On the role of anisotropy of membrane constituents in formation of a membrane neck during budding of a multicomponent membrane

Iglič, Aleš; Babnik, Blaž; Bohinc, Klemen; Fošnarič, Miha; Hägerstrand, Henry; Kralj‐Iglič, Veronika

doi:10.1016/j.jbiomech.2006.02.006

Cited by 41 publications

(57 citation statements)

References 39 publications

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“…The ions, soluble molecules and macromolecules (e.g. proteins, carbohydrates, lipids) in the solution not only enter into chemical reactions with the membrane-bound receptors, but may affect the budding and vesiculation process via different physical mechanisms [13,[35][36][37][38][39][40]. The majority of MVs isolated from the peripheral blood derive from the platelet membrane [41]; however, assuming that the essential physical properties of all biological membranes are the same [42], the relevant mechanisms can be studied in simpler systems, such as erythrocytes and giant phospholipid vesicles (GPVs), which can be observed live under an optical microscope.…”

Section: Microvesiculationmentioning

confidence: 99%

“…The intrinsic shape of a flexible membrane nanodomain can be described by two different intrinsic principal curvatures, C 1m and C 2m [9,13,15,20,64]. If C 1m = C 2m , the nanodomain is isotropic, while if C 1m ≠ C 2m , the nanodomain is anisotropic.…”

Section: Curvature-mediated Lateral Redistribution Of Membrane Constimentioning

confidence: 99%

“…Taking into account the definition of the tensor M , it follows from Eq. (3) that the elastic energy of the single membrane constituent can be written as [13,20,45]: …”

Section: Curvature-mediated Lateral Redistribution Of Membrane Constimentioning

confidence: 99%

“…As the neck becomes narrower, the distribution approaches a step function, while the free energy of the nanodomains lowers the free energy of the membrane. The proposed mechanism of coupling of the local membrane shape and the lateral density of the membrane constituents (nanodomains) may therefore be one of the mechanisms which is relevant for the formation and stabilization of highly curved spherical membrane shapes (such as membrane buds) with increased concentrations of particular membrane constituents [13,15,45,82]. In considering the process of nanotube growth, it is assumed that the growing actin filaments push the membrane outwards.…”

Section: Curvature-mediated Lateral Redistribution Of Membrane Constimentioning

confidence: 99%

“…The nanotubes and microvesicles are small enough to be classified as nanostructures, with their radius ranging from fifty to a few hundred nm. An important feature of the formation mechanisms for both is that they are preceeded by lateral reorganization of the membrane into rafts and nanodomains [10][11][12][13][14][15]. As this process is mediated by the local curvature of the membrane, which is in turn determined by the local composition of the constituents, it is the intrinsic shape of the constituents and their interactions with the neighboring molecules [9,11,[15][16][17][18][19][20] that determine whether microvesiculation or tubulation of the membrane occurs [9,10,[13][14][15].…”

Section: Introductionmentioning

confidence: 99%

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Mechanisms for the formation of membranous nanostructures in cell-to-cell communication

Schara¹,

Janša

Šuštar

et al. 2009

Cellular and Molecular Biology Letters

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Cells interact by exchanging material and information. Two methods of cell-to-cell communication are by means of microvesicles and by means of nanotubes. Both microvesicles and nanotubes derive from the cell membrane and are able to transport the contents of the inner solution. In this review, we describe two physical mechanisms involved in the formation of microvesicles and nanotubes: curvature-mediated lateral redistribution of membrane components with the formation of membrane nanodomains; and plasmamediated attractive forces between membranes. These mechanisms are clinically relevant since they can be affected by drugs. In particular, the underlying mechanism of heparin's role as an anticoagulant and tumor suppressor is the suppression of microvesicluation due to plasma-mediated attractive interaction between membranes.

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Section: Microvesiculationmentioning

confidence: 99%

Section: Curvature-mediated Lateral Redistribution Of Membrane Constimentioning

confidence: 99%

“…Taking into account the definition of the tensor M , it follows from Eq. (3) that the elastic energy of the single membrane constituent can be written as [13,20,45]: …”

Section: Curvature-mediated Lateral Redistribution Of Membrane Constimentioning

confidence: 99%

Section: Curvature-mediated Lateral Redistribution Of Membrane Constimentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Mechanisms for the formation of membranous nanostructures in cell-to-cell communication

Schara¹,

Janša

Šuštar

et al. 2009

Cellular and Molecular Biology Letters

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Assembling of Topological Defects at Neck‐Shaped Membrane Parts

Kurioz

Mesarec

Iglič

et al. 2019

Physica Status Solidi (a)

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The impact of intrinsic and extrinsic curvature on the distribution of topological defects (TDs) in neck‐like regions of biological membranes is studied quantitatively. Biological membranes are modeled effectively at the mesoscopic level as two‐dimensional films described in terms of the tensor nematic order parameter field and curvature fields. It is demonstrated that antidefects robustly form at the neck area and can promote a membrane fission. The assembling of antidefects near the catenoid's equatorial ring, where catenoids roughly mimic neck shapes are analyzed in more detail. It is demonstrated that for sufficiently strong curvatures, the effective topological charge Δmeff within a strongly curved region equals zero, and the resulting structures are topologically neutral. Consequently, the total charge of antidefects within the region equals Δm = −ΔmV − ΔmK. In most cases, the positions of antidefects are strongly influenced by the extrinsic curvature.

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Attachment of β2-glycoprotein I to negatively charged liposomes may prevent the release of daughter vesicles from the parent membrane

et al. 2008

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The temperature-induced budding of POPC-cardiolipin-cholesterol, POPC-POPS-cholesterol and POPC-POPG-cholesterol giant lipid vesicles in the presence of beta 2-glycoprotein I (beta 2-GPI) in the outer solution was studied experimentally and theoretically. The observed budding transition of vesicles was continuous which can be explained by taking into account the orientational ordering and direct interactions between oriented lipids. The attachment of positively charged beta 2-GPI to the negatively charged outer surface of POPC-cardiolipin-cholesterol, POPC-POPS-cholesterol and POPC-POPG-cholesterol giant vesicles caused coalescence of the spheroidal membrane bud with the parent vesicle before the bud could detach from the parent vesicle, i.e. vesiculate. Theoretically, the protein-mediated attraction between the membrane of a bud and the parent membrane was described as an interaction between two electric double layers. It was shown that the specific spatial distribution of charge within beta 2-GPI molecules attached to the negatively charged membrane surface may explain the observed attraction between like-charged membrane surfaces.

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On the role of anisotropy of membrane constituents in formation of a membrane neck during budding of a multicomponent membrane

Cited by 41 publications

References 39 publications

Mechanisms for the formation of membranous nanostructures in cell-to-cell communication

Mechanisms for the formation of membranous nanostructures in cell-to-cell communication

Assembling of Topological Defects at Neck‐Shaped Membrane Parts

Attachment of β2-glycoprotein I to negatively charged liposomes may prevent the release of daughter vesicles from the parent membrane

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