On the relationship between respiratory activity and lipid composition of the yeast cell

Kováč, Ladislav; Šubík, Július; Russ, Gerald A.; Kollár, K.

doi:10.1016/0005-2760(67)90080-x

Cited by 45 publications

(2 citation statements)

References 39 publications

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“…The present data are in many ways quite similar to those previously reported for whole anaerobic yeast cells (Kováó et at., 1967;Jollow et al, 1968;Serlupi-Crescenzi and Barcellona, 1966). However, analyses of intact cells do not yield definitive information about the composition of the mitochondrial membranes and may also be ambiguous from a quantitative point of view.…”

Section: Discussionsupporting

confidence: 92%

Promitochondria of anaerobically grown yeast. II. Lipid composition

Paltauf¹,

Schatz²

1969

Biochemistry

111

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Section: Discussionsupporting

confidence: 92%

Promitochondria of anaerobically grown yeast. II. Lipid composition

Paltauf¹,

Schatz²

1969

Biochemistry

111

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“…In particular, the overall increase in GP unsaturation is largely due to the increased unsaturation of PA, PS and PE. The increase in unsaturation of PA and PS might lead to a sufficiently unsaturated pool of precursors for PE synthesis in the mitochondria by decarboxylation of PS [42], [43]. The idea that PS serves as a source for unsaturated PE is supported by the overall reduction of PS in yeast cells grown in non-glucose carbon sources.…”

Section: Discussionmentioning

confidence: 99%

Flexibility of a Eukaryotic Lipidome – Insights from Yeast Lipidomics

et al. 2012

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Mass spectrometry-based shotgun lipidomics has enabled the quantitative and comprehensive assessment of cellular lipid compositions. The yeast Saccharomyces cerevisiae has proven to be a particularly valuable experimental system for studying lipid-related cellular processes. Here, by applying our shotgun lipidomics platform, we investigated the influence of a variety of commonly used growth conditions on the yeast lipidome, including glycerophospholipids, triglycerides, ergosterol as well as complex sphingolipids. This extensive dataset allowed for a quantitative description of the intrinsic flexibility of a eukaryotic lipidome, thereby providing new insights into the adjustments of lipid biosynthetic pathways. In addition, we established a baseline for future lipidomic experiments in yeast. Finally, flexibility of lipidomic features is proposed as a new parameter for the description of the physiological state of an organism.

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Quantitative studies of the development of S. cerevisiae mitochondria

Ferdouse

Rickard

Moss

et al. 1972

Biotech & Bioengineering

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The quantitative changes in mitochondria and cytochromes during transition of Saccharomyces cerevisiae from one steady state to another, while growing in continuous culture under controlled environmental conditions, were followed. No Mitochondria, or mitochondria like structures, were detectable in electron micrographs of permanganate‐fixed anaerobic cells. Microaerobiosis (3μM dissolved oxygen) was sufficient to visualize mitochondrial profiles and induce cytochromes and their sections had a reduced number of mitochondrial profiles compared with cells grown in limiting glucose. In the presence of ergosterol and Tween 80 mitochondriogenesis, whether induced by aerobiosis or glucose limitation, involved enhanced definition of crystal and outer mitochondrial membranes and increased number of profiles. Where membrane formation was limited, by the absence of aerobiosis involved eytochrome induction and profile visualization, but limited profile Proliferation; the adapted cells consequently contained fewer, but more eytochrome‐enriched, mitochondria than cells adapted in the presence of ergosterol and Tween 80. Increase in dissolved oxygen from 3μM to 52μM further enhanced membrane definition and increased the size, but not the number, of mitochondrial profiles. Evidence, obtained by measurement of eytochrome concentration per unit mitochondrial volume and per unit crystal area, support the concept that mitochondriogensis and cytochrome synthesis are not synchronized process and that cytochromes are added to or depleted from the mitochondrial cristae in response to culture conditions.

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On the relationship between respiratory activity and lipid composition of the yeast cell

Cited by 45 publications

References 39 publications

Promitochondria of anaerobically grown yeast. II. Lipid composition

Promitochondria of anaerobically grown yeast. II. Lipid composition

Flexibility of a Eukaryotic Lipidome – Insights from Yeast Lipidomics

Quantitative studies of the development of S. cerevisiae mitochondria

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