (ii) the deliberate surface modification of nanoparticles with carefully selected polyelectrolytes to resemble the conditioning layer usually found on a natural substratum, (iii) uniform SERSactive films formed through simple unprogrammed assembly, (iv) the controlled manipulation of the local electric field through applied voltage using a technique that does not conform to the limitations of classical EC-SERS, and (v) the inherent specificity of the target-specific SERS vibrational signature. The EC-SERS platform was able to discriminatively detect and identify TB-derived mycobacteria, including three clinically relevant MTB strains, TB-H37Rv, TB-HN878, and TB-CDC1551. Moreover, a customized voltage stepping protocol, compatible with either the inclusion of a short preincubation step or with in situ EC-SERS is illustrated. From the obtained SERS vibrational signatures, a band indicating a mode unique to TB-derived/TB-affiliated mycobacteria and thus not observed for other bacterial types used in this study was illustrated. Furthermore, provisional investigation, done as prelude for assessing the potential for translational adaptability of the EC-SERS technique toward POC clinical settings for sputum and urine specimens, was carried out.

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On the origin of electrochemical surface-enhanced Raman spectroscopy (EC-SERS) signals for bacterial samples: the importance of filtered control studies in the development of new bacterial screening platforms

Abstract: The importance of filtered control studies for ascertaining the origin of bacterial SERS signals is highlighted.

Cited by 11 publications

References 18 publications

In situ food-borne pathogen sensors in a nanoconfined space by surface enhanced Raman scattering

In situ food-borne pathogen sensors in a nanoconfined space by surface enhanced Raman scattering

Integrated EC-SERS Chip with Uniform Nanostructured EC-SERS Active Working Electrode for Rapid Detection of Uric Acid

Dual-Approach Electrochemical Surface-Enhanced Raman Scattering Detection of Mycobacterium tuberculosis in Patient-Derived Biological Specimens: Proof of Concept for a Generalizable Method to Detect and Identify Bacterial Pathogens

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