On the role of serotonin and 5-methoxy-tryptamine in the regulation of cell division in sea urchin eggs

Renaud, Fernando L.; Parisi, Elio; Capasso, Antonio; Prisco, P. De

doi:10.1016/0012-1606(83)90333-0

Cited by 69 publications

(35 citation statements)

References 20 publications

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“…Based upon the previous pharmacological studies, 'prenervous' serotonin is involved in early cleavage of sea urchin development (Renaud et al, 1983;Shmukler, 1993;Shmukler and Tosti, 2001), and serotonin receptors have been suggested to be in the plasma membrane of blastomeres during cleavage period (Shmukler, 1993;Shmukler and Tosti, 2001). Serotonin is involved in ciliary beating regulation in embryos such as gastrulae (Soliman, 1983).…”

Section: Discussionmentioning

confidence: 99%

Serotonin stimulates [Ca2+]i elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel

Katow

Yaguchi

Kyozuka

2007

Journal of Experimental Biology

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SUMMARY A full-length serotonin receptor mRNA from the 5Hthpr gene was sequenced from larvae of the sea urchin, Hemicentrotus pulcherrimus.The DNA sequence was most similar to 5HT-1A of the sea urchin Strongylocentrotus purpuratus found by The Sea Urchin Genome Project,and the protein sequence predicted the presence of seven transmembrane domains. Immunohistochemistry with anti-5HThpr antibodies indicated that the protein was expressed on blastocoelar cells that comprised the major blastocoelar network (serotonin receptor cell network). These network cells inserted their processes into the ectoderm in various regions, including the ciliary band region. Serotonin injected into the blastocoel stimulated a transient elevation of cytoplasmic Ca2+ concentration([Ca2+]i) in the ectoderm, as detected by Oregon-Green dextran, injected earlier in development. The calcium transient propagated as a wave at about 175 μm s–1, but was not detectable in the serotonin receptor-positive cell network. In larvae treated with p-chlorophenylalanine, a potent and irreversible serotonin synthesis inhibitor, serotonin application did not stimulate[Ca2+]i, the serotonin receptor cell network did not develop properly, and the swimming behavior of the larvae was abnormal. However, formation of a different nervous system comprising synaptotagmin-possessed neurites was not affected by p-chlorophenylalanine treatment. These results imply that serotonin secreted from the apical ganglion into the blastocoel stimulates the elevation of [Ca2+]i in the larval ectodermal cells through the serotonin receptor cell network.

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Section: Discussionmentioning

confidence: 99%

Serotonin stimulates [Ca2+]i elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel

Katow

Yaguchi

Kyozuka

2007

Journal of Experimental Biology

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“…The most detailed results have been obtained for pre-nervous 5-HT in sea urchins [Buznikov, 1967;Buznikov et al, 2001Buznikov et al, , 1996Renaud et al, 1983;Toneby, 1977]. In particular, it has been shown that the concentration of 5-HT (or 5-HT-like substances) changes in developing sea urchin embryos and larvae, starting from the fi rst minutes after fertilization with the synchronous changes occurring during the fi rst cleavage divisions being the most striking [Buznikov, 1967].…”

Section: The 'Pre-nervous' Serotonergic System In Sea Urchinsmentioning

confidence: 99%

Of Minds and Embryos: Left-Right Asymmetry and the Serotonergic Controls of Pre-Neural Morphogenesis

Levin¹,

2006

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Serotonin is a clinically important neurotransmitter regulating diverse aspects of cognitive function, sleep, mood, and appetite. Increasingly, it is becoming appreciated that serotonin signaling among non-neuronal cells is a novel patterning mechanism existing throughout diverse phyla. Here, we review the evidence implicating serotonergic signaling in embryonic morphogenesis, including gastrulation, craniofacial and bone patterning, and the generation of left-right asymmetry. We propose two models suggesting movement of neurotransmitter molecules as a novel mechanism for how bioelectrical events may couple to downstream signaling cascades and gene activation networks. The discovery of serotonin-dependent patterning events occurring long before the development of the nervous system opens exciting new avenues for future research in evolutionary, developmental, and clinical biology.

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“…Although a significant body of research has focused on the detection of tryptamine and serotonin in sea urchin embryos (Buznikov et al, 2005;Manukhin et al, 1981;Morikawa et al, 2001;Renaud et al, 1983;Shmukler and Tosti, 2001), this marks one of the first studies of 5-HT catabolism in adult sea urchins. Our focus has been on elucidating the 5-HT-related pathways in several deuterostomes.…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, its important positioning in the evolutionary tree, unusual nervous system organization, the essential role it plays in the investigation of fundamental developmental mechanisms and the availability of its genomic information (Burke et al, 2006). The distribution and levels of serotonin and serotonin-like compounds in the embryos of sea urchins have been reported previously (Buznikov et al, 2005;Manukhin et al, 1981;Morikawa et al, 2001;Renaud et al, 1983;Shmukler and Tosti, 2001). Surprisingly, however, little research has focused on the 5-HT pathways in adult sea urchins.…”

Section: Introductionmentioning

confidence: 95%

Serotonin and its metabolism in basal deuterostomes: insights fromStrongylocentrotus purpuratusandXenoturbella bocki

Squires

Rubakhin

Wadhams

et al. 2010

Journal of Experimental Biology

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SUMMARY Serotonin (5-HT), an important molecule in metazoans, is involved in a range of biological processes including neurotransmission and neuromodulation. Both its creation and release are tightly regulated, as is its removal. Multiple neurochemical pathways are responsible for the catabolism of 5-HT and are phyla specific; therefore, by elucidating these catabolic pathways we glean greater understanding of the relationships and origins of various transmitter systems. Here, 5-HT catabolic pathways were studied in Strongylocentrotus purpuratus and Xenoturbella bocki, two organisms occupying distinct positions in deuterostomes. The 5-HTrelated compounds detected in these organisms were compared with those reported in other phyla. In S. purpuratus, 5-HT-related metabolites include N-acetyl serotonin, -glutamyl-serotonin and 5-hydroxyindole acetic acid; the quantity and type were found to vary based on the specific tissues analyzed. In addition to these compounds, varying levels of tryptamine were also seen. Upon addition of a 5-HT precursor and a monoamine oxidase inhibitor, 5-HT itself was detected. In similar experiments using X. bocki tissues, the 5-HT-related compounds found included 5-HT sulfate, -glutamyl-serotonin and 5-hydroxyindole acetic acid, as well as 5-HT and tryptamine. The sea urchin metabolizes 5-HT in a manner similar to both gastropod mollusks, as evidenced by the detection of -glutamyl-serotonin, and vertebrates, as indicated by the presence of 5-hydroxyindole acetic acid and N-acetyl serotonin. In contrast, 5-HT metabolism in X. bocki appears more similar to common protostome 5-HT catabolic pathways.

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On the role of serotonin and 5-methoxy-tryptamine in the regulation of cell division in sea urchin eggs

Cited by 69 publications

References 20 publications

Serotonin stimulates [Ca2+]i elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel

Serotonin stimulates [Ca2+]i elevation in ciliary ectodermal cells of echinoplutei through a serotonin receptor cell network in the blastocoel

Of Minds and Embryos: Left-Right Asymmetry and the Serotonergic Controls of Pre-Neural Morphogenesis

Serotonin and its metabolism in basal deuterostomes: insights fromStrongylocentrotus purpuratusandXenoturbella bocki

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