On the relative efficiency of two‐ vs. one‐stage production of astaxanthin by the green alga <i>Haematococcus pluvialis</i>

Aflalo, Claude; Meshulam, Yuval; Zarka, Aliza; Boussiba, Sammy

doi:10.1002/bit.21391

Cited by 196 publications

(136 citation statements)

References 35 publications

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“…Meanwhile, the attached cultivation of microalgae to produce biofuel could be operated in a continuous mode, which means that a large amount of TAG could be produced directly in one-step continuous mode without increasing the light intensity or changing the composition of the medium. For the suspended cultivation, continuous production of secondary metabolites from microalgae is generally very low in efficiency and is limited to the production of high value bioactives (Aflalo et al 2007;García-Malea et al 2009). In case of producing cheap metabolites in large scale (e.g., TAG for biofuel), the batch mode (two-step) is the prevailing strategy in which the microalgae cells are first cultivated in unstressed conditions to accumulate biomass and then transferred to adverse conditions to induce the synthesis and accumulation of the target metabolites (Heieh and Wu 2009;Ratha et al 2013).…”

Section: Discussionmentioning

confidence: 99%

An applicable nitrogen supply strategy for attached cultivation of Aucutodesmus obliquus

Wang

Zhang

et al. 2013

J Appl Phycol

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The "attached cultivation" method of microalgae in which the wet paste of algal biomass is attached onto supporting materials to form an immobilized biofilm layer, and the culture medium is supplied to this layer to provide nutrients and moisture for growth was highly efficient in biomass production and represents a promising technology to improve the biofuel industry. To optimize the nitrogen supply strategy for this attached cultivation method, the growth and total lipids accumulation properties for the green alga Aucutodesmus obliquus with this method were studied under different quantities of nitrogen source and different volumes of aqueous medium that continuously circulated inside the photobioreactor. Results showed that, compared with medium volume, the nitrogen quantity was a stronger factor affecting the growth and total lipid accumulation. An optimized nitrogen supply strategy for the attached cultivation of A. obliquus is proposed as circulating ca. 60 L of BG-11 medium containing 1/10 of nitrate concentration for 1 m 2 of cultivation surface. With this strategy, the attached A. obliquus accumulated biomass and total lipids simultaneously and obtained a high triacylglyceride productivity of 2.53 g m Dissolving the nitrogen source in small volume was the best way to efficiently utilize the nitrogen source with minimum of waste.

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Section: Discussionmentioning

confidence: 99%

An applicable nitrogen supply strategy for attached cultivation of Aucutodesmus obliquus

Wang

Zhang

et al. 2013

J Appl Phycol

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“…The combination of nitrogen deprivation and HL exposure was used to facilitate stress response (10). Cells were harvested every 6 or 12 h throughout the 48 or 96 h of exposure to stress.…”

Section: Changes In Metabolic Status Caused By Nitrogen Starvation Unmentioning

confidence: 99%

“…Different stress conditions, including high salinity, high irradiance, and nutrient deprivation, induce the accumulation of astaxanthin to up to 4% of the cell's dry biomass (8,9). However, the highest rate of accumulation was observed under the combined stress of nitrogen starvation and high light (HL) 3 exposure (10). This accumulation is accompanied by massive morphological and metabolic changes (11), (e.g.…”

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confidence: 99%

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Metabolite Profiling and Integrative Modeling Reveal Metabolic Constraints for Carbon Partitioning under Nitrogen Starvation in the Green Algae Haematococcus pluvialis

Recht

Töpfer

Batushansky

et al. 2014

Journal of Biological Chemistry

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109

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Background:The microalga H. pluvialis shows a distinct pattern of carbon repartitioning upon nitrogen starvation. Results: Data-driven integrative modeling pinpoints metabolic adjustments underlying carbon repartition. Conclusion:In vitro and in silico experiments can dissect the systemic response to nitrogen starvation. Significance: Experimental data in conjunction with integrative modeling enables model-driven hypothesis testing.

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“…As such, a two‐stage cultivation mode is widely adopted for H. pluvialis mass culture, that is, producing green biomass under favorable conditions and inducing astaxanthin accumulation under stress (Aflalo et al, 2007; Fábregas et al, 2001). The limiting step of this culture mode is the supply of green biomass for astaxanthin production.…”

Section: Introductionmentioning

confidence: 99%

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

Zhang

Wang

et al. 2016

Biotech & Bioengineering

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The unicellular green alga Haematococcus pluvialis has been exploited as a cell factory to produce the high‐value antioxidant astaxanthin for over two decades, due to its superior ability to synthesize astaxanthin under adverse culture conditions. However, slow vegetative growth under favorable culture conditions and cell deterioration or death under stress conditions (e.g., high light, nitrogen starvation) has limited the astaxanthin production. In this study, a new paradigm that integrated heterotrophic cultivation, acclimation of heterotrophically grown cells to specific light/nutrient regimes, followed by induction of astaxanthin accumulation under photoautotrophic conditions was developed. First, the environmental conditions such as pH, carbon source, nitrogen regime, and light intensity, were optimized to induce astaxanthin accumulation in the dark‐grown cells. Although moderate astaxanthin content (e.g., 1% of dry weight) and astaxanthin productivity (2.5 mg L−1 day−1) were obtained under the optimized conditions, a considerable number of cells died off when subjected to stress for astaxanthin induction. To minimize the susceptibility of dark‐grown cells to light stress, the algal cells were acclimated, prior to light induction of astaxanthin biosynthesis, under moderate illumination in the presence of nitrogen. Introduction of this strategy significantly reduced the cell mortality rate under high‐light and resulted in increased cellular astaxanthin content and astaxanthin productivity. The productivity of astaxanthin was further improved to 10.5 mg L−1 day−1 by implementation of such a strategy in a bubbling column photobioreactor. Biochemical and physiological analyses suggested that rebuilding of photosynthetic apparatus including D1 protein and PsbO, and recovery of PSII activities, are essential for acclimation of dark‐grown cells under photo‐induction conditions. Biotechnol. Bioeng. 2016;113: 2088–2099. © 2016 The Authors. Biotechnology and Bioengineering Published by Wiley Periodicals, Inc.

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On the relative efficiency of two‐ vs. one‐stage production of astaxanthin by the green alga Haematococcus pluvialis

Cited by 196 publications

References 35 publications

An applicable nitrogen supply strategy for attached cultivation of Aucutodesmus obliquus

An applicable nitrogen supply strategy for attached cultivation of Aucutodesmus obliquus

Metabolite Profiling and Integrative Modeling Reveal Metabolic Constraints for Carbon Partitioning under Nitrogen Starvation in the Green Algae Haematococcus pluvialis

A new paradigm for producing astaxanthin from the unicellular green alga Haematococcus pluvialis

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