On the size-dependent internalization of sub-hundred polymeric nanoparticles

Castro, Joao Vieira De; Reis, Rui L.; Ferreira, Helena; Neves, Nuno M.

doi:10.1016/j.colsurfb.2023.113245

Cited by 13 publications

(8 citation statements)

References 46 publications

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“…These results are corroborated by our recent study about the cellular uptake of NPs. Results reported the same size dependency in internalization rate for the same cell line (EA.hy926) and NP formulations . Indeed, we observed that NP-FITC undergo quick internalization in endothelial cells (30 > 50 > 70 nm).…”

Section: Resultssupporting

confidence: 78%

“…Results reported the same size dependency in internalization rate for the same cell line (EA.hy926) and NP formulations. 37 Indeed, we observed that NP-FITC undergo quick internalization in endothelial cells (30 > 50 > 70 nm). Hence, similar events should be observed in NPs crossing through the endothelial barrier.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

“…The most interesting range of NP sizes for intracellular delivery is below 100 nm . Moreover, research studies reported that even minor variations in size in the subhundred nanometer range can have a substantial impact upon the way NPs interact with cells and other biological structures. − However, many conventional strategies for synthesizing NPs lack accurate control over the experimental parameters. This generally leads to the production of NPs with inconsistent or unpredictable sizes.…”

Section: Introductionmentioning

confidence: 99%

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Size-Dependent Polymeric Nanoparticle Distribution in a Static versus Dynamic Microfluidic Blood Vessel Model: Implications for Nanoparticle-Based Drug Delivery

Ferreira

Reis

Neves

2023

ACS Appl. Nano Mater.

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Nanoparticles (NPs) have been widely investigated in the nanomedicine field. One of the main challenges is to accurately predict the NP distribution and fate after administration. Microfluidic platforms acquired huge importance as tools to model the in vivo environment. In this study, we leveraged a microfluidic platform to produce FITC-labeled poly(lactide-coglycolide)-block-poly(ethylene glycol) (PLGA-PEG) NPs with defined sizes of 30, 50, and 70 nm. The study aimed to compare the ability of NPs with differences of 20 nm in size to cross an endothelial barrier using static (Transwell inserts) and dynamic (microfluidic perfusion device) in vitro models. Our results evidence a size-dependent NP crossing in both models (30 > 50 > 70 nm) and highlight the bias deriving from the static model, which does not involve shear stresses. The permeation of each NP size was significantly higher in the static system than in the dynamic model at the earliest stages. However, it gradually decreased to levels comparable with those of the dynamic model. Overall, this work highlights clear differences in NP distribution over time in static versus dynamic conditions and distinct size-dependent patterns. These findings reinforce the need for accurate in vitro screening models that allow for more accurate predictions of in vivo performance.

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Section: Resultssupporting

confidence: 78%

Section: ■ Results and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Size-Dependent Polymeric Nanoparticle Distribution in a Static versus Dynamic Microfluidic Blood Vessel Model: Implications for Nanoparticle-Based Drug Delivery

Ferreira

Reis

Neves

2023

ACS Appl. Nano Mater.

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“…For instance, the ability to finely tune experimental parameters allows for the production of highly monodisperse NPs, ensuring consistent properties within or between different batches. The importance of uniformity becomes particularly significant in applications where particle characteristics directly influence performance or desired outcomes as in nanomedicine . Furthermore, microfluidics advances have facilitated in situ NPs characterization by seamlessly integrating advanced analysis techniques, such as synchrotron small-angle X-ray scattering (SAXS) .…”

Section: Microfluidic Devicesmentioning

confidence: 99%

“…The importance of uniformity becomes particularly significant in applications where particle characteristics directly influence performance or desired outcomes as in nanomedicine. 69 Furthermore, microfluidics advances have facilitated in situ NPs characterization by seamlessly integrating advanced analysis techniques, such as synchrotron small-angle X-ray scattering (SAXS). 70 This emerging application involves the combination of specially designed microfluidic devices with SAXS, creating a platform that allows for real-time detection of dynamic structural changes during the production of NPs.…”

Section: Microfluidic Devicesmentioning

confidence: 99%

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

Gimondi,

Ferreira,

Reis

et al. 2023

ACS Nano

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The use of nanoparticles (NPs) in nanomedicine holds great promise for the treatment of diseases for which conventional therapies present serious limitations. Additionally, NPs can drastically improve early diagnosis and follow-up of many disorders. However, to harness their full capabilities, they must be precisely designed, produced, and tested in relevant models. Microfluidic systems can simulate dynamic fluid flows, gradients, specific microenvironments, and multiorgan complexes, providing an efficient and cost-effective approach for both NPs synthesis and screening. Microfluidic technologies allow for the synthesis of NPs under controlled conditions, enhancing batch-to-batch reproducibility. Moreover, due to the versatility of microfluidic devices, it is possible to generate and customize endless platforms for rapid and efficient in vitro and in vivo screening of NPs’ performance. Indeed, microfluidic devices show great potential as advanced systems for small organism manipulation and immobilization. In this review, first we summarize the major microfluidic platforms that allow for controlled NPs synthesis. Next, we will discuss the most innovative microfluidic platforms that enable mimicking in vitro environments as well as give insights into organism-on-a-chip and their promising application for NPs screening. We conclude this review with a critical assessment of the current challenges and possible future directions of microfluidic systems in NPs synthesis and screening to impact the field of nanomedicine.

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Unlocking the Mitochondria for Nanomedicine-based Treatments: Overcoming Biological Barriers, Improving Designs, and Selecting Verification Techniques

Pegoraro,

Domingo-Ortí,

Conejos-Sánchez

et al. 2024

Advanced Drug Delivery Reviews

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On the size-dependent internalization of sub-hundred polymeric nanoparticles

Cited by 13 publications

References 46 publications

Size-Dependent Polymeric Nanoparticle Distribution in a Static versus Dynamic Microfluidic Blood Vessel Model: Implications for Nanoparticle-Based Drug Delivery

Size-Dependent Polymeric Nanoparticle Distribution in a Static versus Dynamic Microfluidic Blood Vessel Model: Implications for Nanoparticle-Based Drug Delivery

Microfluidic Devices: A Tool for Nanoparticle Synthesis and Performance Evaluation

Unlocking the Mitochondria for Nanomedicine-based Treatments: Overcoming Biological Barriers, Improving Designs, and Selecting Verification Techniques

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