On the Preparation of Highly Purified Slow Reacting Substance of Anaphylaxis (Srs‐a) From Biological Extracts

Blackwell, G.J.; Burka, John F.; Flower, Roderick J.; Torkington, P.

doi:10.1111/j.1476-5381.1980.tb10696.x

Cited by 6 publications

(4 citation statements)

References 33 publications

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“…SRS activity (either from platelets or peritoneal cells) was eluted only with ethanol/concentrated ammonia/water (6:3:1 vol/vol). Furthermore, platelet and peritoneal cell-derived SRS exhibited similar RP-HPLC elution patterns that were identical to those reported for guinea pig lung in (19) (Fig. 2).…”

Section: Methodssupporting

confidence: 77%

“…They could be due to storage in deep freeze, an effect that has already been observed (19). However, SRS appears to belong to several molecular species derived from AA (27)(28)(29)(30)(31)(32) (26,35,36).…”

Section: Methodsmentioning

confidence: 75%

“…using a reverse phase column (Bondapak C 18) (RP-HPLC). Elution was carried out with two types of methanol-water gradients as in (19,20 (Table I). In contrast, ADP (10 ,uM) caused platelet aggregation but there was no release of SRS activity above controls.…”

Section: Methodsmentioning

confidence: 99%

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Release of a slow-reacting substance from rabbit platelets.

Mencia‐Huerta¹,

Hadji²,

Benveniste³

1981

J. Clin. Invest.

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A B S T R A C T Washed rabbit platelets stimulated with platelet-activating factor, thrombin, or arachidonic acid, released a slow-reacting substance (SRS), whereas platelets aggregated by adenosine diphosphate did not. Production of platelet-derived SRS was neither affected by indomethacin nor aspirin but was reduced by large doses of eicosatetraynoic acid, an inhibitor of the cyclooxygenase and lipoxygenase. L-cysteine enhanced markedly the release of SRS from platelets. This SRS activity, which was antagonized by FPL 55712 and inactivated by arylsulfatase, followed the same elution pattern on Amberlite, silicic acid, and reverse phase high-pressure liquid chromatography columns as that described for the SRS from other origins. SRS activity released from platelets preincubated with [14C]arachidonic acid exhibited the same retention time as radioactivity in reverse phase high-pressure liquid chromatography. The release of a SRS from platelets is consistent with their implication in the pathogenesis of asthma and other lung diseases.

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Section: Methodssupporting

confidence: 77%

Section: Methodsmentioning

confidence: 75%

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Release of a slow-reacting substance from rabbit platelets.

Mencia‐Huerta¹,

Hadji²,

Benveniste³

1981

J. Clin. Invest.

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“…These workers have postulated on this evidence that ionophore-induced SRS and possibly immunologicallyinduced SRS-A are metabolites of arachidonic acid. Recent studies in our laboratory have indicated that the slow-reacting substances induced by either stimulus are chromatographically indistinguishable (Blackwell, Burka & Flower, 1978). Therefore, we shall use the term SRS-A so that our results can relate to the classical substance described by Brocklehurst (1960).…”

Section: Introductionmentioning

confidence: 99%

Effects of Modulators of Arachidonic Acid Metabolism on the Synthesis and Release of Slow‐reacting Substance of Anaphylaxis

Burka

Flower

1979

British J Pharmacology

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Slow‐reacting substance of anaphylaxis (SRS‐A) was released in the peritoneum of passively sensitized rats challenged with ovalbumin and from rat isolated peritoneal cells stimulated with calcium ionophore A23187. Both monocytes (macrophages) and mast cells appear to be involved in the synthesis and release of SRS‐A. The immunological release of SRS‐A in vivo is enhanced by indomethacin and inhibited by dexamethasone, mepacrine, 1‐phenyl‐3‐pyrazolidone (1‐P‐3‐P), and methylimidazole. SRS‐A release induced by A23187 in vitro is inhibited by dexamethasone, indomethacin, 1‐P‐3‐P, eicosatetraynoic acid (ETA) and 15‐hydroperoxy arachidonic acid. The inhibition induced by dexamethasone, indomethacin and 1‐P‐3‐P is reduced by an increase in the calcium concentration from 1 mm to 5 mm, whereas the inhibition induced by ETA is increased. The results suggest that a lipoxygenase is important in the synthesis and release by SRS‐A.

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