On the Routines of Wild-Type Silk Fibroin Processing Toward Silk-Inspired Materials: A Review

Volkov, Vadim; Ferreira, Ana Vanessa Fernandes; Cavaco-Paulo, Artur

doi:10.1002/mame.201500179

Cited by 50 publications

(48 citation statements)

References 227 publications

(309 reference statements)

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“…Silk II is an anti-parallel β-sheet structure, belonging to the monoclinic system. Strong hydrogen bonds between adjacent segments contribute greatly to the rigidity and tensile strength of SF [3,4,28]. The silk I structure can be easily converted to silk II via methanol or potassium phosphate treatment [29,30,31].…”

Section: Physicochemical Properties Of Silk Fibroin As Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

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The biological performance of artificial biomaterials is closely related to their structure characteristics. Cell adhesion, migration, proliferation, and differentiation are all strongly affected by the different scale structures of biomaterials. Silk fibroin (SF), extracted mainly from silkworms, has become a popular biomaterial due to its excellent biocompatibility, exceptional mechanical properties, tunable degradation, ease of processing, and sufficient supply. As a material with excellent processability, SF can be processed into various forms with different structures, including particulate, fiber, film, and three-dimensional (3D) porous scaffolds. This review discusses and summarizes the various constructions of SF-based materials, from single structures to multi-level structures, and their applications. In combination with single structures, new techniques for creating special multi-level structures of SF-based materials, such as micropatterning and 3D-printing, are also briefly addressed.

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Section: Physicochemical Properties Of Silk Fibroin As Biomaterialsmentioning

confidence: 99%

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Wang

Wei

et al. 2017

IJMS

404

241

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“…[24] Silk I is transformed into silk II when it passes through the gland of the silkworm, where it is exposed to shear stress and changes in the biochemical environment. [16,24,25] Silk II morphology is found in both native spun fibers as well as reconstituted silk that has undergone treatment to render it insoluble (e.g., methanol treatment, water annealing). [26] A third, distinct polymorph, silk III, can occur at the air/water interface and reflects the amphilicity of silk fibroin.…”

Section: Introductionmentioning

confidence: 99%

Experimental Methods for Characterizing the Secondary Structure and Thermal Properties of Silk Proteins

McGill

Holland

Kaplan

2018

Macromol. Rapid Commun.

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Silk proteins are biopolymers produced by spinning organisms that have been studied extensively for applications in materials engineering, regenerative medicine, and devices due to their high tensile strength and extensibility. This remarkable combination of mechanical properties arises from their unique semi-crystalline secondary structure and block copolymer features. The secondary structure of silks is highly sensitive to processing, and can be manipulated to achieve a wide array of material profiles. Studying the secondary structure of silks is therefore critical to understanding the relationship between structure and function, the strength and stability of silk-based materials, and the natural fiber synthesis process employed by spinning organisms. However, silks present unique challenges to structural characterization due to high-molecular-weight protein chains, repetitive sequences, and heterogeneity in intra- and interchain domain sizes. Here, experimental techniques used to study the secondary structure of silks, the information attainable from these techniques, and the limitations associated with them are reviewed. Ultimately, the appropriate utilization of a suite of techniques discussed here will enable detailed characterization of silk-based materials, from studying fundamental processing-structure-function relationships to developing commercially useful quality control assessments.

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“…Silk I contains the random coil and amorphous structures, and silk II has an anti-parallel β-sheet structure with hydrogen bonds [35]. These strong hydrogen bonds with adjacent chains contributes to the mechanical and tensile strength of SF [16,35,37].…”

Section: Fibroinmentioning

confidence: 99%

Silk Protein-Based Membrane for Guided Bone Regeneration

Kwon

Seok

2018

Applied Sciences

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Silk derived from the silkworm is known for its excellent biological and mechanical properties. It has been used in various fields as a biomaterial, especially in bone tissue engineering scaffolding. Recently, silk protein-based biomaterial has been used as a barrier membrane scaffolding for guided bone regeneration (GBR). GBR promotes bone regeneration in bone defect areas using special barrier membranes. GBR membranes should have biocompatibility, biodegradability, cell occlusion, the mechanical properties of space-making, and easy clinical handling. Silk-based biomaterial has excellent biologic and mechanical properties that make it a good candidate to be used as GBR membranes. Recently, various forms of silk protein-based membranes have been introduced, demonstrating excellent bone regeneration ability, including osteogenic cell proliferation and osteogenic gene expression, and promoting new bone regeneration in vivo. In this article, we introduced the characteristics of silk protein as bone tissue engineering scaffolding and the recent application of such silk material as a GBR membrane. We also suggested future studies exploring additional uses of silk-based materials as GBR membranes.

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On the Routines of Wild-Type Silk Fibroin Processing Toward Silk-Inspired Materials: A Review

Cited by 50 publications

References 227 publications

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

A Review of Structure Construction of Silk Fibroin Biomaterials from Single Structures to Multi-Level Structures

Experimental Methods for Characterizing the Secondary Structure and Thermal Properties of Silk Proteins

Silk Protein-Based Membrane for Guided Bone Regeneration

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